Trichoderma viride W2 capable of producing thermophilic ethanol-resistant beta-glucosidase and application thereof
A technology of glucosidase and Trichoderma viride, applied in the field of saccharification and fermentation of cellulosic ethanol, can solve the problem of low tolerance and the like, achieve the effects of increasing yield, solving technical bottleneck problems, and eliminating inhibition
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[0022] 1. Strain screening.
[0023] Sampling dead branches and rotten leaves, soil, fungal fruiting bodies and other materials on Wuyi Mountain. Put 100mL of distilled water in a 250mL conical flask, add dozens of glass beads, and autoclave at 121°C; add the sample into the conical flask, dissolve and shake for 1 hour. Dilute the obtained lysate by 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 1 times the concentration shaved, respectively spread to the Congo red primary screening medium, the formula of the medium is: each liter of medium contains KH 2 PO 4 0.5g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.25g, cellulose powder (microcrystalline fiber) 2.0g, Congo red 0.2g, agar 18-20g, the balance is water, pH value 7.0. Cultivate at 30°C for 2 days, pick a single colony with a large hydrolysis transparent circle on the Purified on PDA medium, and the purified strains were stored on PDA slant.
[0024] Re-screening for β-glucosidase activity of strains: Inoculate the purified str...
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