Melanin-producing marine bacterium and fermentation method for producing natural melanin

A natural melanin, production method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of high requirements for melanin conditions, poor stability, long growth cycle, etc.

Inactive Publication Date: 2011-11-02
SHANDONG UNIV AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, the isolation and purification of autobiological species requires high conditions f...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment example 1

[0018] 1. Activation and purification of the strain: DH166 can grow on the 2216E medium with the initial pH of 7.2 and Chen Haihai as the medium water. The colony is black with a smooth surface and a diameter of 2-3 mm. Visible melanin production. Colonies with obvious black surroundings were picked for further cultivation.

[0019] 2. Liquid culture of bacterial strains: control beef extract 0.5%, peptone 0.5%, sea water is used as the culture medium to prepare liquid culture medium, control the medium salinity to be 3%, divide into 1000ml triangular flasks with a capacity, and adjust the pH to 7.2 , the culture condition is 28°C controlled by a shaker, and the growth condition is better.

[0020] 3. High-density cultivation of fermentation equipment: high-density cultivation of fermentation tanks; with glucose 0.15%, peptone 0.56%, L-Ty, 2.5%, CaCl 2 0.01%, natural seawater is used as medium water, and the initial pH is 7.2. Under the culture conditions, it can grow rapidl...

specific Embodiment example 2

[0023] 1. Activation and purification of the strain: DH166 can grow on the 2216E medium with the initial pH of 7.6 and Chenhai sea water as the culture medium. The colony is black with a smooth surface and a diameter of 2-3 mm. Visible melanin production. Colonies with obvious black surroundings were picked for further cultivation.

[0024] 2. Liquid culture of bacterial strains: control beef extract 0.5%, peptone 0.5%, sea water as medium water to prepare liquid medium, control medium salinity to 3%, divide into 1000ml Erlenmeyer flasks, adjust pH It is 7.6, and the culture condition is 28 ℃ controlled by shaking table, and the growth condition is better.

[0025] 3. High-density cultivation of fermentation equipment: high-density cultivation of fermentation tanks; with glucose 0.15%, peptone 0.56%, L-Ty, 2.5%, CaCl 2 0.01%, natural seawater is used as medium water, and the initial pH is 7.6. Under the culture conditions, it can grow rapidly and melanin output can be seen. ...

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PUM

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Abstract

The invention belongs to the technical field of microbiological research and relates to a melanin-producing marine bacterium and a fermentation method for producing natural melanin. The melanin is extracted from the culture product of a marine bacterium. The bacterium needs to be cultured in a medium containing L-tyrosine (L-Tyr) and supplemented with glucose, peptone, beef extract or beef powder and aged seawater, under such a condition that the initial pH value ranges from 6.1 to 7.8. The culture product is subjected to high-speed centrifugation to remove the bacterium, the pH value of the supernatant is adjusted to the range from 2 to 4, the supernatant is incubated at room temperature and then centrifuged to remove foreign matters, and the resulting product is dried until the weight is constant and then eluted with organic solvents to produce the purified melanin (i.e. natural melanin). The bacterium is a strain of Pseudoalteromonas sp. and has a preservation serial number of CGMCC No.1782. The natural melanin has radioprotective and antioxidant effects. The production method has low cost and high yield and can be widely applied.

Description

Technical field: [0001] The invention belongs to the technical field of microorganism research. The invention relates to a marine bacterium producing melanin and a method for fermenting and producing natural melanin. The natural melanin has anti-radiation and anti-oxidation effects, the production method is low-cost, the output is high, and the application is wide. Background technique [0002] Pigments are widely used in food, printing and dyeing, cosmetics, medicine, plastics, pigments, etc. in human production and life. Since the toxic effects of chemically synthesized pigments on the human body are often reported, compared with synthetic pigments, natural pigments are environmentally friendly, non-polluting, and harmless to the human body. Therefore, the research and application of natural pigments have received extensive attention and are extremely socially acceptable. At the same time, the development and utilization of marine biological resources has been paid more...

Claims

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Application Information

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IPC IPC(8): C12P1/04C12N1/20C12R1/01
Inventor 杜宗军邢翔陈冠军
Owner SHANDONG UNIV AT WEIHAI
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