105results about How to "Extended fermentation cycle" patented technology

The invention relates to the range of microorganism fermentation inoculant, in particular to a microorganism composite fermentation inoculant which is used for digesting the compost of livestock manure, crop straws, mud, mushroom slag, decoction dregs, garden shearing substances, grass peat, castor bean meal, bone meal, and bean pulp meal rich in cellulose and organic matters as well as manufacturing a bio-organic fertilizer. 2 strains screened from a plurality of organic waste materials with high capability for digesting cellulose and high temperature resistance (70 DEG C) are scientifically and reasonably combined with 11 beneficial strains to prepare a microorganism composite fermentation inoculant which can not only digest organic materials, but also enhance the efficiency, quality and biological activity of the compost. The invention can be used in the high efficient and reasonable application of agricultural wastes and in the industry of waste disposal.

The invention discloses a brewing method of rice aromatic liquor. The method is described as following: after being steamed and cooked, paddy is spread, cooled and inoculated in raw starter complex wine yeast. Then semisolid is fermented under a constant temperature of 38-41 DEG C, after which the rice aromatic liquor is made by blending the distilled fermentation broth. The brewing method of the invention adopts cooked paddy for fermentation, increases the rice aromatic flavour to the rice aromatic liquor. Compared with traditional rice fermentation method, the brewing method of the invention improves wine yield rate, prolongs fermentation period, and has more flavour substances (total acid, total ester, ethyl lactate, beta-phenylethyl alcohol) in liquor finished product in different extent than the falvour substances in traditional liquor finished product.

The invention relates to the technical field of tea processing, in particular to a method for processing organic black tea. The specific process comprises the steps of spreading and airing of fresh tea leaves, water-removing, rolling, primary pile-fermenting, repeated rolling, gross fire drying, secondary pile-fermenting, steaming, pressing and aging to obtain finished products. The method can be implemented without the need of special facility equipment, and the energy consumption is effectively lowered; and the natural fermentation method is adopted, so that the fermentation period is long and the flavor and the taste of tea leaves are effectively improved.

The invention relates to a fermentation medium for fermenting and producing glutamic acid from a thermo-sensitive type strain. The components and the final concentration of the fermentation medium in the formula are as follows: 165-205g / L corn starch amylolysis, 15-30g / L maize slurry, 10-18g / L molasses, 0.5-1.5g / L glycine betaine, 5-10g / L bean pulp hydrolysate, 3-8g / L H3PO4, 1.25-1.75g / L MgSO4.7H2O, 0.064-0.084g / L MnSO4.7H2O, 0.055-0.085g / L FeSO4.7H2O, 3-6g / L KCl, 0.08-0.15g / L threonine, 0.8-1.2g / L succinic acid, and 0.1-0.3g / L defoamer, and the solvent is water, the pH value of the fermentation medium is 6.8-7.0 when the phosphoric acid is neutralized by alkali. Due to the appropriate carbon source, the nitrogen source, the carbon nitrogen ratio and the phosphorus content in the medium, the acid production rate is increased, the acid production of the thallus is promoted, the final acid production level is obviously improved by about 5.5% and the glutamic acid yield and the glucose acid conversion ratio are increased.

The invention provides an organic material decomposing agent, a humic-acid efficient bioorganic fertilizer and a manufacture method thereof. The organic material decomposing agent comprises, in percent by weight, 8%-15% of saccharomyces cerevisiae, 6%-12% of bacillus subtilis, 8%-15% of aspergillus oryzae, and 60%-72% of turf. The organic material decomposing agent is a solid powder. The bacterial strains in the provided organic material decomposing agent are specific bacterial strains capable of efficiently decomposing and utilizing lignin and cellulose, complex macromolecular compounds in straw and manure are degraded into micromolecular compounds through the bacterial strains, decomposition of straw and animal manure organic materials is promoted, and the effects of shortening compost fermentation period and improving compost product quality are realized.

