Enzymolysis treatment method of algae

A treatment method and enzyme treatment technology, which is applied in the compound enzyme treatment of seaweed and the field of enzymatic hydrolysis of seaweed, can solve the problems of low extraction rate of sodium alginate, cumbersome operation, long enzymatic hydrolysis time, etc., and shorten the reaction time of seaweed enzymatic hydrolysis , simple operation, high total yield effect

Active Publication Date: 2016-05-18
SHANDONG HETIANWANG BIOLOGICAL TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0005] The existing technology has the following defects: (1) the extraction rate of sodium alginate is low; (2) the extraction rate of trehalan gum is low; (3) the total yield of polysaccharides is low; higher

Method used

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  • Enzymolysis treatment method of algae
  • Enzymolysis treatment method of algae
  • Enzymolysis treatment method of algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 A kind of enzymolysis treatment method of seaweed

[0031] Step 1. Material preparation

[0032] The seaweed used includes sargassum, hijiki and carrageenan, with a mass ratio of 5:2:2;

[0033] The sargassum: the water content is 15-16%; the hijiki: the water content is 12-14%; the carrageenan: the water content is 13-14%.

[0034] Step 2. Preprocessing

[0035] Wash sargassum, hijiki, and carrageenan with fresh water, dry them at 90-98°C for 40-45 minutes, grind them to 200-mesh fine powder after drying, and mix them to obtain mixed seaweed powder;

[0036] Add 3 times the mass of deionized water to the seaweed powder, and stir evenly to obtain seaweed slurry.

[0037] Step 3, enzyme treatment

[0038] Add the seaweed slurry into the reaction kettle, adjust the reaction pH, adjust the reaction temperature, add complex enzyme, the amount of enzyme added accounts for 0.7% of the mass of the seaweed homogenate; react for 60 hours, after the reaction is c...

Embodiment 2

[0042] The single factor analysis experiment of embodiment 2 reaction pH

[0043]

[0044] In the enzyme treatment step, the reaction pH of Example 2-7 is adopted, and the reaction temperature is adjusted to be 46°C. Among the compound enzymes added, papain is selected as protease, β-glucosidase is selected as cellulase, and β-glucosidase is selected as pectinase. Protopectinase; enzymatically hydrolyze seaweed according to the method of Example 1.

Embodiment 8

[0045] Embodiment 8 reaction temperature single factor analysis experiment

[0046]

[0047] In the enzyme treatment step, adopt the reaction temperature of Example 8-11, adjust the reaction pH to be 5.5, in the compound enzyme added, protease is all selected papain, cellulase is all selected β-glucosidase, pectinase is all selected original Pectinase; according to the method of embodiment 1, seaweed is enzymolyzed.

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Abstract

The invention provides an enzymolysis treatment method of algae. The method comprises the steps of material preparation, pretreatment, enzyme treatment and centrifugating. In the enzyme treatment step, the adopted enzyme is a composite enzyme which comprises proteinase, cellulase and pectinase in a mass ratio of 3:1:2. The proteinase is any one of papain, bromelin, carboxypeptidase and chymopapain. The cellulase is any one of cellobiohydrolase, endoglucanase, beta-glucosaccharase and cellobiase. The pectinase is any one of protopectinase, lyase and polygalacturonase. According to the enzymolysis treatment method of algae, the extraction rate of sodium alginate is 38.0-50.9%, and the extraction rate of alga glycine is 7.5-11.8%.

Description

technical field [0001] The invention provides an enzymatic hydrolysis method of seaweed, in particular relates to a compound enzyme treatment method of seaweed, which belongs to the technical field of biodegradation. Background technique [0002] The methods of extracting active ingredients from seaweed include chemical, physical and biological methods; the extracted active ingredients contain substances that regulate plant growth, including natural bioactive substances such as various minerals, amino acids and vitamins, which are beneficial to promote various physiological functions of plants. function. [0003] The unique seaweed polysaccharides and sodium alginate in seaweed have good biological activity, which can stimulate the production of non-specific active factors in plants and the balance of endogenous hormones, thereby promoting plant growth, increasing yield, and improving the quality of agricultural products. [0004] Among the existing methods for extracting a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/20C12P19/12C12P19/04
CPCC12P19/04C12P19/12C12P19/14C12P19/20
Inventor 王伟华陈凤霞
Owner SHANDONG HETIANWANG BIOLOGICAL TECH CO LTD
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