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GY2B degrading bacteria immobilizing ball as well as preparation method and application thereof

A technology of immobilizing small balls and degrading bacteria, applied in the field of microbial degradation of organic pollutants, can solve the problems of loss and degradation, troublesome, easy death of microorganisms, etc. Effect

Active Publication Date: 2015-05-06
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is very easy for microorganisms to die, and the loss will cause troubles for degradation treatment. Therefore, the use of immobilized microorganisms to deal with water pollution has gradually attracted attention and application.

Method used

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  • GY2B degrading bacteria immobilizing ball as well as preparation method and application thereof
  • GY2B degrading bacteria immobilizing ball as well as preparation method and application thereof
  • GY2B degrading bacteria immobilizing ball as well as preparation method and application thereof

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Embodiment 1

[0050] A GY2B degrading bacteria immobilized pellet, the preparation method of which is:

[0051] (1) Preparation of GY2B-degrading bacteria suspension: pick one to two rings from the activated GY2B-degrading bacteria colonies that have been subcultured for 3 to 5 times to enrich the culture medium containing 100 mg / L phenol concentration, and place in a constant temperature shaking incubator Cultivate for 12 hours to obtain a concentrated bacterial solution containing a large amount of bacteria; centrifuge and wash the concentrated bacterial solution with sterile physiological saline for 3 times at a centrifugation temperature of 4°C, a centrifugal speed of 8000r / min, and a centrifugation time of 15 minutes; the washed bacteria Suspended in normal saline, adjust the concentration of the bacterial solution at 600nm under the UV spectrophotometer, so that the absorbance value is A=1, at this time the concentration of the GY2B-degrading bacteria in the GY2B-degrading bacteria sus...

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Abstract

The invention belongs to the technical field of microbial degradation of organic pollutants, and discloses a GY2B degrading bacteria immobilizing ball as well as a preparation method and application thereof. The GY2B degrading bacteria immobilizing ball is a gel ball formed by embedding GY2B degrading bacteria by taking a crosslinking polymerization product of polyvinyl alcohol, algin and kaolin as a carrier. The preparation method disclosed by the invention comprises the following steps: (1), sequentially adding polyvinyl alcohol and algin into water, heating, stirring and dissolving respectively, adding kaolin, and stirring and mixing uniformly so as to obtain a mixed solution; (2), cooling the mixed solution to room temperature under irradiation of ultraviolet light, adding a GY2B degrading bacteria suspension, and uniformly stirring to obtain a bacteria embedding solution; and (3), dropwise adding the bacteria embedding solution into a crosslinking solution, and crosslinking, solidifying and condensing to obtain the GY2B degrading bacteria immobilizing ball. The GY2B degrading bacteria immobilizing ball has more thorough, rapid and steady degrading effects to phenol; furthermore, a steady degrading environment can be provided; and the GY2B degrading bacteria immobilizing ball is adaptive to a toxic environment.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation of organic pollutants, and relates to a GY2B degrading bacteria immobilized pellet and a preparation method and application thereof. Background technique [0002] In response to the development of economy and society, phenol is an important organic raw material, which is widely used in medicine, resin, sterilizer and many other aspects. However, its corrosiveness, toxicity, and accumulation make it a great threat to the human body, the atmosphere and water in the environment. The method of microbial treatment of organic pollutants has been paid attention to and used for its low cost, small secondary pollution and complete degradation. At present, people mostly adopt the method of directly adding microecological preparations to water bodies for organic pollutants including phenol in water bodies. However, this method is very easy for microorganisms to die, and the loss will cause tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08C02F3/34C02F101/38
Inventor 吴平霄李跃武党志朱能武
Owner SOUTH CHINA UNIV OF TECH
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