124results about How to "Improve pest resistance" patented technology

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The invention discloses a selenium-rich nutrient solution for a pear. The nutrient solution comprises, by weight, 20 to 30 parts of magnesium sulfate, 5 to 15 parts of zinc sulfate, 10 to 15 parts of copper sulfate, 10 to 25 parts of iron sulfate, 10 to 25 parts of urea, 8 to 15 parts of potassium sulfate, 10 to 15 parts of potassium dihydrogen phosphate and 25 to 40 parts of sodium selenite. Through application of the nutrient solution provided by the invention, selenium content of the pear can be substantially increased, production of the pear is promoted, net weight of fruit is increased, resistance of the pear to insect attack is enhanced, and attack by diseases and insect pests is reduced.

The invention discloses an insect-proofing organic fertilizer which is prepared from the following raw materials in parts by weight: 200 to 230 parts of chicken manure, 30 to 34 parts of castor bean, 20 to 23 parts of garlic stems, 10 to 12 parts of radix sophorae flavescentis, 10 to 12 parts of broadleaf sawdust, 12 to 14 parts of bones, 2 to 3 parts of plant ash, 3 to 4 parts of bran, 4 to 5 parts of talcum powder, 20 to 23 parts of urea, 40 to 45 parts of calcium superphosphate, 2 to 3 parts of borax, 5 to 6 parts of zinc sulfate, 3 to 4 parts of manganese sulfate, 2 to 3 parts of manganese sulfate, 3 to 4 parts of ammonium molybdate, 1 to 2 parts of calcium carbonate powder, 2 to 3 parts of sodium bicarbonate, 3 to 4 parts of EM (Effective Microorganism) agent, 4 to 6 parts of coating agent and a proper amount of water. The insect-proofing organic fertilizer is rich in organic nutrition, namely that the diversification of nutrient elements is realized, so that the yield and quality of a plant are improved; the soil structure and the soil fertility are also improved to a certain extent through the added raw materials, so that an important significance for promoting agricultural mechanization and ecological food sharing is achieved. Thus, the insect-proofing organic fertilizer has a broad prospect. In addition, the insect-proofing capacity and disease-resisting capacity of a crop are improved through the addition of the castor bean, the garlic stems, the radix sophorae flavescentis and the like, so that the breeding of soil microorganisms is promoted; moreover, the pollution to the environment is avoided. Thus, the insect-proofing organic fertilizer has certain economic and environmental benefits.

The invention discloses a drug substrate fertilizer particle agent to prevent cotton seeding pest, which is characterized by the following: adopting nitrogen, phosphor, potassium or composite fertilizer as carrier; allocating imidacloprid, thiram, triazole ketone and adjuvant according to certain proportion; making the drug particle agent; modifying the nourishing condition and seeding growing environment; reinforcing pest-proof and growing property.

The invention discloses a corn planting method. The method comprises the steps of base fertilizer application, seed soaking and sowing, transplanting, field management, harvesting and the like, Chinese herbal liquid medicine is adopted for soaking seeds, and a variety of insecticide is adopted for cooperating in sterilization, so that young seedlings and fully-grown seedlings are great in disease resistance, soil is good in drainage capability and water retention capability, corn growth is facilitated, Chinese herbal medicine is utilized for replacing chemical insecticide, environmental pollution is reduced, corn plants which are planted and finally obtained are high in acre yield and excellent in quality, and economic benefits are remarkable.

The invention provides a pregermination method of rice seeds. The pregermination method includes: 1), seed drying, namely selecting the rice seeds good and full in quality, evenly spreading the rice seeds on concrete ground or a cushion and loading the rice seeds in a gunny bag after drying for 1-2 days; 2), seed soaking, namely soaking the seeds in clean water for 5-8 hours and then putting the seeds into a seed dressing agent for soaking for 30-40 minutes; 3), disinfection, namely adding 10kg of warm water to 8g of strong chlorine for mixing 3.5kg of the soaked rice seeds in proportion, performing disinfection for 3-5 hours and then fishing out the seeds to be cleaned in clean water and dried; 4), pregermination, namely filtering the rice seeds, evenly spreading the rice seeds on the bag, sealing the periphery of the bag with straws, controlling temperature to be from 38-42DEG C, and allowing the rice seeds to sprout after around 20 hours, wherein pregermination can be completed after 2-3 days. With the method, use of pesticides is reduced, labor force is saved, cost in pregermination is reduced, survival rate of rice is increased, disease resistance and insect resistance of the rice are enhanced, economic benefit is increased, and high promotion value is achieved.

