Method for preparing bio-ethanol by taking seaweed processing waste as raw material

A bioethanol and waste technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve environmental coastal ecological impacts and other issues, achieve the goal of releasing feedback inhibition, promoting opportunities, and increasing yields Effect

Inactive Publication Date: 2010-05-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only a small part of the waste is further processed into fertilizer or leftovers, and the rest of the pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Example 1: Pretreatment with sulfuric acid, enzymatic hydrolysis

[0009] (1) Enzyme preparations and strains: Cellulase was purchased from Shandong Jienuo Bioenzyme Co., Ltd., cellobiase was purchased from Sigma Company, and Saccharomyces cerevisiae was purchased from Angel Yeast, which is common in the market.

[0010] (2) Acid pretreatment of seaweed processing waste: take the kelp floating slag formed in the algin industry as raw material, after drying and pulverizing to 20-80 mesh, adding a 0.1% sulfuric acid aqueous solution to The ratio of solid to liquid is 5%, and after being treated in an autoclave at 121° C. for 1 hour, it is taken out and cooled to room temperature.

[0011] (3) Composite enzymatic hydrolysis of cellulose: Add commercially available cellulase and cellobiase to the reaction solution after the pretreatment of the cooled raw materials, and the addition amount is 0.4ml cellulase and 0.1ml cellulase per gram of seaweed processing waste. The cell...

Embodiment 2

[0014] Example 2: Pretreatment with hydrogen peroxide, enzymatic hydrolysis

[0015] (1) Enzyme preparations and strains: Cellulase was purchased from Shandong Jienuo Bioenzyme Co., Ltd., cellobiase was purchased from Sigma Company, and Saccharomyces cerevisiae was purchased from Angel Yeast, which is common in the market.

[0016] (2) Hydrogen peroxide pretreatment of seaweed processing waste: use the sargassum waste residue formed in the alginate industry as raw material, after drying and crushing to 20-80 mesh, add peroxide with a concentration of 0.2% by volume Hydrogen aqueous solution to a solid-liquid ratio of 5%, a pH value of 7.0, and react at 50°C for 6h.

[0017] (3) Composite enzymatic hydrolysis of cellulose: Add commercially available cellulase and cellobiase to the reaction solution after the pretreatment of the cooled raw materials, and the addition amount is 0.4ml cellulase and 0.1ml cellulase per gram of seaweed processing waste. For cellobiase, replenish wa...

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PUM

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Abstract

The invention relates to a method for preparing bio-ethanol by taking seaweed processing waste as raw material, which is characterized by comprising the steps: selecting the seaweed processing waste, and pretreating the raw material by sulfuric acid or hydrogen peroxide; then, adding cellulase or cellobiase for cellulose compound enzymolysis, and filtering enzymolysis liquid to remove insoluble components; and finally, supplementing inorganic salt, inoculating saccharomyces cerevisiae strain, and carrying out alcoholic fermentation under the anaerobic condition. The original structure of algae cellulose is changed by the pretreatment process, and the action probability between the cellulose and degrading enzyme can be promoted, so that the enzymolysis efficiency of the cellulose is improved; furthermore, after the cellulose is treated by the compound enzymolysis of the cellulase and the cellobiase, the feedback inhibition of the cellobiose for the cellulose can be effectively eliminated, the content of glycolysis sugar of yeast can be improved, and the yield of alcoholic fermentation is further improved.

Description

technical field [0001] The invention relates to a method for preparing bioethanol using seaweed processing waste as raw material. Background technique [0002] Bioethanol can be converted from different raw materials: sugary raw materials (sugar cane juice, molasses), starchy raw materials (corn, potatoes), and cellulosic raw materials (wood, grass, straw). Bioethanol is a kind of environmentally friendly and renewable bioenergy, which has broad application prospects in fuel substitution. At present, research on bioethanol at home and abroad is mainly focused on the selection of valuable raw materials, especially the production cost of bioethanol fuel produced from corn and sugarcane is basically comparable to that of gasoline. However, this large amount of food crops necessary for human beings is used as raw materials for ethanol production, which has caused significant fluctuations in world food prices. [0003] With the development of the seaweed processing industry, re...

Claims

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Application Information

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IPC IPC(8): C12P7/08C12R1/865
Inventor 牟海津葛蕾蕾王鹏
Owner OCEAN UNIV OF CHINA
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