Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for screening and domesticating marine oil pollutant microorganisms and use of marine oil fouling microorganisms

A technology for microbial flora and oil pollution, applied in biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of long domestication time, cumbersome screening process, insufficient salinity adaptation, etc. low effect

Inactive Publication Date: 2012-12-26
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current screening of marine oil-degrading bacteria, there are mainly problems such as long domestication time, insufficient salinity adaptation and cumbersome screening process.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for screening and domesticating marine oil pollutant microorganisms and use of marine oil fouling microorganisms
  • Method for screening and domesticating marine oil pollutant microorganisms and use of marine oil fouling microorganisms
  • Method for screening and domesticating marine oil pollutant microorganisms and use of marine oil fouling microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] see figure 1, a device for simulating bioremediation of beach oil spills, mainly composed of a cylinder 11, a partition 3, a right thermometer 4, a left thermometer 5, a wave maker 6, a temperature control device 7 and a lighting device 8, the two sides of the bottom of the cylinder 11 There is a right water outlet 1 and a left water outlet 9, and correspondingly a right water valve 2 and a left water valve 10, and a detachable partition 3 is arranged in the middle of the cylinder to separate the cylinder 11 into two left and right Space, the left thermometer 5 and the right thermometer 4 are respectively installed on the left and right sides of the cylinder body, and the readings of the thermometers can be observed from the outside. The detachable VP-201 artificial wave maker 6 and wave maker 6 are installed on the right side of the cylinder body 11 The inner wall of the cylinder is sucked by the suction cup, and the HT-75W temperature control device 7 is also fixed by...

Embodiment 2

[0050] (1) Bacterial source sample acquisition:

[0051] Water samples are collected in sterile blue-necked bottles of about 500ml of water samples in the static water layer. Sludge samples are collected from the surface of oil sludge about 0.5-1.0kg and placed in sterile containers. All samples should be enriched within 24 hours. Collected culture or sealed storage at 4 ℃.

[0052] The study found that the marine oil pollution occurred and the surrounding areas where marine oil pollution accidents occurred before are rich in resources of oil pollution degrading bacteria, and these bacteria bear the heavy responsibility of eliminating marine oil pollution. The bacteria source screening samples of the present invention come from oily waste water and sludge produced after cleaning ships at Zhoushan Wharf. These wastes contain high salinity and a layer of oil slick floats on the upper layer.

[0053] (2) Bacteria enrichment:

[0054] Take 10ml of water sample and 0.5g of sludge...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for screening and domesticating marine oil pollutant microorganisms, which comprises: acquiring a microorganism source sample in a marine oil polluted area; performing enrichment culture of one generation, second generation and third generation to obtain a culture solution; screening, enriching and performing enrichment culture of one generation, second generation and third generation, obtaining an enriched culture solution; and adding nutritional materials into a designed device to domesticating the microorganisms and obtaining domesticated microorganisms. The screened microorganisms can be used for degrading oil pollutant in areas where marine oil pollution accidents happen, and can be for biological remediation of oil overflowing coastal beach and petroleum degradation. The method has the characteristics of high speed, economy, high efficiency and environment friendliness, can be used quickly for handling marine oil pollution accidents and is an ideal choice for screening and domesticating marine oil pollutant microorganisms.

Description

technical field [0001] The invention belongs to the field of seawater treatment, and relates to a method for screening and domesticating marine oily microbial flora, which is suitable for bioremediation and biological treatment of marine oily wastewater and sludge. Background technique [0002] Petroleum is an invaluable fuel, lubricant and chemical raw material. With the rapid development of economy and society, oil and its products are widely used in various fields of the national economy and in the daily life of human beings, and their usage is increasing day by day. With the world's increasing demand for oil and its products, oil spill accidents are increasing in the process of offshore mining, transportation, loading and unloading, and utilization of oil, causing a large amount of oil to enter water bodies and flow into the ocean. Oil pollutants have become one of the main pollutants in the ocean. After oil enters the ocean water body, it will cause serious damage to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C02F3/34C02F103/08
Inventor 郑刚杨志坚张文武肖金星
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com