Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation
A microbial inoculum, solid-state fermentation technology, applied in the fields of saccharification enzyme, phytase, lipase, vegetase, production of multi-enzyme high-protein feed, beta-amylase, pectinase, and can solve the utilization rate of active ingredients It can improve the intestinal microecology, reduce the ratio of feed to meat, and promote rapid growth.
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Embodiment 2
[0010] Embodiment two. First, prepare the seed medium according to the following four preparation methods: 1. Take 20g of cornmeal, add water to 100ml, adjust the pH to 6.8 with NaOH, heat to 90°C, add α-amylase at a ratio of 10u / gram of cornmeal, and continue to keep warm After 25-35 minutes, cool down to 30°C to prepare the first seed medium, hereinafter referred to as M1. 2. Take 10g of soybean meal, add 2% of glucose and 0.1% of KH to the weight of soybean meal 2 PO 4 , 0.05% MgSO 4 , add water to 100ml, adjust the pH to 7.0-7.2, sterilize at 121°C for 10-15 minutes, cool to 30°C, and prepare the second seed medium, hereinafter referred to as M2. 3. By glucose 5.0% , (NH 4 ) 2 SO 4 0.4%, corn steep liquor 1.2%, KH 2 PO 4 0.05%, K 2 HPO 4 0.05%, MgSO 4 0.04%, V B 50ug / 100ml, V H Mix the ratio of 50ug / 100ml and the rest of water to 100ml, adjust the pH to 7.0-7.2, sterilize at 121°C for 10-15 minutes, and cool to 30°C to prepare the third seed medium, hereinafter...
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