The invention provides a low-cost, environment-friendly method for producing phosphorus bacteria biological fertilizer. The phosphorus bacteria biological fertilizer uses Bacillus megaterium as a production bacteria and sewage sludge as a production matrix and is produced through fermentation culture in seed tanks and fermentation tanks. The fermentation period of the whole growth process is shortened to 36-40 hours and bacillus formation rate is increased by 20%-40% compared with common methods and the live bacteria number in the fermentation liquid can reach 0.8-1 billion / ml and can reach 0.15-0.2 billion / g when the solution is absorbed with dry sterile peat. Moreover, the contents of cadmium, mercury, lead, chromium, arsenic and other heavy metals in the fermentation phosphorus bacteria fertilizer are much lower than the contents before fermentation. The pepper field experimental results show that the phosphorus bacteria biological fertilizer can increase the fertilizer utilization and output and benefit, and has positive effect to the agronomic traits such as the height of crops, width, weight, uniformity, the number of branches and the first harvest time, and meanwhile the comprehensive disease resistance is further enhanced.

The gas-phase double-dynamic alcoholization fermentation method of tobacco is characterized by that it can make the tobacco raw material placed in fermentation tank implement fermentation under the following conditions; introducing compressed air into fermentation tank and making periodic pulsed pressurization and decompression process, the pressure pressurized into the fermentation tank every time is up to 0.10-0.25 MPa, and retained in 1-50 min., then the pressure is released, and decompressed to 0.0-0.5 Mpa, and retained in 1-60 min., and the periodic pulsed circulation time from pressurization to decompression is 1-120 min., its fermentation temp. is 26-46 deg.C, humidity is 80%-95%, and the fermentation time is 20-30 day so as to implement gas-phase double-dynamic fermentation of tobacco alcoholization, and can raise colour and taste of tabacco.

The invention relates to an application of a compound leavening agent in a pork fermented sausage, discloses Leuconostoc mesenteroides with the collection number of CGMCC (China General Microbiological Culture Collection Center) 11479 and further discloses a compound leavening bacterium containing the strain. Pork is taken as a main raw material, and through inoculation of the leavening bacterium comprising staphylococcus xylosus CGMCC3474, Leuconostoc mesenteroides CGMCC11479, lactobacillus plantarum CGMCC11480 and Debaryomyces hansenii CGMCC3473, the edible safety, the trophism and the sensory quality of the pork fermented sausage are improved. Further, the pH of the fermented sausage added with the compound leavening agent is remarkably lower than that of the traditional fermented sausage, growth and propagation of harmful bacteria are inhibited effectively, and the shelf life of the pork fermented sausage can be prolonged to a certain extent.

The invention discloses a drum-type fermentation device which comprises a material inlet, a material outlet and a fermentation device body, wherein the fermentation device body comprises a first fermentation tank and a second fermentation tank which are butted mutually; at least one of the first fermentation tank and the second fermentation tank is rotatable; the butted interface part of the first fermentation tank and the second fermentation tank is arranged to be in an oppositely-rotating connection mode; the first fermentation tank and the second fermentation tank are internally provided with a first fermentation containing cavity and a second fermentation containing cavity respectively; the inner walls of the first fermentation containing cavity and the second fermentation containing cavity are both provided with spiral cutters; and the matched rotation of the first fermentation tank and the second fermentation tank enables cultivation excrement which enters the fermentation device to move from the material inlet to the material outlet or enables cultivation excrement which enters the fermentation device to be extruded from two ends to the middle part of the fermentation device. The fermentation device provided by the invention is simple in structure, convenient to operate, high in treatment efficiency, small in occupied area, short in time consumption, high in adaptability, energy-saving and environment-friendly.

The invention discloses an animal excrement harmless resource utilization intelligent treatment system and method. The system comprises a roller sieve filtering device, a fast mixing device, an anaerobic fermentation device, a marsh gas collecting device, a gas purification assembly, a gas storage device and a solid-liquid treatment assembly, wherein the roller sieve filtering device is used for removing impurities in animal excrement; the fast mixing device is used for mixing the screened animal excrement with stalks subjected to biological fermentation; the anaerobic fermentation device is used for performing anaerobic fermentation on the mixed materials; the marsh gas collecting device is used for collecting marsh gas generated in the anaerobic fermentation device; the gas purificationassembly is used for performing desulfurization, dewatering and decarburization treatment on the marsh gas collected by the marsh gas collecting device; the gas storage device is used for performing constant pressure storage on the gas purified by the gas purification assembly; the solid-liquid treatment assembly is used for separating biogas slurry and biogas residue after the fermentation, preparing the treated biogas slag into organic fertilizer, and preparing the treated biogas slurry into liquid fertilizer. The animal excrement harmless resource utilization intelligent treatment system and method have the advantages that multiple functions of excrement fermentation, marsh gas production, organic fertilizer preparation and the like are integrated; the technical process is shortened; the efficiency is greatly improved; meanwhile, a yield increase novel path is also developed for people in regions with developed breeding industry.

