Method for removing aerosol pollution in laboratory

Abstract

The invention relates to a method for removing aerosol pollution in a laboratory. The method comprises the following steps of spraying 0.01-0.2% of 84 disinfectant on inner walls, ground, an experiment table top, experiment instruments, vessels, consumables and the like of the laboratory, and spraying 50-75% of alcohol 5-10 minutes later. The method is used for preventing pollution and can be usedafter each laboratory is finished. If the method is used in a polluted laboratory, the method is repeatedly used once every other day and continuously used for three times, so that aerosol pollutionin the laboratory can be thoroughly removed. The method is simple to operate and extremely low in cost, and the problem of the aerosol pollution can be thoroughly solved in one week in the laboratorywith serious pollution.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a method for removing laboratory aerosol pollution. Background technique [0002] Since the advent of PCR technology in the 1980s, it has been widely used in many life science-related fields, such as biology, animal medicine, medicine, forensic science, botany, etc., and has become one of the essential basic technologies for the study of life sciences. Especially fluorescent PCR technology, its sensitivity is more than 100 times higher than that of ordinary PCR technology, and the amplification process is more than twice as short as that of ordinary PCR. From the principle of PCR technology, it can be known that as long as there is one DNA or RNA molecule as a template initially, billions of molecules with the same base sequence as the original template will be produced after numerous amplification reactions. Amplification continues as template alone. If these amplified product...

AI Technical Summary

Problems solved by technology

If these amplified products are accidentally leaked into the laboratory environment during the operation, the laboratory environment will be polluted, which is called aerosol pollution. After the laboratory is contaminated, no matter whether the actual sample is negative or positive, the result of the fluorescent PCR test will be negative. Displayed as positive, causing wrong guidance for disease diagnosis

Once the laboratory is contaminated by aerosols, it is difficult to clean up, the work is often forced to stop, and it takes a lot of time and money to clean up the pollution

Researchers have also used various methods to deal with aerosol pollution, such as ultraviolet irradiation, nuclease degradation, chemical substance oxidation, high temperature treatment, etc. Although these methods have certain effects, they are not ideal

For example, the invention patent (CN1493582A) proposes a method for degrading nucleic acids in organisms or microbial cells by using peroxides under ultraviolet light irradiation, metal ions or their simultaneous presence, but peroxides are unstable and degrade in a short time It becomes water and oxygen and loses its activity. In addition, the penetration of ultraviolet radiation is very weak, and it only acts on the surface of the object, and the treatment effect is not ideal.

Another example is the invention patent (CN1415381A) which proposes fumigation with a mixed solution of hydrochloric acid, nitric acid and acetic acid, and then uses an acid absorption device to absorb the acid in the room. This method also has a certain effect, but these acids are strong acids, which are corrosive to experimental equipment. Sex, and the process of absorbing acid is longer, and the effect is not ideal

Another example (CN110684605A) utilizes multiple ingredients to be mixed into a kind of nucleic acid removing agent, also obtains certain effect, but contains at least three kinds of ingredients and is a corrosive substance, and its mass fraction in the prescription is also higher, to The precision instruments in the laboratory are damaged to varying degrees, and the cost of purchasing such commercial products is relatively high for users

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