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Preparation of compound enzyme rich in acidic pectinase as well as strain and application of compound enzyme

A technology of acid pectinase and compound enzyme, which is applied in the field of compound enzyme, can solve the problem of single enzyme system, achieve the effect of reducing feed-to-egg ratio, increasing egg production rate, and good application effect

Active Publication Date: 2020-08-18
HANGZHOU BIOCOM BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes the preparation of a compound enzyme rich in acid pectinase and its bacterial strain and application, which solves the problem of single enzyme system in the fermentation product in the prior art question

Method used

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  • Preparation of compound enzyme rich in acidic pectinase as well as strain and application of compound enzyme
  • Preparation of compound enzyme rich in acidic pectinase as well as strain and application of compound enzyme
  • Preparation of compound enzyme rich in acidic pectinase as well as strain and application of compound enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A preparation of a compound enzyme rich in acid pectinase, the specific preparation method is as follows:

[0047] 1 slant culture

[0048] Streak inoculation of Aspergillus niger BAK200345 on the slanted solid medium of the sterilized primary test tube, and culture at 25-35°C for 4-8d. Its culture medium is Cha's medium: 3% sucrose, 0.3% sodium nitrate, 0.1% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% potassium chloride, 0.001% ferrous sulfate, 1.2-1.8% agar powder, 121 Sterilize at ℃ for 20min.

[0049] 2 shake flask cultures

[0050] Scrape the first-stage test tube slant seeds obtained in step 1 of the 1-2 rings and culture them in the liquid medium of the second-stage shake flask, and the liquid volume of the shake flask is 100-150mL / bottle. Cultivate for 2-6 days at a temperature of 25-35° C. and a shaker rotation speed of 160-200 rpm to obtain a secondary shake flask seed liquid. The formula of the culture medium is: bran 2-6%, peptone 1-5%...

Embodiment 2

[0061] The slant medium in embodiment 1 is replaced with the following medium;

[0062] Glucose 2%, potato juice 20%, agar powder 1.2-1.8%, sterilized at 121°C for 20min.

[0063] or,

[0064] Bran juice 2-5%, beef extract 1-3%, peptone 0.5%-2%, dipotassium hydrogen phosphate 0.1-0.6%, magnesium sulfate 0.2-0.4%, agar powder 1.2-1.8%, sterilized at 121°C for 20min .

[0065] The rest of the conditions remain unchanged.

[0066] The solid medium in embodiment 1 is replaced with the following medium;

[0067] Bran 80-85%, soybean meal 5-10%, corn flour 5-10%, ammonium chloride 1-2%, magnesium sulfate 0.05%-0.1%, calcium carbonate 0.5%-1%, dipotassium hydrogen phosphate 0.2% -0.3%, initial moisture 60%-65%, sterilized at 121°C for 40min.

[0068] or,

[0069] Bran 80-90%, soybean meal 5-10%, palm meal powder 5-10%, ammonium sulfate 1-2%, magnesium sulfate 0.05%-0.1%, manganese sulfate 0.01-0.02%, calcium carbonate 1%-2% , Dipotassium hydrogen phosphate 0.2%-0.3%, initial m...

Embodiment 3

[0073] A preparation of a compound enzyme rich in acid pectinase, the specific preparation method is as follows:

[0074] 1 slant culture

[0075] Streak inoculation of Aspergillus niger strain CGMCC No.19614 on the slanted solid medium of the sterilized primary test tube, and culture at 25-35°C for 6-10d. Its culture medium is Cha's medium: 3% sucrose, 0.3% sodium nitrate, 0.1% dipotassium hydrogen phosphate, 0.05% magnesium sulfate, 0.05% potassium chloride, 0.001% ferrous sulfate, 1.2-1.8% agar powder, 121 Sterilize at ℃ for 20min.

[0076] 2 Erlenmeyer flask culture

[0077] In the ultra-clean workbench, scrape the slant spores of the primary test tube cultivated in step 1 into a glass bead bottle filled with 150-200mL sterile water (containing 100-500μL Tween-80), shake and mix well to make the spores fully Suspended in sterile water. Then inoculate 1-3mL / bottle of solid seeds on the solid medium of the secondary Erlenmeyer flask for cultivation. Cultivate at 28-35°C...

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Abstract

The invention provides preparation of a compound enzyme rich in acidic pectinase as well as a strain and application of the compound enzyme. A cultured fermentation product contains pectinase, xylanase, mannase, acid protease, cellulase, amylase and beta-glucanase. Aspergillus niger is named as aspergillus niger BAK200345, and the preservation number of the aspergillus niger is CGMCC No.19614. Thecompound enzyme rich in acidic pectinase provided by the invention is rich in pectinase types, and pectinesterase, pectate hydrolase, pectate lyase, polygalacturonase and rhamnogalacturonase exist, so that the compound enzyme is a complete pectinase system. The enzyme activity of the acidic pectinase is relatively high and can reach 132960 U / g at most; the compound enzyme can reduce the feed-eggratio, increase the laying rate of laying hens, reduce the excrement sticking phenomenon and improve the intestinal health, and is good in application effect.

Description

technical field [0001] The invention relates to the field of compound enzymes, in particular to the preparation of a compound enzyme rich in acid pectinase, its bacterial strain and its application. Background technique [0002] Pectin is a kind of polymer polysaccharide compound, which widely exists in fruits and vegetables, corn, soybean and other plants. It is the main component of plant cell wall, and it is mixed in the primary cell wall and intercellular space of plants. At present, most feed ingredients are plant ingredients, and pectin exists in plant feed ingredients as a kind of anti-nutritional factor. After entering the digestive tract, it increases the viscosity of chyme and affects the digestion and absorption of nutrients by animals. [0003] Pectin is mainly divided into three categories: pectin, pectic acid, and protopectin. Pectin is a long-chain polymer compound formed by linking galacturonic acid ester and a small amount of galacturonic acid through α-1,4...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/04C12N9/08C12N9/18C12N9/24C12N9/26C12N9/42C12N9/62C12N1/14A23K10/12A23K20/189A23K10/30A23K10/37A23K20/26C12R1/685
CPCC12N9/2437C12N9/248C12N9/2408C12N9/18C12N9/2414C12N9/2482C12N9/62C12N9/2402C12N9/0065C12N9/0006C12N9/2411C12N9/88C12N9/2488C12N9/2445C12Y302/01004C12Y302/01091C12Y302/01021C12Y301/01011C12Y302/01015C12Y402/02002C12Y302/01001C12Y302/01082C12Y302/01023C12Y302/01089C12Y302/01008C12Y302/01055C12Y101/01307C12Y302/01022C12Y302/01051C12Y302/01003C12Y302/0102C12Y302/01039C12Y302/01078C12Y302/01113C12Y101/01017C12Y304/21062C12Y111/01006A23K10/12A23K20/189A23K10/30A23K10/37A23K20/26C12R2001/685C12N1/145Y02P60/87
Inventor 王云龙吴勃徐永雷王天珍刘广晓
Owner HANGZHOU BIOCOM BIOLOGICAL TECH
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