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Microorganism composite bacterial preparation, and preparation method and applications thereof

A compound bacterial agent and microorganism technology, applied in the field of environmental microorganisms, can solve the problems of low removal efficiency, incomplete degradation of aromatic hydrocarbons, poor environmental adaptability and functional stability, etc., achieving good stability and breaking industry technical barriers and equipment requirements. brief effect

Inactive Publication Date: 2018-06-26
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microorganisms used to degrade petroleum components in oily wastewater have the disadvantages of low removal efficiency, incomplete degradation of aromatic hydrocarbons, poor environmental adaptability and functional stability, etc.

Method used

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  • Microorganism composite bacterial preparation, and preparation method and applications thereof
  • Microorganism composite bacterial preparation, and preparation method and applications thereof
  • Microorganism composite bacterial preparation, and preparation method and applications thereof

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preparation example Construction

[0042] The preparation method of the above-mentioned microbial composite bacterial agent will be further described below, and the preparation method of the microbial composite bacterial agent comprises:

[0043] S1, the first activation culture

[0044] Specifically, the first activation culture is to inoculate Dieselophane, Pseudomonas spongiosa, Acinetobacter venezius and M. venezii into the liquid culture medium (liquid HLB) of the activated strains for cultivation, that is, -70°C The preserved glycerol tube strains of the above four strains were respectively inoculated into 15mL culture tubes containing 2-5mL activated strain liquid medium, and the culture tubes were placed in a shaking shaker at 20-28°C and 180-220r / min Incubate for 48-120 hours.

[0045] Wherein, the activated strain liquid medium includes tryptone 8-12g / L, yeast extract 4-6g / L, sodium chloride 25-35g / L and deionized water; the pH value is 6-7.5.

[0046] S2, the second activation culture

[0047] The...

Embodiment 1

[0062] Firstly, Dieselophane with the preservation registration number 1A00001, Pseudomonas spongiosa with the preservation registration number 1A00102 and the preservation registration number 1A00294 obtained from the China Marine Microorganism Culture Collection Management Center (MCCC) were stored at -70°C. The glycerol tube strains of Acinetobacter venezius and Marinococcus dehydrocarbons with deposit registration number 1A03971 were respectively inoculated into 15mL culture tubes containing 5mL liquid HLB, and the culture tubes were placed on a shaking shaker at 28°C and 220r / min. cultured for 120 hours. Then, the obtained bacterial liquid was streaked in the solid medium of the activated strain and placed in a constant temperature incubator at 28°C for rejuvenation for 96 hours, and the colonies on the plate were each inoculated into 15mL culture tubes containing 5mL of liquid HLB and placed at 28°C. Incubate overnight in a shaking shaker at 200r / min.

[0063] Secondly,...

Embodiment 2

[0067] Firstly, Dieselophane with the preservation registration number 1A00001, Pseudomonas spongiosa with the preservation registration number 1A00102 and the preservation registration number 1A00294 obtained from the China Marine Microorganism Culture Collection Management Center (MCCC) were stored at -70°C. The glycerol tube strains of Acinetobacter venezius and Marinococcus dehydrocarbons with deposit registration number 1A03971 were respectively inoculated into 15mL culture tubes containing 2mL liquid HLB, and the culture tubes were placed on a shaking shaker at 20°C and 180r / min. Incubate for 48 hours. Then, the obtained bacterial liquid was streaked in the solid medium of the activated strain and placed in a constant temperature incubator at 20°C for rejuvenation for 24 hours. The colonies on the plate were each inoculated into 15mL culture tubes containing 2mL liquid HLB and placed at 20°C. Cultured overnight in a shaking shaker at 180r / min.

[0068] Secondly, inocula...

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Abstract

The invention discloses a microorganism composite bacterial preparation, and a preparation method and applications thereof, and belongs to the field of environment microorganism. The microorganism main component of the microorganism composite bacterial preparation comprises Alcanivorax dieselolei, Pseudomonas pachastrellae, Acinetobacter venetianus, and Marinobacter hydrocarbonoclasticus; the microorganism composite bacterial preparation can be used for degrading petroleum hydrocarbons, and degrading petroleum hydrocarbons in sewage, is capable of realizing comprehensive effective degradationof the large amounts of straight chain alkanes, branched-chain hydrocarbons, and aromatic hydrocarbons in petroleum hydrocarbons, is high in removing rate, is capable of solving a sea petroleum pollution pollution environment problem through degradation of petroleum hydrocarbons. The microorganism composite bacterial preparation is prepared via inoculation of a bacterial preparation culture mediumwith the Alcanivorax dieselolei, Pseudomonas pachastrellae, Acinetobacter venetianus, and Marinobacter hydrocarbonoclasticus, and co-culturing; the preparation process is simple; equipment requirements are low; operation is simple; needed environment conditions are mild; and industrial popularization and applications are convenient to realize.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a microbial composite bacterial agent and its preparation method and application. Background technique [0002] Most of the oil pollution occurs in the marine environment, which will cause very serious harm to the ocean and its surrounding ecological environment. It will not only cause a lack of oxygen in large areas of the sea, resulting in the death of marine animals and plants, but also cause deserted beaches, damage to mariculture and salt pans production, destroying coastal tourist areas, and reducing the service function and value of the marine ecosystem; in addition, many petroleum hydrocarbons enter the fish, shrimp and shellfish through the food chain, affecting their economic value, and enter the human body through food enrichment, endangering human health. Therefore, oil pollution is the greatest threat to the marine ecological environment. [0003] The com...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/38C02F103/08C02F101/32
CPCC02F3/343C02F2101/32C02F2103/08C12N1/20
Inventor 刘陈立何彩云何敬愉张炳照傅雄飞魏婷
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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