Wood fibrous material coupled enzymatic saccharification method

A lignocellulosic raw material, enzymatic hydrolysis technology, applied in the direction of fermentation, can solve the problems of insufficient utilization of fiber components and high cost, and achieve the effects of reducing the usage of enzymes, increasing the yield, and improving the yield of enzymatic hydrolysis

Inactive Publication Date: 2010-07-28
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

Simply using acid or alkali to pretreat lignocellulosic raw materials not only requires an additional cumbersome neutralization process, but also the fiber components cannot be fully utilized, resulting in high cost of the pretreatment sac

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] The lignocellulosic raw material is Qingdao corn stalk, its cellulose content is 38.8%, hemicellulose content is 31.1%, lignin content is 21.2%, and the rest is ash and wax. 100g (dry) corn stalks crushed to less than 0.5cm, add 900ml of 1% sulfuric acid, reaction temperature 125°C, heating time 1h, heat preservation 20min; cool to below 90°C, filter the hydrolysis solution, and wash the filter residue with 200ml of water, Recover the hydrolyzed sugar solution and water washing solution; add 13g sodium hydroxide (analytical pure) to the filter residue, add 210g water to make the liquid-solid ratio 1:7, cook at 155°C for 20min, and heat up for 1.5h; after alkali cooking , solid-liquid separation, the liquid part is black liquor containing alkali lignin. Then mix the cellulose residue after the secondary treatment with the sugar solution of the primary hydrolysis, control the substrate concentration to 10%, adjust the pH value=4.8 by adding sodium hydroxide, and add cellu...

Embodiment 2

[0026] The lignocellulosic raw material is corn stalks from Hebei, whose cellulose content is 36.5%, hemicellulose content is 29.5%, lignin content is 19.6%, and the rest is ash and wax. Add 900ml of 2% hydrochloric acid to 100g of crushed corn stalks (dry), the reaction temperature is 128°C, the heating time is 1h, and the temperature is kept for 20min; cool to below 90°C, filter the hydrolyzed solution, and wash the filter residue with 400ml of water to recover the hydrolyzed sugar solution and washing liquid; add 6g sodium hydroxide (analytical pure) and 5.3g sodium sulfite to the filter residue, add 239g water to make the liquid-solid ratio 1:7, cook at 160°C for 20min, and heat up for 1h; after cooking, solid The liquid is separated, and the liquid part is black liquor containing alkali lignin. Then mix the cellulose residue after the secondary treatment with the sugar solution of the primary hydrolysis, control the substrate concentration to 2%, adjust the pH value=4.8 b...

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PUM

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Abstract

The invention relates to a wood fibrous material coupled enzymatic saccharification method which mainly comprises the following steps that: (1) crushed wood fibrous material is primarily pre-treated, and hemicellulose is hydrolyzed into monosaccharides or oligosaccharides solution through dilute acid or water treatment; (2) the solution in step (1) is separated from solid, residue is filtered, and hydrolyzed sugar solution and filtered residue are respectively prepared; (3) the filtered residue is cooked and delignified through a caustic soda method, a sulfate method or a sulphite method; and (4) the cellulose product which is prepared from the cooking in step (3) and is insoluble in reaction solution is filtered and mixed with the hydrolyzed sugar collected in step (2), the pH value is adjusted to be 3 to 7, cellulase, xylanase and cellobiase are added in to be hydrolyzed, and fermentable sugar is prepared. The wood fibrous material coupled enzymatic saccharification method not only reduces a neutralization step required by a one-step treatment (acid or alkali) process, but also improves the utilization rate (reducing sugar yield) of the wood fibrous material.

Description

technical field [0001] The invention belongs to the field of energy and chemical industry, and in particular relates to a lignocellulosic raw material classification pretreatment method and a coupled enzymatic hydrolysis and saccharification process. Background technique [0002] With the increasing shortage of petrochemical resources, the development and utilization of biological resources, especially agricultural and forestry wastes such as corn stalks, rice straw, wheat straw, bagasse, wood and other lignocellulosic raw materials, into liquid fuels has become a research hotspot. The biological conversion of these wastes into renewable liquid fuels such as ethanol, butanol, and biodiesel can not only solve the problem of environmental pollution, but also alleviate the increasingly tense energy crisis and reduce greenhouse gas emissions. The circular economy model is of great significance. [0003] A key step in the conversion of cellulosic biomass to bioliquid fuels is th...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 王海松牟新东刘超孙伟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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