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Salt-tolerant Pseudoalteromonas sp and application thereof,

A Pseudomonas alternata, salt-tolerant technology, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the serious pollution of coastal water ecosystems and other problems

Active Publication Date: 2018-09-18
HARBIN INST OF TECH AT WEIHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is the serious problem that aquaculture wastewater pollutes the ecosystem of offshore waters, and a strain of salt-tolerant Pseudomonas alternata and its application are provided.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Flocculation of Pseudomonas alternata S2-M-5 strain

[0017] The seed liquid of S2-M-5 strain was transferred into a 250 ml Erlenmeyer flask filled with 100 ml LB liquid medium according to the inoculum amount of 10%, and cultured at 30°C and 160 r / min for 72 h. Mix 10 ml bacterial solution and 1.5 ml 10% (w / v) CaCl 2 The solution was added to 1 L of 4 g / L kaolin suspension, and the pH was adjusted to 7.5. Using a ZR4-6 six-coagulation experimental mixer, stirring in sections: 400 r / min for 30 s, 150 r / min for 5 min, 70 r / min for 10 min, after standing for 5 min, measure the supernatant at 550 nm Absorbance value, calculated flocculation rate can reach 78.20%, alum flowers are obvious during coagulation, and the sedimentation speed is fast.

Embodiment 2

[0018] Embodiment 2: the COD degradation of Pseudomonas alternata S2-M-5 bacterial strain

[0019] Put the seed solution of S2-M-5 strain into a 250 ml Erlenmeyer flask filled with 100 ml simulated mariculture wastewater medium according to the inoculation amount of 1%, and the simulated wastewater formula with COD 800 mg / L is: glucose 0.1700 g, soluble starch 0.1600 g, CH 3 COONa 0.2330 g, NH 4 Cl 0.0255 g, peptone 0.1580 g, beef extract 0.0600 g, (NH 4 ) 2 SO 4 0.0284 g, KH 2 PO 4 0.0700 g, Na 2 CO 3 0.0600 g, sea crystal 33 g, deionized water 1.0 L, pH 5.5-6.0. The simulated wastewater formula of COD 400 mg / L is: glucose 0.0850 g, soluble starch 0.0800 g, CH 3 COONa0.1165 g, NH 4 Cl 0.01275 g, peptone 0.0790 g, beef extract 0.0300 g, (NH 4 ) 2 SO 4 0.0284 g, KH 2 PO 4 0.0700 g, Na 2 CO 3 0.0600 g, sea crystal 33 g, deionized water 1.0 L, pH 5.5-6.0. After culturing for 96 h at 25°C and 150 r / min, the fermentation broth was centrifuged at 5000 r / min f...

Embodiment 3

[0020] Embodiment 3: the ammonia nitrogen degradation effect of Pseudomonas alternata S2-M-5 bacterial strain

[0021] According to the inoculum amount of 2%, the seed liquid of S2-M-5 strain was put into a 250 ml Erlenmeyer flask filled with 100 ml of ammonia nitrogen medium, and cultured at 28°C and 150 r / min. Wherein the ammonia nitrogen medium formula is: (NH 4 ) 2 SO 4 0.5 g, sodium citrate 2.36 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.2 g, FeSO 4 ·7H 2 O 0.01 g, MnSO 4 4H 2 O 0.01 g, NaCl 33 g, pH 7.0-7.3. Take 2 ml of the fermentation broth in a 5 ml centrifuge tube and centrifuge at 5000 r / min for 25 min to determine the degradation rate of ammonia nitrogen is 21.27%.

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PUM

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Abstract

The invention discloses salt-tolerant Pseudoalteromonas sp and application thereof, and belongs to the technical field of coastal zone microbial technology and marine aquaculture wastewater treatment.The Pseudoalteromonas sp is Pseudoalteromonas hodoensis S2-M-5, which is isolated from sediment near a sewage outlet of Jinhai Bay Beach in Weihai City, Shandong Province, and is preserved in the General Microbiology Center of the China Microbial Culture Collection Management Committee, and the preservation number is CGMCC No. 14429. After inoculating the Pseudoalteromonas hodoensis S2-M-5 is inoculated in a suitable fermentation medium for shaking fermentation culture, the flocculation rate of a bacterial treatment suspension reaches 78.20%, the COD removal rate is 50% or more, the ammonia nitrogen removal rate is 21.27%, and the nitrite nitrogen removal rate is 40.19%. The Pseudoalteromonas hodoensis S2-M-5can be applied to the treatment of marine aquaculture wastewater due to salt tolerance and safety.

Description

technical field [0001] The invention belongs to the technical field of coastal microbial technology and mariculture wastewater treatment, and specifically relates to a strain of pseudomonas alternata which can be applied to mariculture wastewater treatment. Background technique [0002] China is currently the largest mariculture country. In 2013, the total amount of mariculture reached 80% of the world's total. In 2016, the total amount of mariculture reached 18.7563 million tons. Although my country's marine aquaculture industry has a huge output, there is still a certain gap between the aquaculture technology and model and the international advanced level. One of the main problems is the serious impact on the environment. [0003] my country's current offshore farming model needs to add a large amount of bait and medicine. Although it can save labor and improve economic benefits, this traditional farming model is not right. A large amount of residual bait and metabolites ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20
CPCC02F3/34C02F2103/20C12N1/205C12R2001/01
Inventor 王凯李斯琪程喜全王忠祥张瑛洁
Owner HARBIN INST OF TECH AT WEIHAI
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