Method for fixing oil decomposing bacteria by polyurethane foam

Abstract

The invention discloses a method for fixing oil decomposing bacteria by polyurethane foam and relates to fixation of a group of oil decomposing bacteria. The method for fixing oil decomposing bacteria by polyurethane foam can increase the oil decomposition rate. The method comprises the following steps of: pelletizing the polyurethane foam, boiling the polyurethane foam in boiled water, removing polyether type substances which are contained in the polyurethane foam and harmful to cells, drying the polyurethane foam, sterilizing the polyurethane foam together with a 2216 culture medium, and inoculating the polyurethane foam in the oil decomposing bacteria for shake-flask culture so that the oil decomposing bacteria enters or is adsorbed on the inner wall of the polyurethane foam to grow in order to form polyurethane foam with high-density oil decomposing bacteria. According to the invention, polyurethane foam with certain density and size, free of tox and pollution, good in mass transfer effect and energy transfer effect and also convenient and low-cost is adopted to fix the oil decomposing bacteria, so that the application performance in actual sea oil pollution abatement is remarkably improved, and the used polyurethane foam can be recycled after special treatment, therefore good economic benefit is achieved.

Description

technical field [0001] The invention relates to the immobilization of a group of petroleum-degrading bacteria, in particular to a method for immobilizing petroleum-degrading bacteria by using polyurethane foam as a carrier, so as to resist the interference of washing and dilution of seawater and improve the degradation ability of petroleum. Background technique [0002] With the rapid development of the global economy and the surge in population, the ocean has played an important role in the sustainable development of mankind. However, with the development and use of marine resources, the ocean has also been seriously polluted, among which oil pollution is particularly prominent. Offshore oil and its product pollution is one of the most important ecologically catastrophic pollution. Coastal offshore oil exploitation and pipelines, as well as merchant shipping are potential sources of mobile pollution. Oil will form an oil film on the sea surface, which isolates the air flow...

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Problems solved by technology

At present, domestic and foreign research on this aspect is still in its infancy ([13] Cui Zhisong, Zheng Li, Yang Baijuan, Liu Qian, Gao Wei. The synergistic effect of two marine obligate hydrocarbon decomposing bacteria on oil degradation[J]. Acta Microbiology, 2010,50(3):350-359; [14]Wang,H.,Xu,R.,Li,F.Efficient degradation of lube oil by a mixed bacterial consortium[J].Journal of Environmental Sciences-China,2010, 22(3):381-388.), the construction of oil-degrading bacteria is limited to simple mixing, lacking a certain theoretical basis

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