Culture medium for producing carrageenase by using pseudoalteromonas fermentation and fermentation method

A technology of alternomonas and fermentation medium, which is applied in the field of medium for producing carrageenase by fermenting Pseudomonas carrageenan, and can solve the problem of low activity, limited application, poor stability of carrageenase, etc. question

Inactive Publication Date: 2015-05-13
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the main source of carrageenase is marine microorganisms, the particularity of the marine environ

Method used

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  • Culture medium for producing carrageenase by using pseudoalteromonas fermentation and fermentation method
  • Culture medium for producing carrageenase by using pseudoalteromonas fermentation and fermentation method
  • Culture medium for producing carrageenase by using pseudoalteromonas fermentation and fermentation method

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1: Screening of Carrageenan Pseudoalteromonas ASY5

[0030] In the present invention, a carrageenan-eating Pseudoalteromonas ASY5 ( Pseudoalteromonas sp.ASY5) (preservation number: CICC23819), a solid medium was prepared with carrageenan as the sole carbon source, cultured at 20°C for 48 hours after inoculation, hydrolysis circles or pits formed after the degradation of carrageenan appeared on the plate, such as figure 1 shown.

Embodiment 2

[0031] Embodiment 2: Carrageenase produced by shake flask fermentation of Carrageenan Pseudoalteromonas ASY5

[0032] (1) After thawing the glycerol tube strains stored at -20°C, they were placed on a plate containing strain activation medium, and cultured in a 20°C incubator for 24 hours to obtain activated Pseudomonas carrageenans ASY5 strains kind. The strain activation medium is: beef extract 10 g / L, tryptone 10 g / L, agar 20 g / L, NaCl 21.1 g / L, KCl 0.58 g / L, CaCl2 0.811 g / L, MgCl 2 ·6H 2 O 3.6 g / L, NaHCO 3 0.083 g / L, MgSO 4 ·7H 2 O 2.625 g / L, adjust the pH to 7.3. Sterilize at 121°C for 20 min;

[0033] (2) The cultured strains were picked from the plate and placed in a 250 mL shake flask containing 40 mL seed medium, and fermented at 20°C and 180 r / min for 24 h to obtain the shake flask seed liquid. The seed medium is: peptone 5 g / L, yeast extract 1 g / L, NaCl 30 g / L, MgSO 4 ·7H 2 O 5 g / L, KCl 1 g / L, CaCl 2 0.2 g / L, K 2 HPO 4 0.1 g / L, FeSO 4 ·7H 2 O 0.02 g...

Embodiment 3

[0036] Embodiment 3: Carrageenase is produced by fermentation on tank of carrageenan-eating Pseudoalteromonas ASY5

[0037] (1) After thawing the glycerol tube strains stored at -20°C, they were placed on a plate containing strain activation medium, and cultured in a 20°C incubator for 24 hours to obtain activated Pseudomonas carrageenans ASY5 strains kind. The strain activation medium is: beef extract 10 g / L, tryptone 10 g / L, agar 20 g / L, NaCl 21.1 g / L, KCl 0.58 g / L, CaCl 2 0.811 g / L, MgCl 2 ·6H 2 O 3.6 g / L, NaHCO 3 0.083 g / L, MgSO 4 ·7H 2 O 2.625 g / L, adjust the pH to 7.3. Sterilize at 121°C for 20 min;

[0038] (2) The cultured strains were picked from the plate and placed in a 250 mL shake flask containing 40 mL seed medium, and fermented at 20°C and 180 r / min for 24 h to obtain the shake flask seed liquid. The seed medium is: beef extract 10 g / L, tryptone 10 g / L, NaCl 21.1 g / L, KCl 0.58 g / L, CaCl 2 0.811 g / L, MgCl 2 ·6H 2 O 3.6 g / L, NaHCO 3 0.083 g / L, MgSO ...

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Abstract

The invention discloses a culture medium for producing carrageenase by using fermentation of edible pelvetia silquosa pseudoalteromonas (Pseudoalteromonas sp.) ASY5. The culture medium comprises a strain activation medium, a seed medium and a fermentation medium. The invention also discloses a fermentation method for producing the carrageenase by using fermentation of edible pelvetia silquosa pseudoalteromonas. On the basis of shake-flask fermentation, the screened edible pelvetia silquosa pseudoalteromonas is subjected to fermentation cultivation in a fermentation tank. The enzyme producing rule of the pseudoalteromonas in the fermentation tank is researched, the yield of the carrageenase is effectively improved, and the carrageenase produced by using fermentation of edible pelvetia silquosa pseudoalteromonas (Pseudoalteromonas sp.) ASY5 is subjected to pilot plant test scale-up fermentation, and important technical parameter guidance is provided for large-scale production in the future.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a medium for producing carrageenase by fermentation of Pseudoalteromonas carrageenans and a fermentation method thereof. Background technique [0002] Carrageenan is a natural seaweed polysaccharide extracted from the cell walls of some red algae. It is widely used in biochemical, clinical, pharmaceutical, food, chemical and other fields. Carrageenan oligosaccharide is formed by the degradation of carrageenan. It has various new physiological activities such as anti-oxidation, anti-virus, anti-tumor, immune regulation and promotion of plant growth. Compared with carrageenan, it has a wider application value. [0003] At present, karaoligosaccharides are generally obtained through traditional chemical and physical degradation methods, but the traditional methods of preparing karaoligosaccharides have problems such as difficult control of reaction conditions, uneven degradatio...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12R1/01
CPCC12N9/2468
Inventor 肖安风蔡慧农倪辉许彩云朱艳冰杨秋明陈艳红姜泽东杜希萍杨远帆黄高凌李利君
Owner JIMEI UNIV
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