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Exiguobacterium marinum and microbial inoculant, and application thereof

A technology of microbial agent, microbacteria marinum, applied in the field of biology, can solve the problem of low rate of petroleum hydrocarbons

Inactive Publication Date: 2014-07-23
烟台大境生态环境科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the rate of degradation of petroleum hydrocarbons by existing microbial strains is relatively low, the present invention provides a kind of Exiguobacterium marinum, and the preservation number of this Exiguobacterium marinum is CGMCC NO.8841

Method used

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  • Exiguobacterium marinum and microbial inoculant, and application thereof
  • Exiguobacterium marinum and microbial inoculant, and application thereof

Examples

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preparation example Construction

[0019] Wherein, the preparation method of the microbial inoculum may include: inoculating Microbacterium marinum in the culture medium to cultivate, so that the total number of live bacteria per gram of the microbial inoculum obtained is (2-300)×10 8 CFU. The temperature of cultivation may be 28°C.

[0020] Wherein, in order to further improve the effect of the microbial bacterial agent degrading petroleum hydrocarbons, preferably, the preparation method of the microbial bacterial agent may also include: mixing the material obtained after culturing in the fermentation medium with nutrient salts; Nutrients include NaBr, H 3 BO 3 , SrCl 2 , NaF, potassium sodium tartrate and trisodium citrate. More preferably, the consumption of nutrient salt makes every kilogram of described microbial bacterial agent contain the NaBr of 50-100mg, the H of 10-40mg 3 BO 3 , 10-40mg of SrCl 2 , 1-5mg of NaF, 0.5-5g of potassium sodium tartrate and 10-100mg of trisodium citrate.

[0021] Th...

Embodiment 1

[0026] This example is used to illustrate the cultivation of Microbacterium marinum and the preparation of microbial inoculum of the present invention.

[0027] Inoculate Exiguobacterium marinum (Exiguobacterium marinum) with preservation number CGMCC NO.8841 into LB medium (containing 10g / L of tryptone, 5g / L of yeast extract, 10g / L of NaCl and the rest of the water) , cultured to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0028] Add 100mL of the above-mentioned seed bacteria liquid into 100L fermentation medium (the fermentation medium contains 80g / L soybean cake powder, 3g / L sodium chloride, 3g / L calcium carbonate, 5g / L potassium dihydrogen phosphate and 5g / L L of ferrous sulfate), cultivated at 30°C, sampled during the cultivation and observed by direct microscope counting until the number of viable microbacteria per gram of culture solution was 10. 9 CFU, the resulting culture solution is the microbial agent of this embodi...

Embodiment 2

[0030] This example is used to illustrate the cultivation of Microbacterium marinum and the preparation of microbial inoculum of the present invention.

[0031] Inoculate Exiguobacterium marinum (Exiguobacterium marinum) with preservation number CGMCC NO.8841 into LB medium (containing 10g / L of tryptone, 5g / L of yeast extract, 10g / L of NaCl and the rest of the water) , cultured to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0032] Add 100mL of the above-mentioned seed bacteria liquid into 100L fermentation medium (the fermentation medium contains 80g / L soybean cake powder, 3g / L sodium chloride, 3g / L calcium carbonate, 5g / L potassium dihydrogen phosphate and 5g / L L of ferrous sulfate), cultivated at 30°C, sampled during the cultivation and observed by direct microscope counting until the number of viable microbacteria per gram of culture solution was 10. 9 CFU, add 80mg of NaBr, 30mg of H 3 BO 3 , 20mg of SrCl 2 , 3mg of NaF,...

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Abstract

The invention provides a Exiguobacterium marinum of which the collection number is CGMCC No.8841. The invention also provides a microbial inoculant which comprises a culture medium and a thallus, wherein the thallus comprises the Exiguobacterium marinum of which the collection number is CGMCC No.8841. The invention also provides application of the Exiguobacterium marinum and the microbial inoculant in degradation of petroleum hydrocarbons. The technical scheme of the invention can enhance the ocean petroleum pollution treatment efficiency more effectively.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microbacterium marinum, a microbial agent and applications thereof. Background technique [0002] The frequent occurrence of marine oil spills not only causes huge economic losses to the marine aquaculture and tourism industries, but also has long-term negative effects on the marine ecological environment and human health. Therefore, how to eliminate marine oil spill pollution and restore damaged marine ecological environment has become one of the important contents of marine environmental protection. With the development of offshore oil mining and transportation, the problem of marine oil pollution is becoming more and more serious. For example, the overturning of drilling platforms, the bursting of oil pipelines or the wreck of oil tankers will cause a large amount of oil to leak into the marine environment. If scientific and effective countermeasures are not taken to remove it,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/01A62D101/20C02F101/32C02F103/08
Inventor 赵文勇阮志勇赵秉强王慧敏赵君才吕昭辉刘慧宋昭王彦伟刘小飞
Owner 烟台大境生态环境科技股份有限公司
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