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Bacterial exopolysaccharide-based drinking water heavy metal adsorbent

A technology of bacterial exopolysaccharides and exopolysaccharides, which is applied in the field of water purification to achieve stable performance, low cost and simple operation

Active Publication Date: 2017-12-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General Microbiology Center (CGMCC), China Committee for Culture Collection of Microorganisms, is located at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A marine bacterium used for the adsorption of heavy metals was isolated and screened from the spitting mud of Philippine clams cultured in a marine farm in Zhujiajian, Zhoushan, Zhejiang, China, and was identified as Pseudoalteromonas ( Pseudoalteromonassp.), was deposited on August 29, 2016 in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures, with the preservation number CGMCCNo. 12913. The General Microorganism Center (CGMCC) of China Committee for Culture Collection of Microorganisms is located at No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0020] The preparation method of the drinking water heavy metal adsorbent based on bacterial exopolysaccharide comprises the following steps:

[0021] 1) Seed liquid culture: inoculate the marine bacteria with the preservation number CGMCC No. 12913 on the slant medium, cultivate at a constant temperature of 26°C for 36 hours, add sterile water equal t...

Embodiment 2

[0028] The most preferred preparation method of the drinking water heavy metal adsorbent based on bacterial exopolysaccharide comprises the following steps:

[0029] 1) Seed liquid culture: the marine bacteria with the preservation number of CGMCC No. 12913 Pseudoalteromonas The sp.GHS18 strain was inoculated on the slant medium and cultured at a constant temperature of 25°C for 36h. Add an equal volume of sterile water and shake to obtain a seed solution. The composition and concentration of the slant medium are glucose 22g / L, urea 0.47g / L, ammonium sulfate 0.2g / L, yeast extract 0.5g / L, dipotassium hydrogen phosphate 2.7g / L, potassium dihydrogen phosphate 1.1g / L , agar 13g / L and the remainder aged seawater or artificial seawater; adjust the pH to 7.3, and autoclave at 121°C for 25min. Among them, the artificial seawater formula is: sodium chloride 25.0 g / L, magnesium chloride 1.9 g / L, magnesium sulfate 3.1 g / L, calcium chloride 1.3 g / L, sodium bicarbonate 0.2 g / L, potassium...

Embodiment 3

[0034] Embodiment 3: the detection of adsorbent to manganese ion adsorption effect

[0035] 1) Prepare a manganese ion solution with a concentration of 0.5mg / L;

[0036] 2) Weigh 10g of wet heavy metal adsorbent into a 150mL Erlenmeyer flask;

[0037] 3) Heat the solution to boiling and then cool slightly. Measure 50mL of manganese ion solution into conical flasks filled with adsorbent, and shake for 10 minutes to fully contact the adsorbent and manganese ion solution. Another 50mL solution was taken as the water sample before treatment;

[0038] 4) Centrifuge the water sample after adsorption (10000×g, 20min) to remove the residual adsorbent in the water. After treatment, take two 10mL water samples respectively in two 25mL colorimetric tubes, set the volume to 25mL, and mark 1 and 2. Water sample 2 is a parallel control;

[0039] 5) Take 10mL of the water sample before treatment in a 25mL colorimetric tube, set the volume to 25mL, and mark it;

[0040] 6) Measure the m...

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PUM

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Abstract

The invention discloses a bacterial exopolysaccharide-based drinking water heavy metal adsorbent, which is prepared from exopolysaccharides produced by a marine bacterium Pseudoalteromonas sp. GHS18 strain with the preservation number of CGMCC No. 12913, agar and starch, wherein the mass ratio of the agar to the starch is (1 to 2) : (2 to 1). The beneficial effects are as follows: the prepared adsorbent can adsorb various heavy metal such as cadmium, lead and copper in drinking water, has the advantages of high adsorption efficiency and stable performance, and overcomes the defects of potential toxicity of inorganic polymeric adsorbents and synthetic organic polymeric adsorbents to organisms, thus ultimately achieving non-polluting emission. When being used, the adsorbent just needs to be put into the drinking water, the use is convenient, and drinking water quality is improved.

Description

technical field [0001] The invention relates to the technical field of water purification, in particular to a drinking water heavy metal adsorbent based on bacterial exopolysaccharide. Background technique [0002] Heavy metals generally refer to metals with a specific gravity greater than 4.0 and an atomic number above 24 in the periodic table. The heavy metals that pollute the water environment mainly include arsenic, mercury, cadmium, lead, chromium, copper, zinc, nickel, manganese, silver, etc. Among them, arsenic, mercury, cadmium, and lead are the most toxic and difficult to handle. The most common heavy metal pollutants. [0003] Due to the heavy metal pollution of the water source, the heavy metal content of the tap water prepared by the waterworks reaches a critical value or exceeds the standard in some cases; due to the use of old water supply pipe networks, the heavy metals such as lead in the originally qualified tap water exceed the standard during the transpor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/28C02F101/20C12R1/01
CPCC02F1/286C02F2101/20C12N1/20C12P19/04C12N1/205C12R2001/01
Inventor 穆军穆思源崔霞王玉霞
Owner ZHEJIANG OCEAN UNIV
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