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A kind of cultivation method of selenium-enriched microalgae

A cultivation method and technology of microalgae, applied in the field of cultivation of selenium-enriched microalgae, can solve problems such as poor selenium-enrichment ability, and achieve the effect of rich nutritional value, easy method and low cost

Active Publication Date: 2021-04-20
恩施硒圣植物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, most of the existing microalgae do not have strong selenium-enriching ability. How to develop a culture method for artificial breeding to make microalgae grow rapidly and have strong selenium-enriching ability has become a technical problem to be solved urgently.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for cultivating selenium-enriched microalgae, said method comprising the steps of:

[0022] (1) Prepare culture medium: the composition of culture medium is sodium bicarbonate 28mg / L, disodium hydrogen phosphate 70mg / L, ferric ammonium citrate 5mg / L, calcium chloride 210mg / L, magnesium sulfate 75mg / L, boric acid 1.8mg / L, manganese chloride 1.8mg / L, zinc sulfate 0.4mg / L, copper sulfate 0.08mg / L.

[0023] (2) Stable culture medium: add hydrochloric acid to the prepared culture medium to adjust the pH value to 5.7, pressurize to 10Mpa by air, and maintain the pressure for 30min.

[0024] (3) Inoculation and cultivation: take microalgae strains (purchased Chlorella), inoculate at a ratio of 1:900 (w / w) (that is, inoculate 1 g of microalgae strains per 900 g of culture solution); 25°C) conditions. During the cultivation process, the light was given according to the natural daylight, and the cultivation was carried out for 10 days.

[0025] (4) Selenium-enriched ...

Embodiment 2

[0030] A method for cultivating selenium-enriched microalgae, said method comprising the steps of:

[0031] (1) Prepare culture medium: the composition of culture medium is sodium bicarbonate 21mg / L, disodium hydrogen phosphate 67mg / L, ferric ammonium citrate 3mg / L, calcium chloride 180mg / L, magnesium sulfate 60mg / L, boric acid 1.5mg / L, manganese chloride 1.3mg / L, zinc sulfate 0.2mg / L, copper sulfate 0.05mg / L.

[0032] (2) Stable culture medium: Add hydrochloric acid to the prepared culture medium to adjust the pH value to 5.5, pressurize to 9.5Mpa by air, and maintain the pressure for 40 minutes.

[0033] (3) Inoculation culture: take microalgae strains (purchased Chlorella), inoculate at a ratio of 1:1000 (w / w) (that is, inoculate 1 g of microalgae strains per 1000 g of culture solution); 25°C) conditions. During the cultivation process, the light was given according to the natural daylight, and the cultivation was carried out for 7 days.

[0034] (4) Selenium-enriched c...

Embodiment 3

[0039] A method for cultivating selenium-enriched microalgae, said method comprising the steps of:

[0040] (1) Prepare culture medium: The culture medium is composed of sodium bicarbonate 34mg / L, disodium hydrogen phosphate 74mg / L, ferric ammonium citrate 7mg / L, calcium chloride 240mg / L, magnesium sulfate 90mg / L, boric acid 2mg / L L, manganese chloride 3.0mg / L, zinc sulfate 0.5mg / L, copper sulfate 0.1mg / L.

[0041] (2) Stable culture medium: Add hydrochloric acid to the prepared culture medium to adjust the pH value to 5.9, pressurize to 10.5Mpa by air, and maintain the pressure for 20 minutes.

[0042] (3) Inoculation and cultivation: take microalgae strains (purchased Chlorella), inoculate at a ratio of 1:800 (w / w) (that is, inoculate 1 g of microalgae strains per 800 g of culture solution); 25°C) conditions. During the cultivation process, the light was given according to the natural daylight, and the cultivation was carried out for 15 days.

[0043] (4) Selenium-enriche...

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Abstract

The invention provides a method for cultivating selenium-enriched microalgae. (1) Preparation of culture solution: sodium bicarbonate 21-34 mg / L, disodium hydrogen phosphate 67-74 mg / L, ferric ammonium citrate 3-7 mg / L, Calcium chloride 180~240mg / L, magnesium sulfate 60~90mg / L, boric acid 1.5~2mg / L, manganese chloride 1.3~3.0mg / L, zinc sulfate 0.2~0.5mg / L, copper sulfate 0.05~0.1mg / L L; (2) adjusting the pH to 5.5-5.9; (3) inserting microalgae strains and culturing; (4) adding sodium selenite and culturing under air pressure; (5) harvesting. The method is easy to implement, easy to operate, rich in nutrition, low in cost, rapid in growth of microalgae, strong in selenium enrichment ability, selenium content in 1200ppm-2400ppm, wherein protein content reaches more than 46%, and rich in nutritional value.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a method for cultivating selenium-enriched microalgae. Background technique [0002] Microalgae is not a taxonomic term, but refers to tiny groups of algae whose shape can only be discerned under a microscope. Microalgae generally refers to the general term for microorganisms that contain chlorophyll a and can perform photosynthesis, and belong to a kind of protozoa. Widely distributed on land and in the sea, autotrophic plants with rich nutrition and high photosynthetic utilization, polysaccharides, proteins, pigments, etc. produced by cell metabolism, make them have good development prospects in the fields of food, medicine, genetic engineering, liquid fuels, etc. . At present, microalgae that are mass-cultivated or produced by biotechnology belong to four phyla: Cyanophyta, Chlorophyta, Chrysophyta and Rhodophyta. [0003] There are many kinds of microalgae, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38
Inventor 张新江
Owner 恩施硒圣植物科技有限公司
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