Production method of probiotic active milk beverage rich in organic selenium
A production method and technology for probiotics, applied in dairy products, applications, milk preparations, etc., can solve the problems of limited transformation capacity, influence on the growth of probiotics, and high proportions, and achieve beneficial proliferation, good fermentation, and strong selenium-enriched ability. Effect
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Embodiment 1
[0051] Fermentation of selenium-enriched yeast with high biomass and high selenium content:
[0052] (1) Fermentation and cultivation of selenium-enriched yeast:
[0053] (1), culture of slant strains: selenium-enriched yeast strains were inoculated on solid wort slant surfaces containing a total sugar content of 8% and a sodium selenite concentration of 50 micrograms / ml, and cultivated at 32°C for 24 hours. Store at 4°C.
[0054] (2) Liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort medium containing 8% total sugar content and 10 μg / ml sodium selenite concentration In a Erlenmeyer flask, shake culture at 32°C for 16 hours, which is the liquid strain.
[0055] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort medium with a total sugar content of 8% and a sodium selenite concentration of 50 micrograms / ml by a volume inoculum of 5%. , shake culture at 3...
Embodiment 2
[0070] Fermentation of selenium-enriched yeast with high biomass and high selenium content:
[0071] (1) Fermentation and cultivation of selenium-enriched yeast:
[0072] (1), slant strain culture: selenium-enriched yeast strains were inoculated on the wort solid slant surface containing 20% total sugar content and 100 μg / ml sodium selenite concentration, and cultured at 25°C for 24 hours. Store at 4°C.
[0073] (2) Liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort culture medium with a total sugar content of 20% and a sodium selenite concentration of 20 micrograms per milliliter In a Erlenmeyer flask, shake culture at 25°C for 24 hours, which is the liquid strain.
[0074] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort culture medium that the total sugar content is 20% and the sodium selenite concentration is 100 micrograms per milliliter accordi...
Embodiment 3
[0090] (1) Fermentation and cultivation of selenium-enriched yeast:
[0091] (1) Slant strain culture: Selenium-enriched yeast strains were inoculated on solid wort slant surfaces containing a total sugar content of 10% and a sodium selenite concentration of 80 micrograms / ml, and cultivated at 30°C for 36 hours. Store at 4°C.
[0092] (2), liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort medium containing 10% total sugar content and 20 μg / ml sodium selenite concentration In a Erlenmeyer flask, shake culture at 30°C for 20 hours, which is the liquid strain.
[0093] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort culture medium that the total sugar content is 10% and the sodium selenite concentration is 80 micrograms / ml according to the volume inoculation amount of 10%. , shake culture at 30°C for 14 hours, which is the primary liquid seed culture. ...
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