The invention discloses a method for planting potatoes. During the germination period, water should be sprinkled to prevent dryness. The cutting tools used for cutting should be strictly sterilized with potassium permanganate or alcohol to prevent microorganisms and pathogenic bacteria from causing damage to potato tubers. Infection, the potato pieces after drying in the shade are moved to a warm and sunny place to dry the seeds, so that the young shoots are green and strong, and the foliar spraying of 1% potassium dihydrogen phosphate solution can promote the early emergence of seedlings, and the flower buds are removed during the flowering period, reducing Nutrient consumption can promote the development of potato pieces. When the potato plant height is 18 cm, spray the water with paclobutrazol, which can prevent the plants from growing excessively and lodging, and increase the rate of large and medium potatoes. After the middle flowering period, use carbendazim to prevent and control late blight , a potato planting method proposed by the present invention, which greatly saves labor, reduces the cost of potato planting, improves the survival rate of potatoes, enhances the benefits of planting potatoes, enhances the disease resistance and pest resistance of potatoes, and achieves The purpose of high yield and high quality has great promotion value.

The invention discloses a bacterial agent fermented organic fertilizer. The bacterial agent fermented organic fertilizer is prepared from the following raw materials in parts by weight: 80-100 parts of livestock manure, 10-12 parts of szechwan chinaberry fruit saw dust, 8-12 parts of weeds, 8-10 parts of quartz stone powder, 6-7 parts of humic acid, 6-8 parts of potassium humate, 6-8 parts of tea seed shell carbon particles, 6-8 parts of attapulgite powder, 4-5 parts of polyacrylamide, 4-6 parts of gypsum, 5-6 parts of soybean meal, 3-4 parts of an EM bacterial agent, 2-3 parts of sugarcane ash, 4-5 parts of a soil modifying agent and a proper amount of water. The fertilizer disclosed by the invention adopts a mixture of an organic fertilizer and an inorganic fertilizer, is more reasonable in nutrient elements, and can be used for rapidly promoting the growth and development of crops; the pest-resistant capacity of the crops can be significantly increased by virtue of part of added traditional Chinese medicine raw materials; by virtue of the attapulgite powder added in a formula, the fertilizer is not easy to cause an agglomeration phenomenon, and soil can be improved at the same time; the bacterial agent fermented organic fertilizer disclosed by the invention is beneficial for the growth of plants, and has good economic and social values.

The invention relates to potassium polyphosphate slow-release fertilizer for sugarcane, and a production method of the potassium polyphosphate slow-release fertilizer. The potassium polyphosphate slow-release fertilizer is prepared from the following raw materials in parts by weight: 45-60 parts of urea, 25-28 parts of potassium polyphosphate, 30-35 parts of biochemical potassium fulvate, 0.2-0.4 part of magnesium sulfate, 0.2-0.3 part of calcium chloride, 1-1.5 parts of trace elements, 2-5 parts of tea saponin, 8-10 parts of peanut bran, 5-10 parts of bone meal, 10-15 parts of rotten chicken manure and 10-15 parts of bentonite. The potassium polyphosphate slow-release fertilizer for the sugarcane is prepared according to the nutrient uptake features of sugarcane growth; the potassium polyphosphate slow-release fertilizer can provide multiple nutrient elements needed by the sugarcane growth; furthermore, the nutritional ingredients in the fertilizer can be slowly released, so that the fertilizer efficiency is lasting, and the utilization rate of the fertilizer is facilitated to be increased; the potassium polyphosphate slow-release fertilizer has the effects of increasing both production and income and increasing the sugar content of the sugarcane.

The invention discloses a method for improving the yield of sugar orange, and belongs to the technical field of agricultural planting. The method for improving the yield of the sugar orange includes the steps: 1 fertilization; 1.2 fruit fertilizer stabilization; 1.3 summer manuring; 1.4 fruit fertilizer collection; 2 spraying flower retention; 3 circumferential wiring; 4 tip control and drop prevention; 5 nourishing and fruit swelling. The method is improved from the aspects of fertilization, spraying flower retention, circumferential wiring fruit retention, tip control and drop prevention, nourishing and fruit swelling, the fruit quality of the sugar orange is greatly improved, and the yield is greatly improved.

