Rhodococcus sp.QY-2 having low-temperature petroleum degrading function and application thereof

A technology of rhodococcus and petroleum, applied in the field of microorganisms, can solve the problems of ecological system hazards, slow evaporation of short-chain petroleum hydrocarbons, increase the difficulty of oil spill emergency and ecological restoration work, and achieve simple cultivation methods and petroleum hydrocarbon degradation capabilities strong effect

Active Publication Date: 2016-08-31
DALIAN MARITIME UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In winter, especially during the glacial period, once an oil spill accident occurs at sea, the short-chain petroleum hydrocarbons evaporate slowly, and the long-chain hydrocarbons condense into solids, making or

Method used

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  • Rhodococcus sp.QY-2 having low-temperature petroleum degrading function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of strain Rhodococcus sp.QY-2

[0020] 1. Isolation of strain Rhodococcus sp.QY-2

[0021] (1) Sample collection: On November 22, 2013, the oil pipeline of the Weifang Branch of Sinopec Oil Transportation, Storage and Transportation Company ruptured, and some crude oil polluted the waters near Jiaozhou Bay. One week after the oil spill, take the seawater contaminated by crude oil on the surface;

[0022] (2) Enrichment acclimation: add 100 mL of MMC medium to a 250 mL conical flask, sterilize at 120° C. for 15 min, and add 5 mL of collected seawater samples and 0.5 mL of sterilized crude oil. Put the mixture into a biochemical incubator, and shake it at 150 r / min and 10 °C for 30 days; after the incubation, take 5 mL of the culture solution for the next round of transfer culture: add 5 mL of the culture solution to the sterilized crude oil containing 0.5 mL. In 100 mL of MMC medium, cultured at 150 r / min for 30 d. The above step...

Embodiment 2

[0027] Example 2 Fermentation of strain Rhodococcus sp.QY-2

[0028] Pick the purified fungus with a sterile inoculating loop and put it into a conical flask containing 100 mL of sterilized 2216E liquid medium, and shake it at low temperature for 7 days (0°C, 120 r / min) to become the seed liquid; The fermented liquid was added to 2216E liquid medium at a volume ratio of 10%, and cultured at 0° C. for 7 days to obtain a fermentation broth.

Embodiment 3

[0029] Example 3 Determination of petroleum hydrocarbon degradation rate of strain Rhodococcus sp.QY-2 at 0°C

[0030] (1) Degrading bacteria seed liquid

[0031] Pick the purified bacterial lawn with a sterile inoculating loop into a conical flask containing 100 mL of sterilized 2216E liquid medium, and culture with shaking at low temperature for 7 days (0°C, 120 r / min).

[0032] (2) Degradation culture

[0033] Weigh 0.5 g of crude oil and dissolve it in 20 mL of filtered sterilized n-hexane, transfer it to a conical flask containing 100 mL of sterilized MMC liquid medium, and place it on a shaker to shake until the n-hexane is completely evaporated. The seed solution of strain Rhodococcus sp. QY-2 was inoculated with 10% of the inoculum, and cultured with shaking at low temperature for 60 d (0° C., 120 r / min). The culture medium without degrading bacteria was used as blank group.

[0034] (3) Extraction

[0035] After the degradation culture, 3 mL of 50% sulfuric acid s...

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Abstract

The invention discloses Rhodococcus sp.QY-2 having a low-temperature petroleum degrading function and collected under CGMCC No.11634. The Rhodococcus sp.QY-2 is screened from a sea area polluted by petroleum in North China, can efficiently degrade petroleum at low temperature, especially in extreme environment of 0 DEG C. The Rhodococcus sp.QY-2 can be applied in bioremediation of sea petroleum pollution, especially in petroleum spilling bioremediation at low temperature in the sea area in North China in winter (including glacial period).

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a marine low temperature bacteria and its application in the bioremediation of marine oil pollution. The strain has the characteristics of being able to efficiently degrade petroleum hydrocarbons under extreme conditions of 0°C. Background technique [0002] With the rapid development of the marine transportation industry and the rapid rise of the marine mining industry, the occurrence of marine oil spill accidents has become more and more frequent, which has seriously damaged the balance of the marine environment. In winter, especially during the glacial period, once a marine oil spill accident occurs, due to the slow evaporation of short-chain petroleum hydrocarbons and the condensation of long-chain hydrocarbons into solids, organic pollutants will persist, which will cause considerable harm to the local ecosystem, and also It has increased the difficulty of oil spill re...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
CPCC02F3/344C02F2101/32C12N1/205C12R2001/01
Inventor 郭平林建国曹宾霞
Owner DALIAN MARITIME UNIVERSITY
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