Production method of probiotic active milk beverage rich in organic selenium

A production method and technology for probiotics, applied in dairy products, applications, milk preparations, etc., can solve the problems of limited transformation capacity, influence on the growth of probiotics, and high proportions, and achieve beneficial proliferation, good fermentation, and strong selenium-enriched ability. Effect

Inactive Publication Date: 2013-10-02
山东硒莱姆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] At present, there have been literature records of converting inorganic selenium into organic selenium, such as the patent document No. 2006100972884, which records a production method of probiotic yoghurt rich in selenium amino acids, which adopts the addition of selenous acid in the probiotic culture medium Sodium solution (inorganic selenium), which converts inorganic selenium into selenoamino acids through probiotic fermentation, has the problem that due to probiotics such as Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus acidophilus, and Streptococcus thermophilus The ability to convert inorganic selenium is limited, so the conversion rate of inorganic selenium achieved in this patent is only 50%. Since there is still unconverted inorganic selenium in the finished product, and the proportion is very high, it has certain toxicity
And because the patent uses sodium selenite inorganic selenium as the raw material for probiotic fermentation, sodium selenite has a certain influence on the growth of probiotics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Fermentation of selenium-enriched yeast with high biomass and high selenium content:

[0052] (1) Fermentation and cultivation of selenium-enriched yeast:

[0053] (1), culture of slant strains: selenium-enriched yeast strains were inoculated on solid wort slant surfaces containing a total sugar content of 8% and a sodium selenite concentration of 50 micrograms / ml, and cultivated at 32°C for 24 hours. Store at 4°C.

[0054] (2) Liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort medium containing 8% total sugar content and 10 μg / ml sodium selenite concentration In a Erlenmeyer flask, shake culture at 32°C for 16 hours, which is the liquid strain.

[0055] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort medium with a total sugar content of 8% and a sodium selenite concentration of 50 micrograms / ml by a volume inoculum of 5%. , shake culture at 3...

Embodiment 2

[0070] Fermentation of selenium-enriched yeast with high biomass and high selenium content:

[0071] (1) Fermentation and cultivation of selenium-enriched yeast:

[0072] (1), slant strain culture: selenium-enriched yeast strains were inoculated on the wort solid slant surface containing 20% ​​total sugar content and 100 μg / ml sodium selenite concentration, and cultured at 25°C for 24 hours. Store at 4°C.

[0073] (2) Liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort culture medium with a total sugar content of 20% and a sodium selenite concentration of 20 micrograms per milliliter In a Erlenmeyer flask, shake culture at 25°C for 24 hours, which is the liquid strain.

[0074] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort culture medium that the total sugar content is 20% and the sodium selenite concentration is 100 micrograms per milliliter accordi...

Embodiment 3

[0090] (1) Fermentation and cultivation of selenium-enriched yeast:

[0091] (1) Slant strain culture: Selenium-enriched yeast strains were inoculated on solid wort slant surfaces containing a total sugar content of 10% and a sodium selenite concentration of 80 micrograms / ml, and cultivated at 30°C for 36 hours. Store at 4°C.

[0092] (2), liquid strain culture: After activating the preserved selenium-enriched yeast strains, connect a ring of cells to wort medium containing 10% total sugar content and 20 μg / ml sodium selenite concentration In a Erlenmeyer flask, shake culture at 30°C for 20 hours, which is the liquid strain.

[0093] (3), first-level liquid seed culture: the liquid strain is inserted into the Erlenmeyer flask of the wort culture medium that the total sugar content is 10% and the sodium selenite concentration is 80 micrograms / ml according to the volume inoculation amount of 10%. , shake culture at 30°C for 14 hours, which is the primary liquid seed culture. ...

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PUM

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Abstract

The invention belongs to the technical field of active milk beverage preparation, and particularly relates to a production method of a probiotic active milk beverage rich in organic selenium. Sodium selenite is added in different stages, so that the best combining condition between yeast and selenium is optimized; the selenium combining quantity is increased; the growth of the growth is facilitated; inorganic selenium is combined into organic selenium; the yeast is separated by a sheet frame press filtration or centrifugal method; selenium-enriched yeast powder is obtained by drying and deactivation, and serves as a raw material for secondary fermentation via probiotics such as bifidobacterium bifidum, lactobacillus bulgaricus, lactobacillus acidophilus, thermophilic streptobacillus and bacillus cereus, and secondary conversion via microbial enzyme; and inorganic selenium left in a yeast cell is converted into selenium protein and seleniferous protein basically completely. Therefore, the probiotic functional food beverage rich in organic selenium is obtained. The product can exert comprehensive health-care effects of the probiotics, the yeast and organic selenium.

Description

technical field [0001] The invention belongs to the technical field of active milk beverage preparation, in particular to a production method of probiotic active milk beverage rich in organic selenium. Background technique [0002] Selenium is an essential trace element for organisms, which cannot be synthesized in the human body. The total selenium content of the human body is 6-20mg. Selenium is distributed throughout various tissues, organs and body fluids, with the highest concentration in the kidney. The selenium in the male body is mostly concentrated in the testis and the vas deferens of the prostate, and will be excreted together with the semen. Since there are no long-term selenium storage organs in the human body, the selenium needed by the body should be continuously supplemented by sufficient selenium from the diet. The balance of selenium concentration has an important protective and promoting effect on the physiological functions of many organs and tissues. W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/127
Inventor 谷劲松
Owner 山东硒莱姆生物科技有限公司
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