Compound preparation for improving digestibility of dry coconut shreds and feed additive

Abstract

The invention belongs to the technical field of feed additives, and particularly provides a compound preparation for improving the digestibility of dry coconut shreds, which comprises a compound enzyme preparation, the compound enzyme preparation comprises xylanase with the enzyme activity of 0.5 million U / g to 25 million U / g, glucanase with the enzyme activity of 0.8 million U / g to 200 million U / g, mannase with the enzyme activity of 10,000 U / g to 200 million U / g, cellulase with the enzyme activity of 1.2 million U / g to 25 million U / g, pectinase with the enzyme activity of 10,000 U / g to 25 million U / g, galactosidase with the enzyme activity of 10,000 U / g to 300 million U / g and phytase with the enzyme activity of 0.8 million U / g to 200 million U / g. According to the compound preparation provided by the invention, macromolecular substances such as starch in the dry coconut shreds can be degraded into micromolecular substances which are easy to digest, so that the digestion utilization rate is greatly increased; fat is degraded, fatty acid components such as palmitic acid, oleic acid and lauric acid in the coconut shreds are released, and the effects of reducing blood pressure, promoting digestion and the like are achieved; meanwhile, the generated active substances can improve the disease resistance of animals, improve the health state of organisms, improve the nutritional value of the dried shredded coconut and increase the variety of unconventional feed raw materials.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a composite preparation and a feed additive for improving the digestibility of dried coconut shreds. Background Art

[0002] Feed raw materials can be divided into many types according to their composition and use, mainly including roughage (natural moisture content exceeds 60%), green feed (moisture content is generally between 60% and 80%, and crude fiber content is generally between 15% and 30%), silage (the ingredients mainly include water, dry matter, crude protein, crude fat, crude fiber and nitrogen-free extract, etc.), energy feed (crude fiber content in dry matter is less than 18%, and crude protein content is less than 20%), protein supplement (crude fiber content in dry matter is less than 18%, but crude protein content is greater than or equal to 20%), mineral feed (providing minerals such as calcium, phosphorus, salt, copper, iron, zinc, manganese, etc.), vitamin feed (composed of industrially synthesized single or compound vitamins) and feed additives (trace substances used to improve feed quality and promote animal growth and health).

[0003] Dried coconut shreds are different from traditional feed raw materials and are potential feed resources. Dried coconut shreds are made from coconut meat of coconut fruit after slicing, grinding or shaving into thin slices and then dehydrating and drying. They have the unique aroma of coconut. The main components of dried coconut shreds include moisture (3%-5%), protein (about 5%), fat (more than 45%), crude fiber (more than 40%), dietary fiber (more than 30%), ash (0.5%-1.5%), vitamins and minerals. It can be seen that dried coconut shreds have low moisture, protein and ash content, high fat and cellulose content, but its dietary fiber content is higher than traditional feed raw materials. Dietary fiber has many benefits for health, including promoting digestion, controlling blood sugar, lowering cholesterol, etc., promoting animal intestinal movement, increasing feces volume, slowing down feces flow, and helping excretion and detoxification. Coconut shreds have a high oil content and are rich in medium-chain fatty acids, which can be directly absorbed and utilized by the animal intestines and converted into energy. Therefore, coconut shreds can become a very good source of energy and provide high-energy feed for animals. Coconut shreds are a renewable resource. Their production and utilization have relatively little impact on the environment and can effectively reduce environmental pollution. They are also a low-cost feed raw material and their utilization can bring considerable economic benefits to farmers.

[0004] At present, the main application of dried coconut shreds is to dry them and make them into food supplements. There is biological fermentation of dried coconut shreds applied to the feed industry, but they are not directly used as animal feed. As a very high-quality feed raw material, the application of dried coconut shreds in livestock and poultry feed has attracted more and more attention. Dried coconut shreds can provide high energy, high protein and crude fiber, which is very beneficial to the growth and development of livestock and poultry. At the same time, coconut shreds can also improve the environment and increase economic benefits. It is a feed raw material that is very worthy of promotion. Summary of the invention

[0005] The invention aims to degrade the fat in the dried coconut shreds to provide rich medium-chain fatty acids and short-chain fatty acids, which can be directly absorbed and utilized by the intestinal tract of animals, eliminate the anti-nutritional properties of non-starch polysaccharides such as fiber, improve the nutritional value of the dried coconut shreds, provide a low-cost, renewable, high-quality feed raw material, increase the types of unconventional feed raw materials, and obtain better economic benefits.

