Preparation and application method of special microorganism composite bacterial agent for directly decomposing and fermenting crop straws to generate marsh gas

A technology of crop straw and compound bacterial agent, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of large amount of biogas production, low treatment cost, shortening of initial gas production time, etc.

Inactive Publication Date: 2019-07-02
王旭宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a preparation of a special microbial composite bacterial agent that directly decomposes crop stalks for fermentation to produce biogas, and simultaneously provides an application method for utilizing the microbial composite bacterial agent to decompose crop stalks for processing and fermentation to produce biogas; it effectively overcomes the In the prior art, the fermentation is not good, which affects the production and yield of biogas. The crop stalks treated with microbial agents have a fast fermentation speed and a large amount of biogas, whic

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one, preparation of special microbial solid bacterial agent

[0047] Fermentation culture of bacterial strains

[0048] No. 1-7 bacterial classifications are carried out rejuvenation and ultraviolet mutagenesis according to conventional methods respectively (above technology is well known to those skilled in the art), respectively adopt the following conditions for fermentation and cultivation separately: culture medium is bacterial nutrient broth culture medium, purchased from Qingdao Haibo Biotechnology Co., Ltd. prepared 10 L according to the instructions, pH 7.2, and put it into an 18-liter automatic fermenter; the culture conditions were: culture temperature 30 ° C; stirring speed 200 rpm; sterile air ventilation rate 1:1; The culture time is 24 hours; the concentration of spores at the end of fermentation is >1×109 / ml.

[0049] No. 8-12 strains were separately fermented and cultured under the following conditions: the culture medium was bacterial anaer...

Embodiment 2

[0055] Embodiment two, the preparation method of microbial liquid bacterial agent

[0056] Fermentation culture of bacterial strains

[0057] No. 1-7 bacterial classifications are carried out rejuvenation and ultraviolet mutagenesis according to conventional methods respectively (above technology is well known to those skilled in the art), respectively adopt the following conditions for fermentation and cultivation separately: culture medium is bacterial nutrient broth culture medium, purchased from Qingdao Haibo Biotechnology Co., Ltd. prepared 10 L according to the instructions, pH 7.2, and put it into a 16-liter automatic fermenter; the culture conditions were: culture temperature 30 ° C; stirring speed 200 rpm; sterile air ventilation 1:1; The culture time is 24 hours; the concentration of spores at the end of fermentation is >1×109 / ml.

[0058] 8-12 bacterial strains were separately adopted the following fermentation culture conditions: the culture medium was bacterial a...

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PUM

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Abstract

The invention discloses preparation and an application method of a special microorganism composite bacterial agent for directly decomposing and fermenting crop straws to generate marsh gas. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing of one or more of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaericus, clostridium sporogenes, clostridium acetobutylicum, clostridium pasteurianum, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichoderma pseudokoningii and aspergillus niger. Meanwhile, by taking the special microorganism composite bacterial agent as an inoculation bacterial agent,the invention provides the application method for decomposing the crop straws and accelerating the fermentation to generate marsh gas; the crop straws treated by the microorganism composite bacterialagent have fast fermentation speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no any faeces of poultry and livestock, and the application range is quite wide.

Description

technical field [0001] The invention relates to the preparation and application method of a special microbial compound bacterial agent for directly decomposing crop straws to ferment and produce biogas, especially the preparation of a microbial bacterial agent for decomposing cellulosic crop straws; and using the special compound bacterial agent as an inoculum It provides an application method for decomposing crop stalks and accelerating fermentation to produce biogas. At the same time, it can also be used for biological treatment of other organic solid wastes to produce biogas. Background technique [0002] In order to improve the rural ecological environment and improve the quality of life of farmers, the state vigorously promotes the production and utilization of clean energy-biogas. At present, my country’s rural areas mainly use livestock and poultry manure to produce biogas. According to statistics, due to the uneven number of livestock and poultry raised by each farm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P5/02C12R1/085C12R1/125C12R1/10C12R1/11C12R1/07C12R1/09C12R1/145C12R1/885C12R1/685
CPCC12N1/14C12N1/20C12P5/023C12P2201/00C12P2203/00Y02E50/30
Inventor 王旭宁
Owner 王旭宁
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