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Method for increasing lignocellulose saccharification yield through multi-step enzymolysis

A technology of lignocellulose and multi-step enzymatic hydrolysis, which is applied in the field of multi-step enzymatic hydrolysis to improve the yield of lignocellulose saccharification, and can solve problems affecting the utilization rate of raw materials

Inactive Publication Date: 2011-09-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increase of reducing sugar concentration, the inhibitory effect of the product is also more obvious, and the products of these enzyme synergy not only have glucose, but also certain pentoses, and pentoses are difficult to be fermented and utilized together with glucose, which affects the yield of raw materials. Utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The corn stalks pretreated by steam explosion (treatment conditions: crushed stalks below 0.5cm, exploded instantly after steam pressure of 1.9MPa and holding pressure for 7 minutes) were divided into two steps of enzymatic hydrolysis, the first enzymatic hydrolysis condition was : Substrate concentration 10%, add ZSL type xylanase 200ug / g produced by Shandong Zesheng Biotechnology Co., Ltd., adjust pH value to 4.8 with citric acid-sodium citrate buffer solution, place in 50°C water bath shaker for 48h, enzyme After the hydrolysis is completed, filter and separate to obtain the first step of hydrolyzed sugar solution, and the remaining residue is subjected to the second step of enzymatic hydrolysis. The second step enzymatic hydrolysis conditions are: substrate concentration 10%, adding Novozymes cellulase 300FPU / g, cellobiase 20IU / g, using citric acid-sodium citrate buffer solution to adjust the pH value to 4.8, and continue to React in a water-bath shaker at 50°C for ...

Embodiment 2

[0019] The cassava residue treated with 0.5% sulfuric acid at 120°C for 1 hour was enzymatically hydrolyzed in two steps. The conditions for the first step of enzymatic hydrolysis were: substrate concentration 4%, adding 100 μg / g, xylanase 720U / g, citric acid-sodium citrate buffer solution to adjust the pH value to 4.8, place it in a water bath shaker at 50°C for 48 hours, after the enzymolysis is completed, filter and separate to obtain the first step of hydrolyzing the sugar solution, and the remaining residue for the second step Two-step enzymatic hydrolysis. The second step enzymatic hydrolysis conditions are: substrate concentration 4%, adding Novozymes cellulase 350FPU / g, cellobiase 15IU / g, using citric acid-sodium citrate buffer solution to adjust the pH value to 4.8, continue to React in a water-bath shaker at 50°C for 48 hours, and filter to obtain the second-step enzymatic hydrolysis sugar solution. The sum of the total reducing sugar yields of the two-step enzymat...

Embodiment 3

[0022] The corn stalks treated with 0.4% sulfuric acid at 150°C for 30 minutes were enzymatically hydrolyzed in two or three steps. The conditions for the first enzymatic hydrolysis were: substrate concentration 8%, adding laccase produced by Dalian Inster Biotechnology Co., Ltd. 25U / g, reacted at pH 4.8 and 65°C for 8 hours. Add 650 U / g of xylanase to the fiber residue after the first step of enzymatic hydrolysis for the second step of enzymatic hydrolysis. The enzymolysis conditions are pH 4.8, 50°C, and reaction time 40h. After the second enzymatic hydrolysis is completed, the hemicellulose hydrolyzed sugar liquid is obtained by filtration and separation, and the remaining residue is subjected to the third step of enzymatic hydrolysis. The third step enzymatic hydrolysis conditions are: substrate concentration 8%, adding Novozymes cellulase 370FPU / g, cellobiase 20IU / g, using citric acid-sodium citrate buffer solution to adjust the pH value to 4.8, continue to React in a ...

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Abstract

The invention relates to a method for increasing the lignocellulose saccharification yield through multi-step enzymolysis. The method comprises the following main steps: 1) pretreating wood fiber raw material; 2) adding one or more of bio-degrading enzymes in the pretreated wood fiber stepwise or simultaneously, removing the extract component or lignin in the raw material to change the fiber structure of the raw material; 3) adding one or more of hemicellulose degrading enzymes in the lignocellulose which is removed extract or lignin through enzymolysis stepwise or simultaneously, extracting pentose in the raw material; and 4) filtering fiber residue obtained in the step 3) through the enzymolysis, and adding cellulase and / or cellobiase to hydrolyze continuously and obtain glucose hydrolysate. By adopting the method, each component can be recycled and the total reducing sugar yield can be increased.

Description

technical field [0001] The invention belongs to the field of cellulose enzymatic hydrolysis, and in particular relates to a method for increasing lignocellulose saccharification yield through multi-step enzymatic hydrolysis. Background technique [0002] Lignocellulosic raw material is an extremely abundant renewable resource in nature. If lignocellulose can be well utilized, the cost of chemical raw materials and automobile fuel will be greatly reduced. However, until now, the utilization of lignocellulosic raw materials has been insufficient. This is because lignocellulosic raw materials not only contain cellulose and hemicellulose, but also contain a certain amount of lignin and a small amount of pectin, starch, resin, terpene compounds, etc. These components are closely combined and have no effect on the external environment. It has strong resistance, hinders glycosidase from approaching fiber, and limits the hydrolysis and saccharification process of cellulose componen...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 牟新东王海松刘超孙伟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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