Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid

A cement-based material and microbial repairing technology, which is applied to the repairing method of cement-based material cracks and the culture solution and repairing nutrient solution, uses microorganisms to deposit calcium carbonate to repair the field of cement-based material cracks, and can solve problems that affect the normal use of materials and induce macroscopic effects. Cracks, limiting the scope of application, etc., to achieve the effects of good volume stability and durability, low cost, and outstanding environmental friendliness

Active Publication Date: 2008-11-12
SOUTHEAST UNIV
View PDF0 Cites 37 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if it cannot be repaired in time, it will not only affect the normal use of the material, but may also induce macroscopic cracks and brittle fracture.
At present, the crack repair materials commonly used in engineering mainly include cement-based permeable crystalline materials, polymer mortar, polyme

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid
  • Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid
  • Method for recovering cement-based material crack by means of microorganism, culture fluid and repair nutrient fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Utilize microorganism to repair the crack method of cement-based material

Embodiment 1-1

[0032]Example 1-1 A method of using microorganisms to repair cracks in cement-based materials, inoculating the bacterial strain Bacillus pasteurii on a medium containing a urea-containing substrate, performing shaking culture at 25°C, and taking out the culture after 16 hours Centrifuge the bacterial solution at 5000rpm for 5min, remove the supernatant, collect the cells of the strain with the culture medium, measure the OD value of the transmittance of the concentrated bacterial solution with a UV-visible light spectrophotometer, and collect it according to the linear calibration curve between the OD value and the concentration of the bacterial solution. The cell concentration of the strain was controlled at 2×10 9 within the range, then add 8 standard sand, urea, and Ca(NO 3 ) 2 4H 2 O mixture, mix it and mix it into a slurry, inject it into cement stone cracks with a width of 1 mm and a depth of 50 mm, inject 1 repairing nutrient solution into the cracks of cement stone e...

Embodiment 1-2

[0033] Embodiment 1-2 A method for repairing cracks in cement-based materials by using microorganisms, which is characterized in that the bacterial strain Bacillus pasteurii is inoculated onto a medium containing a urea-containing substrate, and cultured with shaking at 37°C for 24 hours Afterwards, take out the cultured bacteria solution, centrifuge at 8000rpm for 8min, remove the supernatant, collect the strain cells with the culture solution, measure the OD value of the light transmittance of the concentrated bacteria solution with a UV-visible light spectrophotometer, according to the linear calibration curve between the OD value and the concentration of the bacteria solution Control the cell concentration of the collected strains at 2×10 11 within the range of cell / mL, then add 12 g of standard sand, urea, and Ca(NO 3 ) 2 4H 2 O mixture, mix it and mix it into a slurry, inject it into cement stone cracks with a width of 5 mm and a depth of 1 mm, inject 4 mL of repairing...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for repairing cement-based material cracks, as well as a culture solution and a repair nutrient solution. The method for repairing cement-based material cracks by through microorganisms comprises the following steps that: a Bacillus pasteurii strain is inoculate onto a culture medium provided with a urea-containing substrate; shake cultivation is carried out at a temperature of between 25 and 37 DEG C, and then a culture bacteria solution is taken out and centrifuged and has supernatant fluid removed; strain cells are collected through the culture solution; the concentration of the strain cells is controlled in a range of between 2x10<9> and 1x10<11> cell/ml; standard sand, urea and Ca(NO3)2.4H2O mixture are added to each milliliter of strain cell solution obtained through collection, mixed, stirred into slurry and injected into cement stone cracks; the frequency of the repair nutrient solution injection is not less than two times; finally, maintenance is carried out. In the culture solution, each liter of culture solution contains 4 to 6 g of peptone, 2 to 4 g of beef extract and 20 to 60 g of urea. The method fully utilizes microbial resources in nature; CO3<2-> decomposed out through microbial enzyme can chelate Ca<2+> in a substrate so as to be mineralized and deposit calcium carbonate, and is close in the combination with the substrate and good in stability.

Description

technical field [0001] The invention relates to a method for repairing cracks in cement-based materials, a culture solution and a repairing nutrient solution, in particular to a method for repairing cracks in cement-based materials by using microorganisms to deposit calcium carbonate, as well as a culture solution and a repairing nutrient solution. Background technique [0002] Cement-based materials are currently the most widely used building materials, and their biggest disadvantage is that they are prone to micro-cracks and local damage under the influence of the surrounding environment during the material preparation process and service period. Cracks in concrete are inevitable, and their microscopic cracks are determined by their own physical and mechanical properties. However, if it cannot be repaired in time, it will not only affect the normal use of the material, but also may induce macroscopic cracks and brittle fracture. At present, the crack repair materials comm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12P3/00E04G23/02C12R1/07
Inventor 钱春香王瑞兴
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap