3results about How to "Extended fermentation cycle" patented technology

The invention belongs to the technical field of amino acid production, and discloses a method for reducing the content of potassium ions in a culture medium, which comprises the following steps: dividing a fermentation culture medium for producing glutamic acid by microorganisms into a fermentation culture medium A and a fermentation culture medium B, and maintaining the content of potassium ions in the fermentation culture medium A to be 50% or less of that in the fermentation culture medium B. By optimizing the addition amount of potassium ions in the non-fermentation stage, the glutamic acid production rate is increased, and the fermentation period is shortened.

The invention relates to a fermentation method of erythritol, and belongs to the technical field of erythritol fermentation. The method comprises a fermentation strain shake flask culture stage, a primary seed tank expansion culture stage and a fermentation stage. The erythritol fermentation production method adopts a batch sugar supplementation mode to be put into a fermentation tank. The batch sugar supplementation fermentation is adopted, which is conducive to extending the fermentation cycle but not too long, and the time is controlled within the range of 70 to 100 hours, thereby improving the fermentation conversion rate, effectively improving the erythritol yield, and being suitable for large-scale industrial production.

The application provides a Pichia pastoris glucose oxidase production fermentation method, and belongs to the technical field of bioengineering. The fermentation method uses Pichia pastoris LY-01 gene engineering bacteria producing glucose oxidase as a starting strain, adds a fermentation medium in a fermentation tank, adds PTM1 salt after sterilization and temperature reduction, and then inoculates seed liquid for primary fermentation. When the concentration of reducing sugar in the fermentation liquid is reduced to below 0.5%, glucose solution and PTM1 salt are added for secondary fermentation. When the concentration of reducing sugar in the fermentation liquid is reduced to below 0.5% again, a mixed solution of glycerol, methanol and water and PTM1 salt are added for mixed feeding fermentation. The mixed solution of methanol and PTM1 salt is added in the fermentation liquid for induction fermentation. The enzyme activity reaches 11309 U / mL after 192 h of fermentation. In the fermentation method, the dynamic strategy of multi-stage carbon source control, especially the mixed feeding stage and the induction fermentation of methanol addition, further realizes the cell density and long-time efficient induction expression, so that high-yield glucose oxidase is obtained.