The invention provides high-nucleoside-yield bacillus subtilis engineering bacterium as well as a construction method and application thereof The invention also provides a method for producing nucleoside by fermentation. The method comprises the following steps of (1) enhancing a glutamate synthase gene gltA for encoding an NCBI reference sequence WP-009967365.1 on a bacillus subtilis chromosome;and / or enhancing a glutamine synthetase gene glnA for encoding an NCBI reference sequence WP-003231737.1 on the chromosome of the bacillus subtilis; and (2) applying the gene-enhanced strain obtainedin the step (1) to fermentation production of nucleoside. The bacillus subtilis engineering bacterium provided by the invention is a high-nucleoside-yield strain, can effectively accumulate nucleoside, improves the yield of nucleoside, and lays a foundation for industrial production of nucleoside.

The invention discloses a method and system for treating residual sludge by solar energy thermal radiation oxygen-enriched fermentation. The method comprises the following steps of: I, mixing solid wastes, liquid wastes, livestock manure and a dry admixture in the mass ratio of 8:1:1-7:1:2; II, performing thermal radiation oxygen-enriched fermentation at the fermentation temperature of between 50DEG C and 70 DEG C for 7 to 10 days until the moisture ratio of the mixture falls below 30 percent; and III, mixing and packaging. By adopting the method, thermal radiation oxygen-enriched fermentation is performed on residual sludge produced by urban sewage plants, so that power consumption in a fermentation process is lowered, fermentation period is shortened, floor area and running cost are reduced, and the problem of secondary pollution in the fermentation process of residual sludge is solved. The method is suitable for treating residual sludge such as sludge produced by urban sewage treatment plants, screen underflow produced by screening household garbage, livestock breeding manure, solid wastes of crop farming and gardens.

The invention relates to a fruit and vegetable waste and kitchen waste cooperative treatment process. The fruit and vegetable waste and kitchen waste cooperative treatment process comprises the stepsof fruit and vegetable waste pretreatment, kitchen waste pretreatment and cooperative treatment; at first, fruit and vegetable wastes are pretreated and kitchen wastes are also pretreated to obtain fruit and vegetable waste slurry and kitchen waste slurry, the two slurries are mixed and are treated cooperatively, and an amino acid liquid manure and humus wastes are formed by fermentation. The fruit and vegetable wastes are pretreated to form the fruit and vegetable waste slurry, the kitchen wastes are pretreated to form the kitchen waste slurry, then the fruit and vegetable waste slurry and the kitchen waste slurry are mixed at a certain ratio, thus, microorganisms are decomposed in a liquid environment, in the liquid environment, the diffusion and reproduction speeds of cultures are the highest, the reaction speed is high, and thus, the fermentation period of the fruit and vegetable wastes is shortened.

The invention discloses a production process for brewing mature vinegar from glutinous sorghum. The production process for brewing the mature vinegar from the glutinous sorghum comprises the followingsteps: 1, preparing rhizopus bran koji; and 2, carrying out a glutinous sorghum mature vinegar production process. The glutinous sorghum is used as a raw material to brew traditional mature vinegar,traditional steaming is replaced by a process of high-temperature liquidation and low-temperature saccharification, the rhizopus bran koji is added in a saccharification process, alcoholic fermentation is carried out while saccharification, and processes of solid state acetic fermentation, smoking solid-substrate fermentation, vinegar pouring, ageing and the like follow a traditional brewing process for Shanxi mature vinegar. The problem that adhesion and caking of the glutinous sorghum are easily caused after the glutinous sorghum is steamed is solved, raw materials are decomposed fully, theflavor and quality of the mature vinegar are improved, and the yield is increased.

