Trichoderma viride W2 capable of producing thermophilic ethanol-resistant beta-glucosidase and application thereof

A technology of glucosidase and Trichoderma viride, applied in the field of saccharification and fermentation of cellulosic ethanol, can solve the problem of low tolerance and the like, achieve the effects of increasing yield, solving technical bottleneck problems, and eliminating inhibition

Inactive Publication Date: 2011-06-01
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current cellulase is not very resistant to ethanol, which limits the application of SSF.

Method used

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  • Trichoderma viride W2 capable of producing thermophilic ethanol-resistant beta-glucosidase and application thereof
  • Trichoderma viride W2 capable of producing thermophilic ethanol-resistant beta-glucosidase and application thereof
  • Trichoderma viride W2 capable of producing thermophilic ethanol-resistant beta-glucosidase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Strain screening.

[0023] Sampling dead branches and rotten leaves, soil, fungal fruiting bodies and other materials on Wuyi Mountain. Put 100mL of distilled water in a 250mL conical flask, add dozens of glass beads, and autoclave at 121°C; add the sample into the conical flask, dissolve and shake for 1 hour. Dilute the obtained lysate by 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 1 times the concentration shaved, respectively spread to the Congo red primary screening medium, the formula of the medium is: each liter of medium contains KH 2 PO 4 0.5g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 ·7H 2 O 0.25g, cellulose powder (microcrystalline fiber) 2.0g, Congo red 0.2g, agar 18-20g, the balance is water, pH value 7.0. Cultivate at 30°C for 2 days, pick a single colony with a large hydrolysis transparent circle on the Purified on PDA medium, and the purified strains were stored on PDA slant.

[0024] Re-screening for β-glucosidase activity of strains: Inoculate the purified str...

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Abstract

The invention discloses a trichoderma viride W2 capable of producing a thermophilic ethanol-resistant beta-glucosidase and an application thereof. The trichoderma viride W2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 23, 2010, and the preservation number of the trichoderma viride W2 is CGMCC No.4098. The trichoderma viride W2 can produce a new beta-glucosidase, the enzymatic activity of the new beta-glucosidase reaches 346.7U/mL, the optimal reaction pH value is 4.8, the optimal reaction temperature is 70 DEG C, and the new beta-glucosidase is suitable for pyrohydrolysis and has an obvious glucose feedback inhibition effect. The ethanol the concentration of which is 10% has a maximal effect on promoting the enzymatic activity and improves the enzymatic activity of the beta-glucosidase by 1.6 times, and the resistant ability of the ethanol reaches 30%, so that the cellobiose inhibition can be effectively eliminated, the yield of the ethanol is improved by nearly 3 times, and the terminal product inhibition is effectively eliminated. Thus, the beta-glucosidase can be used for simultaneous saccharification and fermentation of lignocellulose raw materials, has a rare promoting effect in China, effectively increases the yield of the cellulosic ethanol, lowers the production cost, and accelerates the industrialized progress of the cellulosic ethanol.

Description

Technical field: [0001] The invention belongs to the field of saccharification and fermentation of cellulosic ethanol, and in particular relates to a kind of Trichoderma viride (Trichoderma viride) W2 producing mesophilic ethanol-resistant β-glucosidase and the produced β-glucosidase, and the β-glucosidase Application of sidase in simultaneous saccharification and fermentation. Background technique: [0002] The development of effective cellulosic ethanol conversion technology has positive significance for accelerating the industrialization process of cellulosic ethanol, increasing the diversity of liquid fuel supply, and ensuring my country's energy security and food security. Enzymatic hydrolysis is a critical step in the conversion of cellulosic ethanol. The effective hydrolysis of cellulose depends on the synergistic effect of three enzyme components: endoglucanase, exoglucanase and β-glucosidase. During the saccharification of cellulose, β-glucosidase hydrolyzes cello...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12P19/14C12R1/885
Inventor 袁振宏梁翠谊许敬亮庄新姝徐惠娟张宇亓伟
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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