Method for improving quality of the tobacco leaf expanded cut stem by microorganism enzyme

Abstract

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r / min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U / L of Lip, 11U / L of MnP and 5.6U / L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

Description

technical field [0001] The invention relates to the technical field of cut tobacco stem treatment, in particular to a method for improving the quality of expanded cut stems of tobacco leaves by using microbial enzymes. Background technique [0002] With the development of cigarette production technology, adding shredded stems to the main raw material shredded tobacco can significantly improve the single weight, draw resistance, mainstream smoke and sensory quality of cigarettes, and the specific performance is that the single cigarette weight and draw resistance are on a downward trend; The purpose of reducing the total particulate matter, tar and CO in mainstream smoke of cigarettes can be achieved. Therefore, adding a certain amount of shredded stems to cigarette raw materials has become one of the key technical links generally adopted by domestic and foreign cigarette manufacturers in order to further improve the quality of cigarettes and reduce tar and harm. However, du...

AI Technical Summary

Problems solved by technology

However, due to the physical properties and main chemical components (mainly cellulose, hemicellulose, pectin, lignin, etc.) Defects of decreased and increased woody taste, thus hindering the effective utilization of cut stems in cigarette raw materials

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