[0006] To this end, the invention provides a composite preparation for improving the digestibility of dried coconut shreds, comprising a composite enzyme preparation; the composite enzyme preparation comprises xylanase with an enzyme activity of 5,000-250,000 U / g, glucanase with an enzyme activity of 8,000-200,000 U / g, mannanase with an enzyme activity of 10,000-200,000 U / g, cellulase with an enzyme activity of 12,000-250,000 U / g, pectinase with an enzyme activity of 10,000-250,000 U / g, galactosidase with an enzyme activity of 10,000-300,000 U / g, and phytase with an enzyme activity of 8,000-200,000 U / g.

[0007] Specifically, the above-mentioned composite preparation also includes lipase with an enzyme activity of 100,000-300,000 U / g.

[0008] Specifically, the above-mentioned composite preparation includes xylanase with an enzyme activity of 8,000 U / g, glucanase with an enzyme activity of 9,000 U / g, mannanase with an enzyme activity of 15,000 U / g, cellulase with an enzyme activity of 200,000 U / g, pectinase with an enzyme activity of 30,000 U / g, galactosidase with an enzyme activity of 10,000 U / g, phytase with an enzyme activity of 8,000 U / g, and lipase with an enzyme activity of 250,000 U / g.

[0009] Specifically, the above-mentioned composite preparation also includes an emulsifier; the added amount of the emulsifier is 10%.

[0010] Specifically, the emulsifier includes at least one of sucrose fatty acid ester and quartz powder.

[0011] Specifically, in terms of mass percentage, the emulsifier includes 65% sucrose fatty acid ester and 35% quartz powder.

[0012] The present invention also provides a feed additive, comprising: dried coconut shreds and any one of the above composite preparations.

[0013] Specifically, the addition amount of the above-mentioned composite preparation is 100-300g / t of the mass of dry coconut shreds.

[0014] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0015] The composite preparation for improving the digestibility of dried coconut shreds provided by the invention can degrade macromolecular substances such as starch in dried coconut shreds into easily digestible small molecular substances, thereby greatly improving the digestion utilization rate; degrading fat to release fatty acid components such as palmitic acid, oleic acid, lauric acid, etc. in the coconut shreds has the effects of lowering blood pressure and promoting digestion; and the active substances generated at the same time can improve the disease resistance of animals, improve the health status of the body, improve the nutritional value of dried coconut shreds, and increase the types of unconventional feed raw materials. DETAILED DESCRIPTION

[0016] The technical scheme in the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Although the representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes can be made to the present invention without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be limited by the attached claims and their equivalents.

[0017] The invention provides a composite preparation for improving the digestibility of dried coconut shreds, comprising a composite enzyme preparation; the composite enzyme preparation comprises xylanase with an enzyme activity of 5,000-250,000 U / g, glucanase with an enzyme activity of 8,000-200,000 U / g, mannanase with an enzyme activity of 10,000-200,000 U / g, cellulase with an enzyme activity of 12,000-250,000 U / g, pectinase with an enzyme activity of 10,000-250,000 U / g, galactosidase with an enzyme activity of 10,000-300,000 U / g, and phytase with an enzyme activity of 8,000-200,000 U / g.

[0018] The composite preparation mainly contains cellulase and pectinase, which can destroy the cell wall of shredded coconut, release the nutrients in the cells, and make them easy to be contacted by digestive enzymes. It can also eliminate the anti-nutritional factors in shredded coconut, reduce the viscosity of gastrointestinal contents, and promote digestion and absorption. Through the synergistic action of glucanase, xylanase, mannanase, and galactosidase, the anti-nutritional effect of non-starch polysaccharides is eliminated; phytase can convert phytic acid-bound phosphorus into effective phosphorus for animal use, thereby improving the utilization rate of phosphorus in feed and reducing the pollution of fecal phosphorus to the water environment.

[0019] Furthermore, the composite preparation also includes lipase with an enzyme activity of 100,000-300,000 U / g.

[0020] Furthermore, the composite preparation also includes an emulsifier; the added amount of the emulsifier is 10%.

[0021] Through the synergistic effect of lipase and emulsifier, the fat in dry coconut shreds can be directly digested and hydrolyzed, reducing the hindering effect of fat on the digestion and absorption of other nutrients, improving the digestion and utilization rate of other nutrients, and at the same time releasing fatty acid components such as palmitic acid, oleic acid, and lauric acid in coconut shreds. When used as a feed additive, it has the effects of lowering blood pressure and promoting digestion.

[0022] The present invention also provides a feed additive, comprising: dried coconut shreds and any one of the above composite preparations.

[0023] Specifically, the addition amount of the above-mentioned composite preparation is 100-300g / t of the mass of dry coconut shreds.

[0024] The effects of the composite preparation and feed additive for improving the digestibility of shredded coconut of the present invention are studied by means of specific examples.

[0025] The ingredients of the dried coconut shreds used in the embodiment of the present invention are shown in Table 1. The moisture and ash contents are relatively low, and the fat and fiber contents are relatively high, which are 49.12% and 42.21, respectively. However, the total dietary fiber content is 33.51%, which is significantly different from the currently used feed raw materials. It can provide the crude fiber required by livestock and poultry to help digestion and excretion.

