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Bacillus aerophilus, microbial agent and applications of bacillus aerophilus and microbial agent

A technology of Bacillus Aerophilus and microbial inoculum, applied in the biological field, can solve the problem of low microbial degradation rate and achieve the effect of improving efficiency

Inactive Publication Date: 2014-07-30
烟台大境生态环境科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among them, the biological treatment method has the advantages of good environmental compatibility, etc., but the efficiency of the biological treatment method is limited by the degradation rate of petroleum hydrocarbons by microorganisms, and the degradation rate of microorganisms that can degrade petroleum hydrocarbons is still low

Method used

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  • Bacillus aerophilus, microbial agent and applications of bacillus aerophilus and microbial agent
  • Bacillus aerophilus, microbial agent and applications of bacillus aerophilus and microbial agent

Examples

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preparation example Construction

[0020] Wherein, the preparation method of the microbial inoculum may include: inoculating Bacillus aerophilus in the culture medium and culturing, so that the total number of viable bacteria per gram of the microbial inoculum obtained is (2-300)×10 8 CFU. The temperature of cultivation can be 28-32°C.

[0021] Wherein, in order to further improve the effect of the microbial bacterial agent degrading petroleum hydrocarbons, preferably, the preparation method of the microbial bacterial agent may also include: mixing the material obtained after culturing in the fermentation medium with nutrient salts; Nutrients include NaBr, H 3 BO 3 , SrCl 2 , NaF, Potassium Sodium Tartrate and Sodium Malate. More preferably, the consumption of nutrient salt makes every kilogram of described microbial bacterial agent contain the NaBr of 50-100mg, the H of 10-40mg 3 BO 3 , 10-40mg of SrCl 2 , 1-5mg of NaF, 0.5-5g of potassium sodium tartrate and 10-100mg of sodium malate.

[0022] The pre...

Embodiment 1

[0027] This example is used to illustrate the cultivation of Bacillus aerophilus and the preparation of microbial agent of the present invention.

[0028] Bacillus aerophilus (Bacillus aerophilus) with preservation number CGMCC NO.8840 was inoculated into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L of NaCl and the rest of the water) Medium, cultured to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0029] Add 100mL of the above seed bacterial liquid into 100L fermentation medium (the fermentation medium contains 80g / L starch, 3g / L sodium chloride, 3g / L calcium carbonate, 5g / L potassium dihydrogen phosphate and 5g / L ferrous chloride), cultivated at 30°C, sampled during the cultivation and observed by microscope direct counting method, until the number of viable bacteria of Bacillus aerophilus per gram of culture solution was 10 9 CFU, the resulting culture solution is the microbial agent of this embodim...

Embodiment 2

[0031] This example is used to illustrate the cultivation of Bacillus aerophilus and the preparation of microbial agent of the present invention.

[0032] Bacillus aerophilus (Bacillus aerophilus) with preservation number CGMCC NO.8840 was inoculated into LB medium (containing 10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L of NaCl and the rest of the water) Medium, cultured to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0033] Add 100mL of the above seed bacterial liquid into 100L fermentation medium (the fermentation medium contains 80g / L starch, 3g / L sodium chloride, 3g / L calcium carbonate, 5g / L potassium dihydrogen phosphate and 5g / L ferrous chloride), cultivated at 30°C, sampled during the cultivation and observed by microscope direct counting method, until the number of viable bacteria of Bacillus aerophilus per gram of culture solution was 10 9 CFU, add 80mg of NaBr, 30mg of H 3 BO 3 , 20mg of SrCl 2 , 3mg of NaF, ...

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Abstract

The invention provides bacillus aerophilus with collection number of CGMCC No.8840. The invention also provides a microbial agent. The microbial agent comprises culture media and a thallus, wherein the thallus comprises bacillus aerophilus with collection number of CGMCC No.8840. The invention also provides applications of bacillus aerophilus and the microbial agent to degrading petroleum hydrocarbon. The marine petroleum pollution control efficiency can be more effectively improved by adopting bacillus aerophilus and the microbial agent.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a bacillus aerophilus, a microbial agent and applications thereof. Background technique [0002] With the development of offshore oil mining and transportation, the problem of marine oil pollution is becoming more and more serious. Drilling platform capsizes, oil pipeline bursts or oil tanker accidents often occur, such as the offshore pollution caused by the Dalian oil pipeline explosion in July 2010, and the Gulf of Mexico pollution caused by the collapse of the British Petroleum Company BP offshore drilling platform in April 2010, etc., according to the US National NOAA estimates that oil wells at the bottom of the Gulf of Mexico are leaking approximately 5,000 barrels of oil per day, and that as of June 1, 2010, 17 million to 27 million gallons of oil have leaked into the Gulf of Mexico. It will cause a large amount of oil to leak into the marine environment. If scientific and e...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34C12R1/07A62D101/20C02F101/32C02F103/08
Inventor 赵文勇阮志勇赵秉强王慧敏赵君才吕昭辉刘慧宋昭王彦伟刘小飞
Owner 烟台大境生态环境科技股份有限公司
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