Marine bacterium Pseudoalteromonas sp SC127 and ulva sulfuric acid bhamnosan enzyme prepared from same

Abstract

The invention aims at providing a marine bacterium Pseudoalteromonas sp SC127 and an ulva sulfuric acid bhamnosan enzyme prepared from the same. The preservation number of the strain is CCTCC NO. M 2015422, and the strain has the capability of producing the sulfuric acid bhamnosan enzyme and can be used for preparing oligomeric sulfuric acid bhamnosan. It is determined through enzyme separation and purification and SDS-PAGE that the molecular weight of the sulfuric acid bhamnosan enzyme produced from the strain is 110.6 kDa. The strain serving as a production strain of the sulfuric acid bhamnosan enzyme has the advantages of being low in nutritional requirement, high in enzyme production activity, good in stability, short in production cycle and low in cost, has certain application value in industrial production and provides a guarantee for activity research and development of oligosaccharide.

Description

technical field

[0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a strain of marine bacteria PseudoalteromonasspSC127 and Ulva rhamnosulfatase prepared therefrom. Background technique

[0002] Rhamnosulphate is a high-molecular-weight sulfated heteropolysaccharide widely present in green algae such as Ulva, Enteromorpha, and reef membranes. It is mainly composed of rhamnose and rhamnose sulfate, and also contains glucose, wood Sugar, galactose, mannose, uronic acid, etc. Studies have found that it has a variety of physiological activities, including anti-tumor, anti-virus, anti-coagulation, hypoglycemic and blood lipid, immune regulation, antioxidant activity and other effects. It is a class of compounds with important biological activities and very medicinal value. However, due to the huge molecular weight of rhamnosan sulfate, the molecular weight distribution ranges from tens of thousands to millions, which seriou...

Examples

Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with the examples.

[0019] 1. Screening of strain Pseudoalteromonassp.SC127

[0020] Microbial enrichment, primary screening, and secondary screening were carried out on seaweed mud samples to obtain enzyme-producing bacteria. Carry out liquid culture to the enzyme-producing bacteria, extract the extracellular product from the fermented bacteria liquid, and obtain the rhamnosulphacanase.

[0021] The specific technical scheme is as follows:

[0022] 1. Enrichment culture: Take an appropriate amount of green algae samples under sterile conditions, add sterile seawater to mash, take 2.5mL of samples and insert them into 50mL enrichment medium (rhamnosulphate 0.3%, peptone 0.5%, sodium nitrate 0.4 %, 0.05% anhydrous magnesium sulfate, 0.15% dipotassium hydrogen phosphate, prepared from old sea water, with natural pH), and cultivated at 25°C and 150rpm on a constant temperature shaker for 7 days. Aft...

technical field

[0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a strain of marine bacteria PseudoalteromonasspSC127 and Ulva rhamnosulfatase prepared therefrom. Background technique

[0002] Rhamnosulphate is a high-molecular-weight sulfated heteropolysaccharide widely present in green algae such as Ulva, Enteromorpha, and reef membranes. It is mainly composed of rhamnose and rhamnose sulfate, and also contains glucose, wood Sugar, galactose, mannose, uronic acid, etc. Studies have found that it has a variety of physiological activities, including anti-tumor, anti-virus, anti-coagulation, hypoglycemic and blood lipid, immune regulation, antioxidant activity and other effects. It is a class of compounds with important biological activities and very medicinal value. However, due to the huge molecular weight of rhamnosan sulfate, the molecular weight distribution ranges from tens of thousands to millions, which seriou...

Examples

Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with the examples.

[0019] 1. Screening of strain Pseudoalteromonassp.SC127

[0020] Microbial enrichment, primary screening, and secondary screening were carried out on seaweed mud samples to obtain enzyme-producing bacteria. Carry out liquid culture to the enzyme-producing bacteria, extract the extracellular product from the fermented bacteria liquid, and obtain the rhamnosulphacanase.

[0021] The specific technical scheme is as follows:

[0022] 1. Enrichment culture: Take an appropriate amount of green algae samples under sterile conditions, add sterile seawater to mash, take 2.5mL of samples and insert them into 50mL enrichment medium (rhamnosulphate 0.3%, peptone 0.5%, sodium nitrate 0.4 %, 0.05% anhydrous magnesium sulfate, 0.15% dipotassium hydrogen phosphate, prepared from old sea water, with natural pH), and cultivated at 25°C and 150rpm on a constant temperature shaker for 7 days. Aft...

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