The invention discloses a method for fermentation production of L-tryptophan with high efficiency. Through modification of a fermentation device and optimization of a fermentation medium formula and a fermentation technology, the problems of low fermentation unit and short fermentation cycle in existing production technologies are solved. The method comprises steps: a sugar supplementing system of an existing device is modified, and a single pipeline is replaced by a sugar distribution assembly through which sugar is distributed in a fermentation liquid rapidly and uniformly; the ratios of components in fermentation medium are optimized and adjusted; during the fermentation process, dissolved oxygen is controlled at a certain level through adjustment of stirring speed and air flow, the pH value is controlled through fed-batch of high-concentration ammonia water, the residual sugar content is controlled below 0.1% through fed-batch of sugar (comprising glucose and liquid sugar), and the culture is finished after 40-60h. The method prolongs the fermentation cycle, lowers the labor intensity, raises the fermentation unit greatly and lowers the production cost without addition of any extra device and manpower investment.

The invention relates to a method for the wet extraction of maize embryos, in particular to a method for producing ethanol through the wet extraction and fermentation of the maize embryos. The method comprises the following procedures of: purifying, steeping, removing stones after steeping, crushing and separating the embryos, liquefying, saccharifying, culturing yeast, fermenting, distilling, processing distilled grain, etc. The method belongs to clean production without pollution; the method adopts thick mash fermentation, thereby saving water, reducing energy consumption and reducing production cost; and colloid mill is used to solve the problem of oversize maize peel in the wet crushing of maize, thereby increasing distillation efficiency and improving output and quality.

The invention discloses a method for conducting fermentative production of citric acid by adding a saccharifying enzyme stage by stage based on pH responses. The method includes the steps that a starchy material is subjected to pulp mixing, liquefaction and inoculated fermentation to obtain end fermentation liquor; when the pH of the fermentation liquor is reduced to 2.0-3.0 in the fermentative culture process, the saccharifying enzyme is added into the fermentation liquor stage by stage in an equivalent mode every 2-8 h, wherein the total addition quantity of the saccharifying enzyme is 40-1000 U / g residual non-glucose quantity. By means of the method, the problem of saccharifying enzyme activity loss caused in the middle-late stage of citric acid fermentation can be solved, the fermentation period can be effectively shortened, the fermentation conversion rate can be increased, the residual glucose quantity of the end fermentation liquor can be reduced, wastewater discharge can be reduced, and meanwhile the activity of glucosidase secreted by glucose can be offset to reduce non-fermentable glucose. The method has significant economic benefits and environmental benefits.

The invention discloses a method for producing lipstatin by virtue of fermentation. The method comprises the following steps: preparing an inclined plane strain of streptomyces toxytricini, preparing shake flask seeds, preparing a seed solution, performing fermentation and cultivation, and adding linseed oil in a fed-batch mode after performing fermentation and cultivation for 50 hours, wherein calculated based on the volume of a fermentation canned material, the fed-batch rate of the linseed oil is 0.03-0.2L / h / t. The method disclosed by the invention can be controlled and operated more easily by only adding one substance namely the linseed oil in a fed-batch mode after performing fermentation and cultivation for 50 hours than by replenishing a mixture of linoleic acid and leucine as well as a mixture of mixed fatty acid and a leucine solution; on the premise of early fed-batch fermentation, the measures of further improving the fermentation yield, more completely utilizing a culture medium and cooling after fermenting for 130 hours can be controlled, bacterium decay can be delayed by virtue of cooling, the fermentation cycle can be prolonged by 20-30 hours, and the production titer time can be increased; and moreover, the culture medium can be used more fully and completely, the pressure of three-waste treatment can be reduced, and the method can be more environment-friendly.

The invention relates to the field of biological pharmacy, in particular to a pichia pastoris fermenting method for improving precursor expression of insulin and analogue thereof. The fermenting culture comprises the following stages of an initial growth phase, a glycerin supplementing phase and a methanol inducing phase. The method has the advantages that by controlling a formula of an initial culture medium, and the nitrogen source type and carbon source supplementing type, and continuously or semi-continuously supplementing a small amount of microorganisms or plant-source organic nitrogen sources in the middle and later period of fermenting, the fermenting cycle is extended; while the quality of the target product is guaranteed, the output is increased by not less than 30%.

