Saline-alkali soil repairing microbial inoculum and preparation method thereof

A microbial agent and saline-alkali soil technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of general improvement effect, inability to reduce the alkalinity of saline-alkali land, and aggravate the degree of salinization, and achieve Improve the emergence rate and pest resistance, reduce the content of soluble inorganic salts, reduce the effect of salt content and PH value

Inactive Publication Date: 2018-10-19
汤永红
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the improvement methods of saline-alkali soil mainly include physical irrigation and salt washing, chemical neutralization, microbial improvement and other forms. Among them, physical irrigation and water washing of salt only disperses the salt in saline-alkali soil to other areas. It is slow and consumes a lot of manpower and material resources. Chemical neutralization is to use acidic reagents to reduce the pH value of saline-alkali land, but it is easy to cause soil compaction and destroy microbial flora. The improvement of microbial bacterial agents can not only promote the decomposition of soil organic matter and crop metabolism, but also reduce the obstacles of repeated cropping. And it can degrade chemical fertilizers and pesticide residues, improve crop quality and yield, but the anti-osmotic pressure ability of microorganisms is generally low, and the mortality rate is high in high-concentration saline-alkali soi

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  • Saline-alkali soil repairing microbial inoculum and preparation method thereof
  • Saline-alkali soil repairing microbial inoculum and preparation method thereof
  • Saline-alkali soil repairing microbial inoculum and preparation method thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0027] Example 1

[0028] The weight percentage of each component of the saline-alkali soil remediation microbial inoculum: 25% of the microbial immobilized carrier, 55% of the functional microbial fermentation broth, and 20% of the acid-producing microbial fermentation broth.

[0029] The weight ratio of each component of the above immobilized carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose = 1:0.2:0.7:1.6.

[0030] The concentration of desulfurization sarcoid in the above-mentioned functional microorganism fermentation broth is higher than 5×10 7 CUF / ml, the concentration of denitrifying phosphorus accumulating bacteria is higher than 4×10 9 CUF / ml, Shewanella concentration higher than 2×10 9 CUF / ml, the concentration of C. fluorescens is higher than 6×10 8 CUF / ml, Bacillus licheniformis concentration higher than 8×10 7 CUF / ml.

[0031] The concentration of Acidobacter oxidans in the fermentation broth of the above acid-producing microorganisms ...

Example Embodiment

[0039] Example 2

[0040] The weight percentage of each component of the saline-alkali soil remediation microbial inoculum: 30% of the microbial immobilized carrier, 45% of the functional microbial fermentation broth, and 25% of the acid-producing microbial fermentation broth.

[0041] The weight ratio of each component of the above-mentioned immobilized carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose=1:0.25:0.75:1.8.

[0042] The above-mentioned sulfate-reducing bacteria are Desulfomonas.

[0043] The pH value of the above-mentioned acid-producing microorganism fermentation broth is 3.5.

[0044] The preparation method of the above-mentioned saline-alkali soil remediation microbial inoculum is the same as Example 1, and the difference is:

[0045] The composition difference of the medium prepared by S1 and S2 is shown in Table 1:

[0046] Table 1

[0047]

[0048] S3: Maifan stone crushed particle size is 60 mesh; S4: He-Ne laser current intens...

Example Embodiment

[0049] Example 3

[0050] The weight percentage of each component of the saline-alkali soil remediation microbial inoculum: 35% of the microbial immobilized carrier, 35% of the functional microbial fermentation broth, and 30% of the acid-producing microbial fermentation broth.

[0051] The weight ratio of each component of the above immobilized carrier: medical stone: citric acid: carrageenan: carboxyethyl cellulose = 1:0.3:0.8:2.

[0052] The above-mentioned sulfate-reducing bacteria are desulfurizing nematodes.

[0053] The pH value of the above-mentioned acid-producing microorganism fermentation broth is 4.5.

[0054] The preparation method of the above-mentioned saline-alkali soil remediation microbial inoculum is the same as Example 1, and the difference is:

[0055] The composition difference of the medium prepared by S1 and S2 is shown in Table 2:

[0056] Table 2

[0057]

[0058] S3: Maifan stone crushed particle size is 80 mesh; S4: He-Ne laser current intensi...

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Abstract

The invention discloses a saline-alkali soil repairing microbial inoculum and a preparation method thereof. The microbial inoculum is obtained by adsorbing and embedding a functional microorganism fermentation solution and an acid-producing microorganism fermentation solution through an immobilized carrier, wherein the microorganism immobilized carrier comprises medical stones, citric acid, carrageenan and carboxyethyl cellulose; the functional microorganism fermentation solution is prepared by carrying out anaerobic fermentation on sulfate-reducing bacteria, denitrifying phosphate-accumulating bacteria, shewanella, pseudomonas fluorescens and bacillus licheniformis, which are screened through He-Ne laser induced screening; the acid-producing microorganism fermentation solution is obtainedby carrying out aerobic fermentation on gluconobater oxydans, rhizopus oryzae and aspergillus niger. According to the saline-alkali soil repairing microbial inoculum, the microorganism immobilized carrier is used for adsorbing and embedding the microorganism fermentation solution so that microorganisms are gradually diluted to saline-alkali soil and the tolerance of the microorganisms is improved; He-Ne laser induced breeding is utilized and the resistance capability of the microorganisms on bad factors is improved, so that the microorganism activity and the repairing capability are improved.

Description

technical field [0001] The invention relates to the technical field of saline-alkali land improvement, in particular to a saline-alkali soil restoration microbial agent and a preparation method thereof. Background technique [0002] Due to the redistribution of various soluble salts in the horizontal and vertical directions on the surface, the salt is continuously accumulated on the soil surface in the salt-collecting area, forming saline-alkali soil that endangers the growth of crops. According to incomplete statistics from UNESCO and the Food and Agriculture Organization, the area of ​​saline-alkali land in China is about 99.13 million hectares, and the degree of salinization is generally high, making it difficult for crops to survive. At present, the improvement methods of saline-alkali soil mainly include physical irrigation and salt washing, chemical neutralization, microbial improvement and other forms. Among them, physical irrigation and water washing of salt only dis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N11/14C12N11/02C12N11/12C09K17/40C09K109/00C12R1/01C12R1/10C12R1/845C12R1/685
CPCC09K17/40C12N1/14C12N1/20C12N11/02C12N11/12C12N11/14
Inventor 汤永红
Owner 汤永红
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