Culture medium for Pseudoalteromonas sp.DHQ25 and preparation method thereof

A technology of DHQ25 and Pseudomonas, which is applied to the culture medium and preparation field of Pseudomonas Alternaria DHQ25

Inactive Publication Date: 2011-08-03
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic research on algicides and their active substances is still in its infancy. The efficient and specific screening of algicides, biodegradable algicides, especially active proteins, has provided a basis for the development and utilization of new algicides. a new way of thinking

Method used

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  • Culture medium for Pseudoalteromonas sp.DHQ25 and preparation method thereof
  • Culture medium for Pseudoalteromonas sp.DHQ25 and preparation method thereof
  • Culture medium for Pseudoalteromonas sp.DHQ25 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 PB design

[0043] This PB design (X 1 ) peptone, (X 2 ) yeast powder, (X 3 ) Glucose, (X 4 )NaCl, (X 5 )MgSO 4 .7H 2 O, (X 6 )CaCl 2 .2H 2 O, (X 7 )KCl, (X 8 )K 2 HPO 4 , (X 9 )KH 2 PO 4 , (X 10 )Fe 2 (SO 4 ) 3 The selected factors and their initial levels are shown in Table 1. Among them, -1 represents a low level, +1 represents a high level, and the response value Y is the bacterial concentration (OD 600 ). The PB design table and experimental results are shown in Table 2, and the analysis of variance using software for this result is shown in Table 3, (X 1 ) peptone and (X 2 ) Yeast powder is the main influencing factor, they are all significant at the 0.05 level, and the order of significance is: peptone>yeast powder. The remaining factors were not significant, so the concentration was controlled at the central point (see Table 1) as a constant in subsequent experiments, and the effect of peptone and yeast powder on the response...

Embodiment 2

[0059] Embodiment 2 The fastest ascent experimental design

[0060]From the fitted first-order model, it can be known that the coefficients of peptone and yeast powder are both positive, indicating that the increase in their amount will be conducive to the increase of bacterial cell concentration. Therefore, to move along the fastest ascending path, it is necessary to follow the path where both peptone and yeast powder increase. Direction for climbing experiments. The experimental design and results are shown in Table 6. The design stipulates that the basic step length λ1 of peptone normative variable = 1, then the basic step length λ2 of the normative variable of yeast powder and their conversion with the actual step lengths Δξ1 and Δξ2 of peptone and yeast powder are as follows:

[0061] λ2=(0.1045 / 0.166)×λ1=0.63 Δξ1=λ1×1=1 Δξ2=λ2×0.5=0.315

[0062] It can be seen from Table 6 that when the concentration of peptone and yeast powder increased to 13g / L and 4.15g / L at point 10...

Embodiment 3

[0065] Example 3 Response surface design

[0066] According to the results of the fastest ascent experiment, take the origin + 10ξ as the center point, double the step length in the fastest ascent experiment as the central combination design step size, select the number of center experiments as 5, and the asterisk arm length as 1.414, except for the yeast powder and peptone The amount changes, and other factors are fixed at the center point for experiments. The central combination design and results are shown in Table 7.

[0067] Table 7 Central combination design

[0068]

[0069] The first 9 experiments in Table 7 are actually 2 2 In the experiment, the analysis of variance (Table 8) carried out on this shows that the first-order item and the interaction are all non-significant, indicating that the first-order model is not suitable, the pure quadratic item is significant, and the test results are close to the optimal point. The set of experiments constituted a 5-level ...

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Abstract

The invention discloses a culture medium for Pseudoalteromonas sp.DHQ25 and a preparation method thereof, relates to a culture medium, and provides a culture medium suitable for culturing the Pseudoalteromonas sp.DHQ25 and capable of obviously improving cell density of the Pseudoalteromonas sp.DHQ25, and a preparation method thereof. The culture medium consists of 13 to 13.5g / L of peptone, 4 to 4.5g / L of yeast powder, 0.05 to 0.15g / L of glucose, 20 to 30g / L of NaCl, 0.1 to 0.3g / L of MgSO4.7H2O, 0.4 to 0.6g / L of CaCl2.2H2O, 0.03 to 0.09g / L of KCl, 0.04 to 0.06g / L of K2HPO4, 0.04 to 0.06g / L of KH2PO4, and 0.0015 to 0.0025g / L of Fe2(SO4)3, and distilled water is used for fixing the volume of 1L. The preparation method comprises the following steps of: designing an experiment by using power builder (PB); performing a steepest ascent experiment; and performing a central composite design.

Description

technical field [0001] The present invention relates to a culture medium, in particular to a Pseudomonas alternate bacteria DHQ25 which adopts the combination of PB design method and response surface method to determine its optimal culture medium composition to obtain higher cell density and algicidal active substances Culture medium and its preparation method. Background technique [0002] Red tide is a harmful ecological phenomenon in which some phytoplankton, protozoa or bacteria in seawater explode to proliferate or highly gather under certain environmental conditions, causing the water body to change color. The occurrence of red tides has a huge negative impact on the ecological balance of the ocean, the health of all human beings, and the economic and social benefits of the marine industry. At present, red tides have become a worldwide public hazard, and more than 30 countries and regions, such as the United States, Japan, China, Canada, the Philippines, India, Indone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/38
Inventor 郑天凌吕静琳王宾香郑伟田蕴
Owner XIAMEN UNIV
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