[0026] Table 1 Ingredients of shredded coconut

[0027]

[0028] Embodiment 1:

[0029] The present embodiment provides a composite preparation for improving the digestibility of dried coconut shreds, including xylanase with an enzyme activity of 8,000 U / g, glucanase with an enzyme activity of 9,000 U / g, mannanase with an enzyme activity of 15,000 U / g, cellulase with an enzyme activity of 200,000 U / g, pectinase with an enzyme activity of 30,000 U / g, galactosidase with an enzyme activity of 10,000 U / g, and phytase with an enzyme activity of 8,000 U / g.

[0030] Embodiment 2:

[0031] The present embodiment provides a composite preparation for improving the digestibility of dried coconut shreds, including xylanase with an enzyme activity of 8,000 U / g, glucanase with an enzyme activity of 9,000 U / g, mannanase with an enzyme activity of 15,000 U / g, cellulase with an enzyme activity of 200,000 U / g, pectinase with an enzyme activity of 30,000 U / g, galactosidase with an enzyme activity of 10,000 U / g, phytase with an enzyme activity of 8,000 U / g, and lipase with an enzyme activity of 250,000 U / g.

[0032] Embodiment 3:

[0033] The present embodiment provides a composite preparation for improving the digestibility of dried coconut shreds, including a composite enzyme preparation and an emulsifier, wherein the composite enzyme preparation includes xylanase with an enzyme activity of 8,000 U / g, glucanase with an enzyme activity of 9,000 U / g, mannanase with an enzyme activity of 15,000 U / g, cellulase with an enzyme activity of 200,000 U / g, pectinase with an enzyme activity of 30,000 U / g, galactosidase with an enzyme activity of 10,000 U / g, phytase with an enzyme activity of 8,000 U / g, and lipase with an enzyme activity of 250,000 U / g. The mass ratio of the emulsifier to the composite enzyme preparation is 1:9. In terms of mass percentage, the emulsifier includes 65% sucrose fatty acid ester and 35% quartz powder.

[0034] Embodiment 4:

[0035] In this example, the effect of the composite preparation on shredded coconut was studied by using the monogastric animal bionic digestive system, namely the chicken bionic digestive system.

[0036] Control group 1: no preparation was added, only the desiccated coconut shreds were subjected to bionic digestion;

[0037] Control group 2: lipase with an activity of 250,000 U / g was added to the dried coconut shreds at an addition amount of 300 g / t.

[0038] Experimental group 1: adding the composite preparation for improving the digestibility of shredded coconut provided in Example 1 to shredded coconut at an addition amount of 300 g / t;

[0039] Experimental group 2: The composite preparation for improving the digestibility of shredded coconut provided in Example 2 was added to shredded coconut at an addition amount of 300 g / t;

[0040] Experimental group 3: The composite preparation for improving the digestibility of shredded coconut provided in Example 3 was added to shredded coconut at an addition amount of 300 g / t.

[0041] Each treatment group had 5 parallels, and the results of chicken bionic digestion are shown in Table 2.

[0042] Table 2 Bionic digestibility of shredded coconut under chicken bionic digestive system (on a dry basis), %

[0043]

[0044] Compared with the control group, after adding the composite preparation provided by the present invention, the dry matter digestibility, fat digestibility, protein digestibility and fiber digestibility of the dried coconut shreds are all improved, especially when used in combination with lipase and emulsifier, the effect is better.

[0045] Embodiment 5:

[0046] The dried coconut shreds were crushed and passed through a 40-mesh sieve to obtain dried coconut shreds powder. The effect of the compound preparation on dried coconut shreds was studied through the chicken bionic digestive system, a monogastric animal bionic digestive system.

[0047] Control group 1: no preparation was added, only the desiccated coconut shreds were subjected to bionic digestion;

[0048] Control group 2: lipase with an activity of 250,000 U / g was added to the dried coconut shreds at an addition amount of 100 g / t.

[0049] Experimental group 1: The composite preparation for improving the digestibility of shredded coconut provided in Example 1 was added to shredded coconut at an addition amount of 100 g / t;

[0050] Experimental group 2: The composite preparation for improving the digestibility of shredded coconut provided in Example 2 was added to shredded coconut at an addition amount of 100 g / t;

[0051] Experimental group 3: The composite preparation for improving the digestibility of shredded coconut provided in Example 3 was added to shredded coconut at an addition amount of 100 g / t.

[0052] Each treatment group had 5 parallels, and the results of chicken bionic digestion are shown in Table 3.

[0053] Table 3 Bionic digestibility of shredded coconut under chicken bionic digestion system (on a dry basis), %

[0054]

[0055] The shredded coconut is crushed to improve the bionic digestibility. Compared with the control group, the dry matter digestibility, fat digestibility, protein digestibility and fiber digestibility of the shredded coconut are improved by adding the composite preparation provided by the present invention, especially the effect is better when used in combination with lipase and emulsifier.

[0056] Embodiment 6:

[0057] Weigh 1.0000 shredded coconut and 10 mL of pH 2.0 hydrochloric acid buffer in a 50 mL conical flask. According to the experimental design, add the corresponding emulsifier and lipase (enzyme activity is 250,000 U / g) respectively. Oscillate in a 42°C water bath shaker for 45 minutes. At this time, the pH of the entire digestion system is about 3.0. Adjust the pH with 1 L of 4% NaHCO3 and add pH 6.5 potassium hydroxide buffer. At this time, the pH of the entire digestion system is about 6.7. Continue to oscillate at 42°C for 4 hours. After the timing is over, quickly add 1 mlL of 95% ethanol solution to terminate the reaction. Add 0.3 mL of phenolphthalein reagent to the reaction solution, titrate with 0.1 mol / L sodium hydroxide standard solution, and record the volume of sodium hydroxide standard solution consumed. Counting number calculation and processing: Counting oleic acid value. Expressed as the number of milligrams of sodium hydroxide required to neutralize the free fatty acids in 1 g of emulsified oil, the calculation formula is:

[0058]

[0059] Wherein, V1: the volume of sodium hydroxide standard solution consumed when titrating the sample, in milliliters (mL);

[0060] V2: The volume of sodium hydroxide standard solution consumed when titrating the blank, in milliliters (mL):

[0061] c: concentration of sodium hydroxide standard solution, in moles per liter (mol / L);

[0062] M: molar mass of sodium hydroxide solution (g / mol);

[0063] m: weight of the sample (g).

[0064] The results are shown in Table 4.

[0065] Table 4 Effect of acid value on dry coconut oil

[0066]

[0067] Under the action of lipase, fat is hydrolyzed into free fatty acids, glycerol and glycerides, etc., for animal absorption and utilization. Therefore, the amount of free fatty acids can reflect the application effect of lipase to a certain extent. Oleic acid value refers to the content of free fatty acids in oils and fats. Oleic acid value increases with the increase of the amount of lipase added in the present invention. The combined effect of emulsifier and lipase in the present invention is better than that of adding them separately.

[0068] The above examples are merely illustrative of the present invention and do not constitute a limitation on the protection scope of the present invention. All designs that are the same or similar to the present invention fall within the protection scope of the present invention.

Claims

1. A composite preparation for improving the digestibility of dried coconut shreds, characterized in that: The invention comprises a composite enzyme preparation; the composite enzyme preparation comprises xylanase with an enzyme activity of 5,000-250,000 U / g, glucanase with an enzyme activity of 8,000-200,000 U / g, mannanase with an enzyme activity of 10,000-200,000 U / g, cellulase with an enzyme activity of 12,000-250,000 U / g, pectinase with an enzyme activity of 10,000-250,000 U / g, galactosidase with an enzyme activity of 10,000-300,000 U / g, and phytase with an enzyme activity of 8,000-200,000 U / g.

2. The composite formulation for improving the digestibility of dried coconut shreds as claimed in claim 1, wherein: Also included is a lipase with an enzyme activity of 100,000-300,000 U / g.

3. The composite formulation for improving the digestibility of dried coconut shreds as claimed in claim 2, characterized in that: It includes xylanase with an enzyme activity of 8,000 U / g, glucanase with an enzyme activity of 9,000 U / g, mannanase with an enzyme activity of 15,000 U / g, cellulase with an enzyme activity of 200,000 U / g, pectinase with an enzyme activity of 30,000 U / g, galactosidase with an enzyme activity of 10,000 U / g, phytase with an enzyme activity of 8,000 U / g, and lipase with an enzyme activity of 250,000 U / g.

4. The composite formulation for improving the digestibility of dried coconut shreds as claimed in claim 2, characterized in that: The emulsifier is also included; the added amount of the emulsifier is 10%.

5. The composite formulation for improving the digestibility of dried coconut shreds as claimed in claim 4, characterized in that: The emulsifier includes at least one of sucrose fatty acid ester and quartz powder.

6. The composite preparation for improving the digestibility of dried coconut shreds as claimed in claim 4, characterized in that: In terms of mass percentage, the emulsifier includes 65% sucrose fatty acid ester and 35% quartz powder.

7. A feed additive, characterized in that: include: Dried coconut shreds and the composite preparation according to any one of claims 1 to 6.

8. The feed additive according to claim 7, characterized in that: The addition amount of the composite preparation is 100-300 g / t of the mass of the dried coconut shreds.

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