The invention belongs to the technical field of fermentation and in particular discloses a process for preparing and extracting L-glutamine. The process comprises the following steps: preparing L-glutamine feed liquid, performing ultrafiltration treatment, removing mycoprotein and other macromolecular substances, performing activated carbon decolorization, strongly acidic cation exchange, vacuum concentration, isoelectric point cooling crystallization, centrifugation and the like on ultrafiltrate so as to obtain a crude product; re-dissolving the crude product, and sequentially performing weakly acidic and weakly alkaline ion exchange resin impurity removal, vacuum concentration, isoelectric point cooling crystallization, centrifugation and drying, thereby obtaining the refined L-glutamine. According to the process in the invention, the fermentation efficiency of the L-glutamine is improved, the finished product has high purity and total yield, the process is simple and convenient in production technology, the usage amount of the exchange resin is obviously decreased, and the amount of acid and alkali is greatly reduced, so that wastewater discharge is reduced, and environmental protection is facilitated.

The invention belongs to the technical field of production of amino acid and discloses a fermentation technology of L-tryptophan. The fermentation technology comprises the following steps: step one, preparing L-tryptophan fermented liquid; step two, carrying out secondary treatment on strains; step three, combining and extracting the L-tryptophan. According to the fermentation technology of the L-tryptophan, disclosed by the invention, the fermentation efficiency of the L-tryptophan is improved by treating the strains.

The invention discloses saccharomyces cerevisiae gene engineering bacteria for knocking out an FBP1 gene, and belongs to the field of microorganism genetic engineering. According to the method, aureobasidin resistance identifier AurR is used for constructing an FBP1 gene knockout assembly and successfully converting a saccharomyces cerevisiae strain. The invention further discloses a constructingmethod of the gene engineering bacteria and further discloses the application of the gene engineering bacteria in immobilization fermentation production models. The gene engineering bacteria have theadvantages that the flocculating characteristics of the saccharomyces cerevisiae in free fermentation is significantly reduced, biofilms formed in the immobilization fermentation are reduced, the adhesive is reduced, and the number of the free cell is significantly increased.

The invention belongs to the technical field of microbial fermentation, and discloses a method for producing tryptophan through fermentation of fed-batch culture liquid. In the culture process of a fermentation tank, the tryptophan is produced through a fed-batch dipotassium phosphate solution or an ammonium sulfate solution or an arginine aqueous solution in a fed-batch mode, and culture is conducted for 40 h and then completed. Under the conditions of not additionally arranging any additional equipment and not increasing any manpower input, the fermentation cycle is prolonged, the labor intensity is lowered, and the yield of the tryptophan is significantly increased.

The invention relates to a method for stable and quick production of epsilon-polylysine, and belongs to the technical field of fermentation engineering. The method for stable and quick production of the epsilon-polylysine comprises the steps that strain amylase is used for producing streptomycesdiastatochromogenes, and fermentation culture is carried out in a culture medium containing a suitable carbon source and nitrogen source. The method for stable and quick production of the epsilon-polylysine has the advantages that stable and quick fermentation production of the epsilon-polylysine is carried out by directly inoculating a spore suspension, taking a pH end point as a subcultivation index and adopting a one-step pH reduction strategy, so that the yield is increased by 80-130%, and the cycle is shortened by 28-45% compared with a conventional process; the steps of an existing production process are changed, thereby simplifying an existing fermentation production method, significantlyimproving the production intensity of the epsilon-polylysine, not only shortening the fermentation cycle and reducing the cost, but also reducing the discharge of fermentation waste liquid and exhaust gas, and reducing the environmental pollution, in addition, the process is simple and easy to enlarge, and industrialized and large-scale production is easy to achieve.

The invention provides a nutritive lily bulb ferment and a preparation method thereof. Lily bulbs, mixed fruits and sugar are used as raw materials, and mixed strains are added for fermentation, so that the nutritive lily bulb ferment is prepared, wherein the mixed fruits are a mixture of blueberries, pitayas, kiwi fruits, grapes, mulberries and black funguses; the mixed strains are a mixture of saccharomyces cerevisiae, acetobacterium balch, lactobacillus acidophilus, bifidobacterium bifidum, bacillus coagulans and aspergillus candidus; the sugar is a mixture of any two of brown sugar, levulose, xylitol, white granulated sugar and malt sugar. According to the nutritive lily bulb ferment and the preparation method thereof disclosed by the invention, the defects existing in the prior art are overcome, the lily bulbs, the kiwi fruits, the blueberries and the mixed fruits are used as the raw materials for producing the nutritive lily bulb ferment with health-care functions, and the production technology is simple.

