Method for preparing dextran enzyme by marine bacteria Pseudoalteromonas sp.LP621 and product

A technology of Pseudomonas alternata, dextranase, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc.

Inactive Publication Date: 2009-09-02
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the dextranase production ...

Method used

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  • Method for preparing dextran enzyme by marine bacteria Pseudoalteromonas sp.LP621 and product
  • Method for preparing dextran enzyme by marine bacteria Pseudoalteromonas sp.LP621 and product
  • Method for preparing dextran enzyme by marine bacteria Pseudoalteromonas sp.LP621 and product

Examples

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Embodiment 1

[0104] Example 1. refer to Figure 1-2 . A method for producing dextranase by using Pseudoalteromonas LP621 (Pseudoalteromonassp.LP621), the steps are as follows, inoculating Pseudoalteromonas LP621 (Pseudoalteromonas sp.LP621) bacterial strains into 2216E medium, rotating speed 180r / min, 20% liquid volume, cultivated for 16 hours, to obtain seed liquid; inoculate the seed liquid with 2% inoculum in the fermentation medium, culture at 180r / min, 25°C for 24h, centrifuge at 10000r / min for 5min, and the precipitate is the bacteria Suspend the cells with buffer solution, ultrasonically crush for 5 minutes in an ice-water bath, centrifuge at 12000 r / min for 10 minutes, and take the supernatant as the crude dextranase solution.

Embodiment 2

[0105] Example 2. In the method described in Example 1, the Pseudoalteromonas sp.LP621 (Pseudoalteromonas sp.LP621) bacterial strain has the following characteristics: a Gram-negative bacillus, with capsules, without spores, and a size of 2.2-2.7 μm×0.7 —1.3 μm; the physiological and biochemical characteristics of the strain are that the strain is positive for oxidase, can ferment glucose and methyl red, cannot ferment VP, can grow without sodium chloride, can liquefy gelatin, and can produce indole; can utilize lactose, fiber Disaccharides, galactose, glycerin, alanine, glutamic acid, pyruvic acid, fructose and maltose cannot be utilized.

Embodiment 3

[0106] Example 3. refer to figure 1 . In the method described in Example 1, the colony characteristics of the described Pseudomonas alternanas LP621 (Pseudoalteromonas sp.LP621) strain on the solid medium containing dextran are: 4mm-7mm in diameter, white, moist, with smooth edges , Protruding in the middle, can produce obvious transparent circle.

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Abstract

The invention provides a method for preparing dextran enzyme by utilizing marine bacteria Pseudoalteromonas sp.LP621. The method is characterized in that: the method comprises the following steps that: strains of the Pseudoalteromonas sp.LP621 are inoculated to a 2216E culture medium under the conditions of rotating speed of 180r/min, liquid volume of 20 percent and culture time of 16h so as to obtain a seed liquid; according to 2 percent of inoculum size, the seed liquid is inoculated to a fermentation medium, and under the condition of rotating speed of 180r/min, the seed liquid is cultured at a temperature of 25 DEG C for 24h, and under the condition of rotating speed of 10,000r/min, the seed liquid is centrifuged for 5min, and the precipitations are thalli; and the thalli are suspended in a buffer solution, placed in a ice water bath for ultrasonic crushing for 5min, and centrifuged for 10min under the condition of rotating speed of 12,000r/min, and a supernatant is a coarse enzyme liquid of the dextran enzyme. The invention also relates to a dextran enzyme product obtained by the method, and the dextran enzyme product plays important role in the fields of prevention and treatment of a decayed tooth, sugar industry, production of pharmaceutical dextran and the like.

Description

technical field [0001] The invention relates to a method and a product for producing dextran by using Pseudoalteromonas sp. LP621 (Pseudoalteromonas sp. LP621) isolated from seawater in Lianyungang sea area of ​​the Yellow Sea of ​​China. Background technique [0002] Dextran (a homoglycan of D-glucopyranose) is a polysaccharide with 95% α-1,6 glycosidic bonds. Dextranase (Dextranase, α-D-1, 6-Glucan-6-D-Glucanohydrolase, EC3.2.1.11), also known as α-glucanase, is a specific cutting dextran (Dextean) Hydrolase of alpha-1,6 glycosidic bonds (Khalikova et al., 2003). Dextranase has very important application value and plays an increasingly important role in the prevention and treatment of dental caries, the sugar industry and the production of medicinal dextran (Khalikova et al., 2005). [0003] Application of dextran in the prevention and treatment of dental caries. Bacteria in the mouth ferment sucrose in food to produce water-soluble dextran. These water-soluble dextran...

Claims

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Application Information

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IPC IPC(8): C12N9/46C12R1/38
Inventor 吕明生王淑军房耀维刘姝陈丽朱广超
Owner HUAIHAI INST OF TECH
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