Sulfur oxidizing bacteria and application thereof

A technology of sulfur oxidizing bacteria and strains, applied in the field of environmental treatment, can solve problems such as corrosion, instability, secondary pollution of waste liquid, etc., and achieve the effects of solving pollution, promoting secondary utilization, and efficiently exerting desulfurization functions

Active Publication Date: 2021-07-23
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its limitations are: the chemical absorption method needs to add a series of chemical reagents, which will cause corrosion to sewage treatment structures such as deodorization devices and various pipelines; when using strong acid or strong alkali, it is necessary to consider operational safety factors, and the waste liquid produced after absorption prone to secondary pollution
Therefore, the oxidation of sulfur oxidizing...

Method used

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  • Sulfur oxidizing bacteria and application thereof
  • Sulfur oxidizing bacteria and application thereof
  • Sulfur oxidizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Pseudoalteromonas ( Pseudoalteromonas sp. ) Isolation and identification of LZB5-2

[0034] 1. Isolation of strains

[0035] It was isolated from seawater sediment collected from Laizhou Bay, and then isolated by streaking on 2216E medium to obtain strain LZB5-2.

[0036] 2. Identification of strains

[0037] 2.1. Morphological identification

[0038] The bacterial strain LZB5-2, which is in the logarithmic growth phase and has a stable colony size, was isolated and purified above for identification. It is white and translucent with smooth edges, and the bacterial strain is a Gram-negative bacterium.

[0039] 2.2 Homology analysis of 16S rDNA sequence

[0040] The 16S rDNA fragment of the obtained strain LZB5-2 was amplified by colony PCR method. The 16S rRNA gene fragment was amplified and cloned and sequenced. The results showed that the 16S rDNA of the strain LZB5-2 had the nucleotide sequence of sequence 1 in the sequence table. The 16SrDNA of bacterial strain...

Embodiment 2

[0045] 1. Culture method of strain

[0046] The 2216E medium was used to cultivate (purchased finished medium) the LZB5-2 strain, and the culture condition was 30°C.

[0047] 2. Analysis of intermediate metabolites in the degradation process of sodium sulfide

[0048] 1) Transfer the above-mentioned preserved strains to a plate with sodium thiosulfate as the substrate;

[0049] 2) Inoculate the purified preserved strain with an inoculation amount of 5wt% in the inorganic salt basic culture medium with thiosulfate as the substrate to verify whether it has degradation ability. If turbidity occurs, the bacterial solution can be used for The next step is to:

[0050] Into a conical flask containing 150 mL of inorganic salt basic culture solution, add 15 mL of bacterial solution (when the bacterial solution OD600nm=0.22, the concentration of the bacterial solution is about 0.44ⅹ10^9 cfu / ml), and place at 30 °C, 160 r / min shaker cultivation, during the cultivation process, sample...

Embodiment 3

[0067] Embodiment 3 organic fertilizer preparation:

[0068] 1) Raw materials: Mix raw materials according to the ingredients recorded in Table 1, and control the initial moisture content (60±1)% after mixing; then use aerobic composting to ferment, specifically select the film-covered fermentation process, and cool the fermented product to room temperature, and it is packaged after being sterilized and sterilized to obtain a finished product.

[0069] Table 1 List of raw materials

[0070]

[0071] The sludge in Table 1 is the sludge from Zhoushan urban sewage treatment plant, specifically Baiquan Town, Dinghai District, Zhoushan City, Zhejiang Province (longitude 122.19, latitude 30.10°), and the temperature changes in this place are as follows Figure 6 As shown, in Zhoushan City Daobei Sewage Treatment Plant, mushroom residues can be collected according to conventional records, and then crushed to 40 meshes for use. Small wooden block is the small wooden block of 1-2c...

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Abstract

The invention relates to the field of environmental treatment, and in particular relates to sulfur oxidizing bacteria and application thereof. The sulfur oxidizing bacteria are Pseudoalteromonas sp. LZB5-2, and have been preserved in the China General Microbiological Culture Collection Center on January 29, 2021; the preservation address is Beijing, China; the preservation number is CGMCC NO.21762; and the classification name is Pseudoalteromonas sp. According to the invention, sludge, sewage waste and the like which cannot be directly utilized are comprehensively utilized by utilizing microorganisms; and the invention can also be applied to aquatic product culture.

Description

technical field [0001] The invention relates to the field of environmental treatment, in particular to a sulfur-oxidizing bacterium and its application. Background technique [0002] With my country's increasing emphasis on environmental protection and huge investment in sewage treatment, urban sewage has been effectively collected and treated, and a large amount of municipal sludge has been produced in the process. Sludge contains pathogens, parasites, heavy metals, refractory organic matter, carcinogenic organic matter and other toxic substances, which can cause serious environmental pollution. At the same time, urban sludge is rich in N, P, K, Ca and other elements. The sludge after harmless treatment can improve the physical and chemical properties of the soil, increase the yield of crops, and has certain agricultural application value. Sludge treatment and disposal include sludge treatment and sludge disposal, with the purpose of harmless treatment of sludge and avoidi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05F17/20B01D53/84C12R1/01
CPCB01D53/84B01D2251/95C05F5/00C05G3/00C05F17/20C05F5/002C05F7/00C05F11/00C05F11/08Y02W30/40Y02A50/20
Inventor 冯大伟宋宛霖李丁军姚振领张建柏
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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