The invention belongs to the technical field of agricultural crop fertilizer application, and particularly relates to a spray agent special for foliage vegetables. The spray agent special for foliagevegetables comprises a component A and a component B; the component A and the component B are mixed according to a weight ratio of 1:1, then are added into water with total weight of 200-300 times, and then are uniformly mixed, thus obtaining the spray agent special for foliage vegetables; the component A is mainly prepared by fermenting materials such as coffee grounds, peanut cakes, sasangua cake and zymocyte and then adding lemon pomace enzyme, a radix stemonae extracting solution and the like; the component A contains rich organic nitrogen, phosphorus and potassium elements, contains a great amount of micromolecule amino acid, polysaccharide, vitamin and mineral nutritive elements and substance such as beneficial bacteria; the component A is matched with substances such as bone meal, sweet tea and microelements in the component B, and therefore, the spray agent is complete in nutrition, and rich in various nutrients and medium and trace elements, can rapidly supplement nutrients required by vegetables, is good and lasting in fertilizer efficiency, and can effectively improve the yield and quality of vegetables.

The invention discloses a novel fusion insecticidal protein Cry1Am, and a coding gene and an application thereof. The fusion insecticidal protein has high insecticidal activity against wild type and Cry1Ab/Cry1Ac-resistant insects, and can be used for killing lepidopteran pests such as Asiatic corn borer, and for improving the insecticidal capacity of transgenic crops. The fusion insecticidal protein can effectively kill corn borers with Cry1Ac protein resistance, and can be used in insect resistance control.

The invention discloses a saline-alkali soil repairing microbial inoculum and a preparation method thereof. The microbial inoculum is obtained by adsorbing and embedding a functional microorganism fermentation solution and an acid-producing microorganism fermentation solution through an immobilized carrier, wherein the microorganism immobilized carrier comprises medical stones, citric acid, carrageenan and carboxyethyl cellulose; the functional microorganism fermentation solution is prepared by carrying out anaerobic fermentation on sulfate-reducing bacteria, denitrifying phosphate-accumulating bacteria, shewanella, pseudomonas fluorescens and bacillus licheniformis, which are screened through He-Ne laser induced screening; the acid-producing microorganism fermentation solution is obtainedby carrying out aerobic fermentation on gluconobater oxydans, rhizopus oryzae and aspergillus niger. According to the saline-alkali soil repairing microbial inoculum, the microorganism immobilized carrier is used for adsorbing and embedding the microorganism fermentation solution so that microorganisms are gradually diluted to saline-alkali soil and the tolerance of the microorganisms is improved; He-Ne laser induced breeding is utilized and the resistance capability of the microorganisms on bad factors is improved, so that the microorganism activity and the repairing capability are improved.

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45. The invention also includes the nucleotide sequence of SEQ ID NO:47 and 1-14, or a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:48 and 15-31, including biologically active variants and fragments thereof.

The invention discloses a long bean planting method. The long bean planting method comprises the steps that land preparation, base fertilizer application and bedding are performed, wherein soil is deeply ploughed for 30 cm, a base fertilizer for aiding is applied and contains 7000-8000 kg/mu of miscellaneous manure, wherein the miscellaneous manure is prepared from, by weight, 30-50 parts of plant ash, 50-80 parts of straw, 25-40 parts of chicken manure and 10-20 parts of chimney ash; field management is performed, wherein 30-50 kg/mu of fertilizer liquid is applied within 0.3-0.5 hour after each time of watering, the fertilizer liquid is prepared by evenly mixing 30-50 parts of weed, 30-40 parts of straw, 30-50 parts of chicken manure and 6-10 parts of chimney ash by weight, adding water and soaking the mixture at the temperature of 30-50 DEG C for 1-2 hours. The operating parameters of key links for long bean planting are optimized, so that the planting process is scientific and reasonable, the long beam yield is increased, the long beam quality is improved, and the high-yield and high-quality purpose is achieved.

