Preparation and application method of special microorganism composite bacterial agent for directly decomposing and fermenting crop straws to generate marsh gas
A technology of crop straw and compound bacterial agent, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large amount of biogas production, low treatment cost, shortening of initial gas production time, etc.
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Embodiment 1
[0046] Embodiment one, preparation of special microbial solid bacterial agent
[0047] Fermentation culture of bacterial strains
[0048] No. 1-7 bacterial classifications are carried out rejuvenation and ultraviolet mutagenesis according to conventional methods respectively (above technology is well known to those skilled in the art), respectively adopt the following conditions for fermentation and cultivation separately: culture medium is bacterial nutrient broth culture medium, purchased from Qingdao Haibo Biotechnology Co., Ltd. prepared 10 L according to the instructions, pH 7.2, and put it into an 18-liter automatic fermenter; the culture conditions were: culture temperature 30 ° C; stirring speed 200 rpm; sterile air ventilation rate 1:1; The culture time is 24 hours; the concentration of spores at the end of fermentation is >1×109 / ml.
[0049] No. 8-12 strains were separately fermented and cultured under the following conditions: the culture medium was bacterial anaer...
Embodiment 2
[0055] Embodiment two, the preparation method of microbial liquid bacterial agent
[0056] Fermentation culture of bacterial strains
[0057] No. 1-7 bacterial classifications are carried out rejuvenation and ultraviolet mutagenesis according to conventional methods respectively (above technology is well known to those skilled in the art), respectively adopt the following conditions for fermentation and cultivation separately: culture medium is bacterial nutrient broth culture medium, purchased from Qingdao Haibo Biotechnology Co., Ltd. prepared 10 L according to the instructions, pH 7.2, and put it into a 16-liter automatic fermenter; the culture conditions were: culture temperature 30 ° C; stirring speed 200 rpm; sterile air ventilation 1:1; The culture time is 24 hours; the concentration of spores at the end of fermentation is >1×109 / ml.
[0058] 8-12 bacterial strains were separately adopted the following fermentation culture conditions: the culture medium was bacterial a...
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