Application of pseudoalteromonas sp. to preparation of nano-material

A technology of pseudoalteromonas and nanomaterials, which is applied in the application field of pseudoalteromonas in the preparation of nanomaterials, can solve the problems of chemical sludge consumption of chemical agents, high operating costs, complicated processes, etc. Cadmium performance, saving raw materials, simple operation effect

Active Publication Date: 2016-08-03
SHANDONG UNIV
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  • Application Information

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Problems solved by technology

At present, the biological treatment of cadmium and phosphorus in wastewater mainly relies on adsorption and anaerobic phosphorus removal, the process is complicated, and it is necessary to control aeration conditions and sludge age.
In terms of high-salt seawater wastewater treatment, the method of acclimating salt-tolerant activated sludge is mainly used, but it takes a long time and high operating costs. The high-efficiency sedimentation biological filtration process based on chemical precipitation consumes a large amount of chemical agents and produces chemical sludge.

Method used

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  • Application of pseudoalteromonas sp. to preparation of nano-material
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  • Application of pseudoalteromonas sp. to preparation of nano-material

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: Pseudoalteromonas sp. (Pseudoalteromonassp.) DSBS prepares nanomaterials in low-salt wastewater

[0037] (1) Pseudoalteromonas sp. (Pseudoalteromonassp.) DSBS was inoculated in liquid LB medium at 0.9% (v / v), and cultured in a constant temperature shaker at 25° C. and 200 rpm for 20 hours to obtain enriched bacteria.

[0038] (2) The enriched bacterial suspension was inoculated in low-salt wastewater at 10% (v / v), and cultured in a constant temperature shaker at 25°C and 200rpm for 48 hours to obtain a bacterial suspension containing cadmium-phosphorus-sulfur nanoparticles. liquid.

[0039](3) The bacterial suspension containing cadmium-phosphorus-sulfur nanoparticles was centrifuged and washed with deionized water. The centrifugation speed is 3000rpm, and low-speed centrifugation is used to prevent the elution of particles on the surface of the bacteria, and the nanomaterials are prepared.

[0040] Described liquid LB culture medium component is as foll...

Embodiment 2

[0047] Embodiment 2: Pseudoalteromonas sp. (Pseudoalteromonassp.) DSBS prepares nanomaterials in high-salt wastewater

[0048] (1) Pseudoalteromonas sp. (Pseudoalteromonassp.) DSBS was inoculated in liquid LB medium at 0.9% (v / v), and cultured in a constant temperature shaker at 25° C. and 200 rpm for 20 hours to obtain enriched bacteria.

[0049] (2) Centrifuge the enriched bacteria at 3000 rpm for 10 min, discard the supernatant, and resuspend to the original volume with high-salt wastewater to obtain the bacteria washed once.

[0050] (3) Inoculate the washed bacterial suspension in three groups of high-salt wastewater at 0.2‰ (v / v), and cultivate it in a constant temperature shaker at 25°C and 200rpm for 48h to obtain cadmium-phosphorus-sulfur nanoparticles. bacterial suspension.

[0051] (4) The bacteria suspension containing cadmium-phosphorus-sulfur nanoparticles was washed with deionized water at a centrifugation speed of 3000 rpm, and low-speed centrifugation was use...

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Abstract

The invention discloses an application of pseudoalteromonas sp. DSBS to preparation of a nano-material. The pseudoalteromonas sp. DSBS is collected in China Center for Type Culture Collection (CCTCC) on December 12, 2013, with an address of Luojiashan, Wuchang, Wuhan and a collection number of CCTCC M2013652. The invention furthermore discloses the cadmium-phosphorus-sulfur nano-material, which is biologically synthesized from the pseudoalteromonas sp. DSBS in a water body containing cadmium and phosphorus. According to a strain, nanoparticles can be synthesized in cadmium-phosphorus unsaturated systems with different salinities, and cadmium and phosphorus in the water body can be removed. The substrate utilization rate is high and outlet water is free of cadmium and phosphorus pollution.

Description

technical field [0001] The invention relates to the application of pseudoalteromonas in the preparation of nanometer materials. Background technique [0002] Nanomaterials have unique physical characteristics and chemical properties, and can be used in the fields of sensors, catalysis, semiconductors, drug delivery, pollutant degradation, and environmental remediation, and have attracted extensive attention from academic and engineering circles in recent years. [0003] At present, the synthesis of metal nanomaterials mainly includes physical, chemical and biological methods. Physical methods include evaporation and condensation, physical pulverization, and mechanical alloying, but their output rate is low, energy consumption is high, and cost is high. Chemical methods include chemical precipitation, hydrothermal synthesis, template method, etc. However, it is not easy to control the size, shape and structure of the synthesized nanoparticles; it needs harsh conditions such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12N1/20C02F3/34B82Y40/00B82Y5/00B01J27/14C12R1/01C02F101/20C02F101/14C02F101/10
CPCB01J27/14B01J35/004B82Y5/00B82Y40/00C02F3/34C02F2101/006C02F2101/101C02F2101/105C02F2101/14C02F2101/20C12N1/20C12P3/00Y02P20/133Y02P20/59
Inventor 周维芝王美张海欧刘东生赵海霞
Owner SHANDONG UNIV
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