Method for producing multi-vitamins and multi-enzymes high protein microorganic agent by multi-bacteria solid fermentation

A microbial inoculum, solid-state fermentation technology, applied in the fields of saccharification enzyme, phytase, lipase, vegetase, production of multi-enzyme high-protein feed, beta-amylase, pectinase, and can solve the utilization rate of active ingredients It can improve the intestinal microecology, reduce the ratio of feed to meat, and promote rapid growth.

Inactive Publication Date: 2004-12-08
哈尔滨中冠生物工程有限公司
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Problems solved by technology

In this way, not only the utilization rate of active ingredients in the feed is low, but also a lot of waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0010] Embodiment two. First, prepare the seed medium according to the following four preparation methods: 1. Take 20g of cornmeal, add water to 100ml, adjust the pH to 6.8 with NaOH, heat to 90°C, add α-amylase at a ratio of 10u / gram of cornmeal, and continue to keep warm After 25-35 minutes, cool down to 30°C to prepare the first seed medium, hereinafter referred to as M1. 2. Take 10g of soybean meal, add 2% of glucose and 0.1% of KH to the weight of soybean meal 2 PO 4 , 0.05% MgSO 4 , add water to 100ml, adjust the pH to 7.0-7.2, sterilize at 121°C for 10-15 minutes, cool to 30°C, and prepare the second seed medium, hereinafter referred to as M2. 3. By glucose 5.0% , (NH 4 ) 2 SO 4 0.4%, corn steep liquor 1.2%, KH 2 PO 4 0.05%, K 2 HPO 4 0.05%, MgSO 4 0.04%, V B 50ug / 100ml, V H Mix the ratio of 50ug / 100ml and the rest of water to 100ml, adjust the pH to 7.0-7.2, sterilize at 121°C for 10-15 minutes, and cool to 30°C to prepare the third seed medium, hereinafter...

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Abstract

A process for preparing the multi-vitamine multi-enzyme high-protein microbial preparation by the solid fermentation of multiple bacterial strains includes such steps as preparing seed culture media, respectively inoculating reticularia, torula yeast, glutamic corynebacteria, Bacillus subtitis, Aspergillus niger, etc into said seed culture media, culturing at 30-35 deg.C for 15-18 hr, inoculating them into the solid culture medium, fermenting at 33+/-0.5 deg.C for 70-80 hr, and fast drying. Its can be used as the feed containing living microbes, microbial enzymes, vitamines, etc.

Description

[0001] (1) Fields [0002] The invention relates to a microbial solid-state fermentation technology, in particular to a method for producing multi-dimensional, multi-enzyme and high-protein feed by adopting multi-strain mixed culture and solid-state fermentation. (two), background technology [0003] Corn, bran, distiller's grains, cakes, crop residues, and grains are commonly used feedstuffs. These feeds are often directly fed to various animals after simple crushing, cooking and other processing. In this way, not only the utilization rate of active ingredients in the feed is low, but also a large amount of waste is caused. Moreover, long-term consumption of such feed by animals will cause malnutrition and low immunity. Slow down the growth rate of the animal or reduce the egg production rate. (3) Contents of the invention [0004] The purpose of the present invention is to provide a multi-species solid-state fermentation production of multi-dimensional multi-enzyme high...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/30A23K10/37A23K40/00C12N1/14C12P1/02
CPCY02P60/87
Inventor 梁金钟张智赵云财吕伟民麻名汉范洪臣陈成徐速
Owner 哈尔滨中冠生物工程有限公司
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