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Special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof

A technology of crop straw and compound bacterial agent, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low processing cost, large amount of biogas production, and shortening of initial gas production time.

Inactive Publication Date: 2010-07-14
刘相梅 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a preparation of a special microbial composite bacterial agent that directly decomposes crop stalks for fermentation to produce biogas, and simultaneously provides an application method for utilizing the microbial composite bacterial agent to decompose crop stalks for processing and fermentation to produce biogas; it effectively overcomes the In the prior art, the fermentation is not good, which affects the production and yield of biogas. The crop stalks treated with microbial agents have a fast fermentation speed and a large amount of biogas, which shortens the initial gas production time by more than 50% and increases the biogas production by 30%. It can make all corn stalks, rice straws, wheat stalks and other crop stalks used as raw materials for biogas fermentation. Compared with other existing treatment methods, the treatment cost is low, and it can be treated in farmyards, fields, and beside biogas tanks. And there is no secondary pollution; after the straw is treated, it can be fermented in a biogas digester to produce biogas, and it can also produce biogas without any poultry and livestock manure, and the scope of application is very wide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Special microbial solid bacteria preparation

[0047] Bacterial bacteria fermentation culture

[0048] Putting the bacteria No. 1-7 in accordance with the conventional methods, the recurrence and ultraviolet mutation (the above technology is known to the professionals in the field), and the following conditions are fermented and cultivated separately: the medium is based on the bacterial nutrition soup culture medium, and the purchase is purchased from Qingdao from Qingdao Haibo Biotechnology Co., Ltd. is made into 10L and pH7.2 according to the instructions, and the 18 -liter automatic fermentation tank is loaded; the training conditions are: the cultivation temperature 30 ° C; the stirring speed 200 rpm is 200 rpm; The cultivation time is 24 hours; the spore concentration at the end of the fermentation> 1 × 10 9 One / ml.

[0049] Bacterium 8-12 uses the following conditions to ferment culture separately: The medium is based on bacterial anaerobic pillar medium, ...

Embodiment 2

[0055] Example II. Preparation method of microbial liquid bacteria agent

[0056] Bacterial bacteria fermentation culture

[0057] Putting the bacteria No. 1-7 in accordance with the conventional methods, the recurrence and ultraviolet mutation (the above technology is known to the professionals in the field), and the following conditions are fermented and cultivated separately: the medium is based on the bacterial nutrition soup culture medium, and the purchase is purchased from Qingdao from Qingdao Haibo Biotechnology Co., Ltd. is made into 10L and pH7.2 according to the instructions, loaded into 16 -liter automatic fermentation tanks; the cultivation conditions are: training temperature 30 ° C; 200 rpm of stirring speed; 1: 1 of sterile air ventilation; 1: 1; The training time is 24 hours; the spore concentration at the end of the fermentation is> 1 × 10 9 One / ml.

[0058] 8-12 Bacterial species use the following fermentation culture conditions separately: The medium is based o...

Embodiment 3

[0063] Example III. Use a special microbial composite agent (solid state) to decompose crop straws to decompose and treat fermented biogas

[0064] The application selects 8 cubic meters to use the biogas.

[0065] 1. Cut or crushed: Straight straw with thick stalks such as corn is crushed with multi -functional machines, and the softer straws such as straw and other stems are cut short or crushed to less than 6cm. Rong needs 50kg of straw, crushing 400kg of corn straw.

[0066] 2. Moisture and inoculation of straw: Make urea water with 2.5kg urea against 180kg of water and sprinkle it evenly on the straw pile. It is advisable to scatter the water without dripping water); after the urea water is soaked, it is covered with breathable coverage on it, and naturally placed for 24 hours, and then the amount of microbial composite agglomerate for 1kg of stalks is added to The stacked straw is sprinkled while turning.

[0067] 3. Packing: Stir stacking straw into strip stacks, 1.5m wide,...

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Abstract

The invention discloses a special microorganism composite bacterial agent for directly decomposing and fermenting crops straws to generate marsh gas and application method thereof. The special microorganism composite bacterial agent is liquid or solid, and is formed by fermenting, culturing, combining and compositing one or a plurality of bacillus cereus, bacillus subtilis, bacillus licheniformis, bacillus megaterium, bacillus mucilaginosus, bacillus circulans, bacillus sphaerieus, clostridium sporogenes, clostridium acetobutylicum, clostridium barati, clostridium beijerinckii, clostridium thermocellum, trichoderma viride, trichodermareesei and aspergillus niger. Simultaneously, by taking the special microorganism composite bacterial agent as a vaccination bacterial agent, the invention provides an application method for decomposing the crops straws and accelerating the ferment to generate marsh gas; and the microorganism composite bacterial agent-treated crops straws have fast ferment speed, large marsh gas generation quantity, low treating cost and no secondary pollution, can be fermented to generate the marsh gas under the condition of no faeces of poultry, and have very wide application range.

Description

Technical field [0001] The present invention involves a special microbial composite bacteria preparation and its application method that directly decomposes crop straw fermentation biogas, especially the preparation of microbial bacteria agents that decomposes the decomposition of cellulose crop straw; The agent provides a decomposition treatment of crop straws, and the application method of accelerating the biogas of fermentation. It can also be used to treat biogas in biogas to other organic solid wastes. Background technique [0002] In order to improve the rural ecological environment and improve the quality of life of farmers, the state vigorously promote the production and utilization of clean energy-biogas. At present, the countryside in my country mainly uses biogas and poultry feces. According to statistics, due to the uneven number of farmers and poultry, the number of farmers breeding in different seasons is large. The residue and biogas of biogas are good organic fert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P5/02C12R1/685C12R1/885C12R1/01C12R1/145C12R1/07C12R1/09C12R1/11C12R1/10C12R1/125C12R1/085
CPCY02E50/343Y02E50/30Y02T50/678
Inventor 刘相梅祝捷
Owner 刘相梅
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