The invention discloses capsicum-bean sauce produced by a special fermentation agent and a preparation method of the capsicum-bean sauce produced by the special fermentation agent. The method comprises the steps of adding the special fermentation agent into home-made yeast bean sauce, uniformly mixing seven-star peppers, fresh long Sichuan peppers, fresh capsicum frutescens and fried cooled rapeseed oil with raw materials such as sweety bean sauce, crystal sugar and baijiu, then, carrying out natural fermentation for 1 to 2 months in an argil jar, finally, adding garlic essential oil, pericarpium zanthoxyli essential oil, clove lilac essential oil and tea polyphenols, carrying out uniform stirring, and carrying out sealed standing for 24 to 48 hours, thereby obtaining a finished product. The capsicum-bean sauce prepared by the method is short in fermentation cycle, outstanding in fresh fragrance, low in salt content, good in color, luster and taste, full in nutritional ingredients, free of chemical additives and stable in product quality.

The invention relates to the technical field of black garlic production, in particular to a method for preparing black garlic. The method is characterized by comprising the following steps: (1) garlicto be processed is selected; (2) the garlic to be processed is mixed with a black garlic solution obtained in step (5), and then, pretreatment is performed, wherein pre-treatment temperature is 10-15DEG C, and pre-treatment pressure is 50-80MPa; (3) a product obtained in the step (2) undergoes primary fermentation under the conditions of air humidity of 60-70%RH and 40-50 DEG C, and heat-preservation fermentation lasts for another 1-2 days; (4) a product obtained in the step (3) undergoes secondary fermentation under the condition of air humidity of 60-70%RH, and heat-preservation fermentation lasts for another 1-2 days; and (5) under the condition of air humidity of 60-70%RH, a product obtained in the step (4) undergoes tertiary fermentation, and heat-preservation fermentation lasts foranother 1-2 days, and separation is performed to obtain the black garlic and the black garlic solution, wherein the temperature of the primary fermentation is lower than that of the secondary fermentation, and the temperature of the secondary fermentation is lower than that of the tertiary fermentation.

The invention discloses a closed double-layer barrel aerobic fermentation cylinder, comprising a support device, a barrel, a powder driving device, a ventilation device and a feeding and discharging system, wherein the barrel and the power driving device are respectively arranged on the support device; the barrel comprises a feeding fixed end cover, a middle rotating barrel and a discharging fixed end cover; the middle rotating barrel comprises an inner barrel and an outer barrel which are arranged along the same axial direction and are fixedly connected with each other by a connecting support plate; a material retaining seal structure is arranged between one end of the inner barrel of the middle rotating barrel and the feeding fixed end cover, and another material retaining seal structure is positioned between the other end of the inner barrel of the middle rotating barrel and the discharging fixed end cover; the feeding fixed end cover and the discharging fixed end cover are respectively connected with the middle rotating barrel by an end cover fixing device; each end cover fixing device comprises a rotation fixing structure and a rotation support structure. The closed double-layer barrel aerobic fermentation cylinder is simple in structure, high in working efficiency and degree of automation, reliable in operation and good in treatment effect, is free from odor spillover in the treatment process, and is capable of realizing harmless and resource utilization of kitchen wastes.

The invention provides an epsilon-polylysine fermentation method adopting cell immobilization and off-site and intermittent adsorption. Loofah sponge or sponge is used as a cell immobilizing carrier,and is mounted and fixed on a stirrer, a shaft and a baffle plate in a fermenting tank, then a fermentation medium is added, sterilization and cooling are performed, and a liquid seed is introduced. Cells are adsorbed to the immobilizing carrier in the fermentation process to realize cell immobilization; a sterilized organic nitrogen source solution is intermittently added in the epsilon-polylysine synthesis stage in a fed-batch manner; and off-site separation of epsilon-polylysine is carried out by ion exchange resin when the concentration epsilon-polylysine in a fermentation solution is accumulated to 3.0 g / L, and the separation process is repeated when the concentration of the fermentation solution is afresh accumulated to 3.0 g / L. Cell immobilization and off-site separation technologies are used in the epsilon-polylysine fermentation in a combination manner, and are also combined with fed-batch addition of the organic nitrogen source, so cell growth and product synthesis are effectively promoted, the synthesis period is prolonged, the fermentation yield of epsilon-polylysine is significantly improved, and the method has a practical application prospect.