Method for enhancing insect feeding resistance of plants through beta-ocimene

A technology of ocimene and plants, which is applied in the field of enhancing the ability of plants to resist pests and feed, and can solve the problems of environmental ecology and human health, high cost and difficult to promote in large areas.

Active Publication Date: 2015-08-12
刘春林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above two methods have their own advantages and disadvantages: the biggest advantage of chemical pesticide control is high efficiency and low cost, and is less affected by the environment. The disadvantage is that it is extremely harmful to the environment and human health; Friendly, its shortcomings are also obvious, that is, the effect of biological control is greatly affected by environmental conditions, and is also related to the developmental period of pests; in addition, the high cost is not easy to promote in large areas
After the applicant treated the plants with β-ocimene, removed the β-ocimene and contacted the plants, and found that the plants themselves can resist the feeding of insect pests. This discovery makes it possible to use no pesticides in agricultural production. Moreover, this It has been found that no literature or patent reports have been found so far

Method used

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  • Method for enhancing insect feeding resistance of plants through beta-ocimene
  • Method for enhancing insect feeding resistance of plants through beta-ocimene
  • Method for enhancing insect feeding resistance of plants through beta-ocimene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Effect of β-Ocimene Treatment on Spodoptera litura Larvae Feeding Arabidopsis

[0018] Two plants of Arabidopsis thaliana that had grown for 20 days were placed in two cleaned and air-dried desiccators; β-ocimene was added to one of the desiccators so that the final concentration of β-ocimene in the desiccator was 5 μmol / L, and quickly cover the lid, as a treatment group; do not add any substance in another desiccator, as a blank control. The two desiccators are sealed, and then placed in a plant growth chamber with a temperature of 20-25° C., an illumination of 8000-10000 Lux, and a relative humidity of 60-75%. After 16 hours, the plants were taken out, and the two plants were put into a fume hood to remove β-ocimene in the air. After 10 minutes, they were transferred to the growth room and placed side by side. Each plant was inoculated with 5 second instar Spodoptera litura larvae. The results were observed overnight.

[0019] The results showed that the ...

Embodiment 2

[0020] Example 2 Effect of β-ocimene treatment on tomato eating by Tetranychus urticae

[0021] Surround a circle of about one square meter on the ground with an agricultural film, put 5 potted tomato potted plants growing for 25 days in the circle, and the film circle is 10 centimeters higher than the plant; put three glass slides on the ground in the circle, Arrange in an equilateral triangle, drop 100 μL of β-ocimene on each slide, and let it volatilize freely. After 8 hours, the final concentration of β-ocimene in the film circle was measured to be greater than 0.5 μmol / L, and the plant was transferred to the fume hood to remove the β-ocimene in the air. After 10 minutes, the temperature was changed to 20-25°C. ℃, light is 8000-10000Lux, relative humidity is 60-75% in the plant growth room. Select 5 leaves from the plants treated with β-ocimene and those without β-ocimene treatment, inoculate 15 Tetranychus urticae on each leaf, and check the degree of damage after 24 hou...

Embodiment 3

[0023] Example 3 Effects of β-Ocimene Treatment on Rapeseed Rapeseed by Pieris rapae Larvae

[0024] In an artificial climate box of 2 cubic meters, place 3 potted rape plants that have grown for 40 days; add β-ocimene in the artificial climate box, and move the artificial climate box to a temperature of 20-25 ° C and a light of 8000 -10000Lux, in a plant growth chamber with a relative humidity of 60-75%. After 5 hours, the final concentration of β-ocimene in the artificial climate box was 2.5 μmol / L, take out the plants, put the two plants in a fume hood to remove β-ocimene in the air, and transfer them to the growth room after 10 minutes , placed in the same growth chamber as untreated plants, each plant was inoculated with 5 cabbage larvae, and the results were observed after 24 hours.

[0025] The results showed that almost no food was eaten by Pieris rapae larvae on the plants treated with β-ocimene (see image 3 B), while the leaves of the plants treated with β-ocimene...

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Abstract

A method for enhancing insect feeding resistance of plants through beta-ocimene includes the steps that under the situation that the growing plants and the beta-ocimene are in a relatively closed space or in an open space where air does not circulate or flows weakly, the plants make physical contact with the beta-ocimene for over 2 hours, and the final concentration of the beta-ocimene in the space is larger than or equal to 0.1 umol/L. Pests do not eat or seldomly eat the plants processed by the beta-ocimene, but the plants not processed by the beta-ocimene are seriously damaged. Thus, the beta-ocimene can effectively the insect pest resistance of the plants. By means of the method, a novel method is provided for controlling damage of the pests to crops, the defects of a current pesticide and biological insect pest prevention method are overcome, and a new environment-friendly, non-toxic, harmless and high-efficiency insect pest prevention means is created.

Description

technical field [0001] The invention relates to a new method for improving plant resistance to pests, in particular to a new method for treating plants with β-ocimene, thereby enhancing the ability of plants to resist pest feeding. Background technique [0002] At present, there are mainly two methods for the prevention and control of plant pests: chemical pesticide methods and biological control. Among them, the chemical pesticide method is mainly to kill the pests by synthesizing toxic substances that have a fatal effect on the pests through chemical synthesis; the insecticidal pesticides used in this method are obviously harmful to the ecological environment and human health, such as DDT that has been taken in production will still remain in the ecosystem after a hundred years. With the deepening of people's understanding of the hazards of pesticides, people pay more and more attention to biological control. Biological control is a method that one or one type of organism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01G7/06A01N27/00A01P7/02A01P7/04
CPCA01G7/06A01G13/00A01N27/00
Inventor 刘春林
Owner 刘春林
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