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Novel pectate lyase and method of use for bio-scouring

A technology of Bacillus subtilis and pectic acid, applied in textiles and papermaking, biochemical treatment of enzymes/microbes, lyase, etc., can solve the problem of not being able to remove fiber-pigment groups

Inactive Publication Date: 2009-08-26
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Removing these pigments can be very difficult as detergents do little to remove the fibrous-pigment clusters from the surface to be cleaned

Method used

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  • Novel pectate lyase and method of use for bio-scouring
  • Novel pectate lyase and method of use for bio-scouring
  • Novel pectate lyase and method of use for bio-scouring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0401] Example 1. Synthesis and purification of enzyme

[0402] Using the aprE promoter for efficiently transcribing genes and the AprE signal sequence that enables the efficient secretion of pectate lyase into the medium, pectate lyase derived from a derivative of Bacillus subtilis subtilis strain 168 is produced in Bacillus subtilis. Using forward oligonucleotide primer 5'-TTCAGCGAAGACAGCGCGCAGGCAGCTGATTTAGGCCACCAGACGTTGGG-3' (SEQ ID NO: 3) and reverse primer 5'-TTTTCAAAGCTTTAATTTAATTTACCCGCACCCGCTTGATTT-3' (SEQ ID NO: 4), using PCR from chromosomal DNA (using standard phenol The extraction technique was isolated from the derivative of Bacillus subtilis subtilis strain 168) to amplify the pel gene. The forward primer fused the coding sequence of the AprE signal sequence to the coding sequence of the mature pel gene in frame. The forward primer also includes a BbsI restriction site, which generates an overhang sequence compatible with the BssHII site after cleavage. The reverse p...

Embodiment 2

[0425] Example 2. Characterization of pectate lyase

[0426] Under the following conditions, the pectin removal performance of Bacillus subtilis pectate lyase (SEQ ID NO: 1) was compared with that of a commercially available pectate lyase, Bioprep 3000L (Novozymes).

[0427] Pectate lyase

Total protein ＊ mg / mL

TCA protein ＊ (mg / mL)

Exemplary Bacillus subtilis of Example 1

Pectate lyase

87.8

53.4

Bioprep 3000L

(Novozymes)

44.0

35.5

[0428] " ＊ "This result is calculated based on 6.35g protein / g nitrogen. In addition, this result is an estimate of total protein.

[0429] Material: The dirt carrier used was a desizing but unbleached fabric from Testfabrics ArmyCarded Cotton Sateen (Style#428). The Bacillus subtilis pectate lyase and Novozymes Bioprep 3000L pectate lyase of Example 1 were utilized at 2 ppm total protein. Analysis of various pH 50mM BIS-TRIS propane (BTP) buffers. Use HCl to adjust the buffe...

Embodiment 3

[0441] Using acetic acid, the pectate lyase of Example 1 was formulated with propylene glycol (40%), Proxel (0.25%) (1,2-benzisothiazolin-3-one), and pH 6.25±0.1. The test was performed by incubating at 37°C for 20 minutes. Use CaCl 2 Glycine buffer with polygalacturonic acid substrate for this endpoint measurement.

[0442]

Fermented pectin

Acid lyase

Test

Volume

(μl)

pH 10

235nm

OD

Blind 210

pH 10

235nm end

OD

pH 10

APSU

Sample

APSU / ml

20040772UFC

500

0.712

0.124

0.588

0.137458362

91638.91

20040773UFC

500

0.613

0.124

0.489

0.116094893

77396.6

End of 20040772

500

0.336

0.124

0.212

0.056320337

37546.89

End of 20040773

500

0.467

0.124

0.343

0.08458917

56392.78

20040396

Whole broth

500

0.415

0.124

0...

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Abstract

Compounds, compositions, and methods for enzymatic one-step pretreatments of cellulosic and cellulosic-containing textiles are disclosed. Pretreatment comprises scouring and bleaching, and optionally desizing of the textiles in various combinations. The one-step pretreatment can also be combined with dyeing steps. Also disclosed are compositions for cleaning and stain removal, such as detergent compositions.

Description

[0001] Cross references to related applications [0002] This application claims the benefits of U.S. Provisional Application No. 60 / 846,329 filed on September 22, 2006, which is hereby incorporated by reference in its entirety. Invention field [0003] Described herein are compounds and compositions comprising pectatelyase of Bacillus subtilis, which are used in biological scouring, bleaching, dyeing, desizing, cellulose fiber treatment and cleaning compositions and combinations thereof Applications. Background of the invention [0004] Pectin polymers are a component of plant cell walls. Pectin is a heteropolysaccharide with a main chain consisting of alternating homogalacturonan (smooth regions) and rhamnogalacturonan (hairy regions), and the smooth regions are connected by 1, 4 The α-D-galacturonic acid is formed as a linear polymer. Galacturonic acid residues can be methyl esterified to varying degrees on the carboxyl group, usually in a non-random manner to completely methyl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M16/00D06L3/00D06L3/11D06L1/14D06P1/46C11D3/386C12N9/88D06L4/00D06L4/40
CPCD06M16/003D06P1/46C12N9/88D06L3/00C12Y402/02002D06L1/14D06L3/11D06L4/00D06L4/40
Inventor A·J·普洛斯B·施密特F·J·C·万加斯特尔M-Y·允
Owner DANISCO US INC
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