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A kind of method utilizing Pseudoalteromonas to prepare Vibrio anguillarum bactericide

A technology of pseudoalteromonas and vibrio eel, applied in the directions of fungicides, microorganism-based methods, botanical equipment and methods, etc., can solve the problems of probiotic harm, inflammation, secondary pollution of water bodies, etc. The effect of implementation, cheap raw materials, and easy preparation

Inactive Publication Date: 2011-12-07
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current way of using beneficial bacteria is to prepare probiotics into a bacterial suspension, mix them into bait or directly add them to the aquaculture water body. Although it can achieve a certain control effect, the large-scale use of probiotics has potential hazards, and probiotics may produce Conditional infection may also produce inflammation, and in most cases it is used together with antibiotics. Sensitive probiotics are easily killed, causing secondary pollution of water bodies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Inoculate Pseudoalteromonas sp. cells on the slant of test tube agar medium, the preparation method of 2216E medium is: peptone 5g, yeast powder 1g, FeSO 4 ·7H 2 O 0.01g, pH 7.6, aged sea water 1000mL; cultivated at 28°C for 2 days, after a large amount of bacterial lawn grew, washed the bacterial cells with normal saline, and adjusted the bacterial cell concentration to 10 7 -10 8 per mL, placed in a sterile test tube to obtain seed solution;

[0024] Use 500mL Erlenmeyer flasks to expand culture, 2216E liquid medium; fill each Erlenmeyer flask with 150m1 liquid medium, sterilize at 120°C for 20min, add 5ml seed liquid after cooling, and cultivate at 28°C, 150rpm for 2 days, Vibrio anguillarum was used as an indicator bacterium to detect the antibacterial activity of the fermentation broth. When the diameter of the inhibition zone reached the maximum, the fermentation broth was collected, the bacteria were discarded, the supernatant was ultrafiltered through a 1KDa u...

Embodiment 2

[0026] Basically the same as Example 1, the difference is: the liquid medium formula is: peptone 3g, yeast powder 0.5g, FeSO 4 ·7H 2 O 0.005g, pH 7.0, aged seawater 1000mL; inoculum size 10ml seed solution, culture temperature 25°C, culture time 1.5 days, shaker speed 180rpm.

Embodiment 3

[0028] Basically the same as in Example 1, the difference is that the formula of the liquid medium is: 4% hydrolyzate of bean cake powder, 3.5% NaCl, pH 7.6, and the remainder is 1000 mL of distilled water; the inoculum size is 5 ml of seed liquid, and the culture temperature is 28°C , the culture time was 2 days, and the shaking speed was 150 rpm.

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PUM

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Abstract

The invention relates to a preparation method of a microbial bactericide, which belongs to the field of microbial fermentation industry. The invention discloses a method for preparing Vibrio anguillaris bactericide by using Pseudoalteromonas sp., which consists of (1) slant activation and culture of strains, (2) liquid fermentation culture, (3) crude fermented liquid Mention, (4) steps such as the preparation of bacteriostatic agent are formed. The method of the invention is simple, the culture medium is cheap and easy to prepare, and the liquid culture technology is adopted, which is beneficial to enlargement and industrialized production, and has stable properties and good application value.

Description

technical field [0001] The invention belongs to the technical field of industrial microbial fermentation engineering, and in particular relates to a method for preparing a Vibrio anguillarum bactericide by using pseudoalteromonas bacteria. Background technique [0002] my country is a big aquaculture country, the output of aquatic products ranks first in the world, and aquatic products occupy an important position in my country's import and export trade. In recent years, the aquaculture industry has developed very rapidly, and the scale of intensive farming has continued to expand. However, the widespread occurrence and spread of diseases caused by high-density and large-scale aquaculture has caused serious economic losses to the aquaculture industry. In addition to nutritional factors and environmental factors, the main causes of marine fish disease are infectious pathogens such as viruses, bacteria, fungi, rickettsia and parasites. Among them, the diseases caused by bacte...

Claims

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Application Information

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IPC IPC(8): A61K35/74C12N1/20A01N63/02A01P3/00A61P31/04C12R1/01
Inventor 李俊峰李红芳张媛媛李峰夏亚穆宿烽
Owner QINGDAO UNIV OF SCI & TECH
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