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Method for preparing ginseng rare saponin by virtue of microorganism enzymatic conversion of ginsenosid Re

A technology of ginseng rare saponins and ginseng stem and leaf saponins, which is applied in the field of preparation of ginseng rare saponins, can solve the problems that there is no method for preparing ginseng rare saponins, and achieve the effect of low cost and simple process

Active Publication Date: 2015-09-09
三蚁科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no method for preparing rare ginsenosides such as C-Mx, C-Mc, and C-Y has been proposed so far.

Method used

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  • Method for preparing ginseng rare saponin by virtue of microorganism enzymatic conversion of ginsenosid Re

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Rare ginseng saponins C-Mx, C-Mc, C-Y, C-K are prepared by enzymatic conversion of Panax notoginseng stem and leaf saponins (mainly containing Rb3, C-Mx1 and Rc, Rb2 saponins; also a small amount of Rb1 and Rd saponins).

[0020] 1) Preparation of enzyme: Aspergillus niger ( Aspergillus niger ) Bacterial enzyme fermentation preparation is carried out by a common method. Aspergillus niger ( Aspergillus niger g.48 bacteria, isolated from Daqu, Literature: Liu Chunying, etc. Process Biochemistry 2014. 49, 813-820, Dalian University of Technology has been preserved and made publicly available) inoculated in a medium containing 5% wort and 0.03% of Panax notoginseng stem and leaf saponins, cultured at 28~32℃, ventilated and stirred for 4~ After 5 days, centrifuge to remove the residue; add 75% saturated ammonium sulfate powder to the supernatant, precipitate the enzyme protein overnight at 4°C, and collect the enzyme protein precipitate; dialyzed with 0.01 molar, pH 5.0 acet...

Embodiment 2

[0030] Commercially available ginseng stem and leaf glycol saponins (mainly containing Rc, Rb2, Rd saponins, and a small amount of Rb1 saponins) are enzymatically converted to prepare rare saponins C-Mc, C-Y, C-K.

[0031] 1) Preparation of enzyme: Aspergillus oryzae ( Aspergillus oryzae sp.39g, isolated from Daqu, Literature: Wang Dongming, Yu Hongshan, etc. Process Biochemistry 2012, 47, 133-138, Dalian University of Technology has been preserved and made publicly available) inoculated into a koji box containing 1 kg of wheat bran (containing 50 g of ginseng stem and leaf powder) with 50% sterilized water content. Incubate at ~32℃ for 4~7 days (stirring 4~6 times a day), then use 5 liters of 0.02 mol, pH 5.0 acetate buffer to extract the enzyme; centrifuge the enzyme solution, and use ammonium sulfate solid precipitation enzyme in the supernatant Protein; centrifuge to collect the precipitate, and dissolve it in 1000 ml of 0.02 molar, pH 5.0 acetate buffer; that is, the crude...

Embodiment 3

[0037] The commercially available American ginseng stem and leaf glycol saponins (mainly containing Rc, Rb3, and Rd saponins) are transformed with enzymes to prepare rare saponins C-Mc, C-Mx, and C-K.

[0038] 1) Preparation of enzyme: Aspergillus niger ( Aspergillus niger g.48 bacteria, isolated from Daqu, Literature: Liu Chunying, etc. Process Biochemistry 2014. 49, 813-820, Dalian University of Technology has been preserved and made publicly available), inoculated into a curved box containing 1 kg of wheat bran (containing 100 locust pollen) containing 50% sterilized moisture, and the temperature is 28~32 Cultivate for 6 to 8 days (stirring 4 to 6 times a day); then use 5 liters of 0.02 mol, pH 5.0 acetate buffer to extract the enzyme, collect the enzyme solution, centrifuge the enzyme solution to obtain the supernatant; reduce the supernatant Concentrate to one-fifth of the volume, and precipitate the enzyme protein with methanol; collect the precipitate by centrifugation a...

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Abstract

The invention discloses a method for preparing ginseng rare saponin by virtue of microorganism enzymatic conversion of ginsenosid Re. According to the method, aspergillus microorganisms are fermented to obtain an enzyme solution, and then the enzyme solution reacts with cheap ginseng with high contents of ginseng saponin Rb3, Rc, Rb2, Rd and the like or western stem and leaf glycol saponin or pseudo-ginseng steam and leaf saponin, so that a high-activity ginseng rare saponin C-Mx, C-Mc, C-Y, C-K mixture and monomers thereof are prepared. The method is low in cost, simple in process and free from pollution; products can be applied to ginseng products, functional foods and cosmetics.

Description

technical field [0001] The invention relates to a preparation method of rare ginseng saponins, in particular to a crude enzyme fermented by Aspergillus fungus to convert ginsenosides with high content of ginsenosides Rb3, Rc, Rb2, Rd, etc., to prepare highly active ginseng Methods for rare saponins C-Mx, C-Mc, C-Y, C-K, etc. Background technique [0002] Ginseng has a long history and has good tonic and therapeutic functions. The ginseng with the highest output is mainly ginseng ( Panax ginseng C.A.Mayer), American ginseng ( P. quinguefolus L.) and Panax notoginseng ( P. notoginseng (burk)). The main active ingredients of ginseng are ginsenosides (Ginsenoside, ginseng saponin), mainly triterpenoid tetracyclic triterpene dammarane type and pentacyclic triterpene oleanolic acid saponin, of which tetracyclic triterpene dammarane type saponin Mainly. Tetracyclic triterpene dammarane saponins are divided into protopanaxadiol type ginsenoside (PPD) and protopanaxatriol t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12R1/685C12R1/69
Inventor 鱼红闪刘春莹金凤燮
Owner 三蚁科技(广州)有限公司
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