The invention relates to biological organic fertilizer taking livestock excrements as raw materials. The biological organic fertilizer comprises the following raw materials in parts by weight: 80 to 100 parts of a microbial fermentation composition, 15 to 20 parts of urea, 5 to 10 parts of calcium hydrogen phosphate and 3 to 5 parts of potassium chloride, wherein the microbial fermentation composition is obtained by performing medium-temperature aerobic composting treatment on a microbial mixed bacterial agent, a combined enzyme agent, an organic raw material and water according to the weightratio of (1-1.5):(0.5-1):100:(10-20) at 25 to 50 DEG C; the microbial mixed bacterial agent comprises bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, bacillus cereus, bacillus licheniformis, aspergillus niger, aspergillus oryzae and bacillus thuringiensis; the combined enzyme agent is a mixture of protease, cellulase, glucanase, pectinase and lipase; and the organic material is amixture of livestock excrements and straws. According to the design, stink and damage to soil are not liable to occur, and the composting period is short.

The invention discloses a biological organic fertilizer. The biological organic fertilizer comprises the following materials in parts by weight: 80-100 parts of a microorganism-fermented composition and 5-10 parts of urea, wherein the microorganism-fermented composition is obtained by carrying out medium-temperature aerobic composting treatment on a microorganism mixed agent, a combined enzyme agent, an organic material and water in a weight ratio of 1-1.5:0.5-1:100:10-20 at a temperature of 25-50 DEG C; the microorganism mixed agent comprises the materials of: bacillus subtilis, bacillus megaterium, bacillus mucilaginosus, bacillus cereus, bacillus licheniformis, aspergillus niger, aspergillus oryzae and bacillus thuringiensis; the combined enzyme agent is a mixture of protease, cellulase, glucanase, pectinase and lipase; the organic material is a mixture of kitchen waste and straws. The biological organic fertilizer disclosed by the invention not only is not likely to generate odourand damage soil, but also is short in composting period.

A method for enhancing insect feeding resistance of plants through beta-ocimene includes the steps that under the situation that the growing plants and the beta-ocimene are in a relatively closed space or in an open space where air does not circulate or flows weakly, the plants make physical contact with the beta-ocimene for over 2 hours, and the final concentration of the beta-ocimene in the space is larger than or equal to 0.1 umol/L. Pests do not eat or seldomly eat the plants processed by the beta-ocimene, but the plants not processed by the beta-ocimene are seriously damaged. Thus, the beta-ocimene can effectively the insect pest resistance of the plants. By means of the method, a novel method is provided for controlling damage of the pests to crops, the defects of a current pesticide and biological insect pest prevention method are overcome, and a new environment-friendly, non-toxic, harmless and high-efficiency insect pest prevention means is created.

The invention relates to a preparation method of biological organic fertilizer taking livestock excrements as raw materials. The preparation method comprises the following steps: mixing a microbial mixed bacterial agent, a combined enzyme agent, an organic raw material and water uniformly according to the ratio of (1-1.5):(0.5-1):100:(10-20), adjusting C/N and initial water content, performing aerobic fermentation at 25 to 50 DEG C for 7 to 10 days to obtain a microbial fermentation composition, and mixing the microbial fermentation composition, urea, calcium hydrogen phosphate and potassium chloride uniformly according to the ratio of (80-100):(15-20):(5-10), wherein the microbial mixed bacterial agent comprises bacillus subtilis, bacillus megatherium, bacillus mucilaginosus, bacillus cereus, bacillus licheniformis, aspergillus niger, aspergillus oryzae and bacillus thuringiensis; the combined enzyme agent is a mixture of protease, cellulase, glucanase, pectinase and lipase; and the organic material is a mixture of livestock excrements and straws. According to the design, stink and damage to soil are not liable to occur, and the composting period is short.

The invention discloses a date tree germination-accelerating and seedling-culturing technology suitable for an arid region. The planting technology is realized by adopting the following steps: (1) selecting uniform and full dates without plant diseases and insect pests, dewatering the dates until the water content is less than 15% and storing the dates in a sealing manner; (2) pre-processing the dates; (3) performing germination acceleration treatment to enable the germination rate of seeds to be above 85%; (4) culturing seedlings artificially; (5) transplanting and field-planting; (6) performing growth management, namely, irrigating every 2-3 days half a month after the transplantation, after seedling survival, irrigating every 10-15 days during the period from the last ten days of March to middle ten days of May, spreading 5 kg urea per each mu by combining with water irrigation after the end of May, and dressing diamine 10 kg per each mu by combining with intertillage and weeding after the second water irrigation for fields with relatively low soil fertility.