31results about How to "Save experiment time" patented technology

The invention relates to a rock sample detection and data acquisition system. The system can realize elastic parameter test of a rock sample in the state of gas adsorption; in the system, a three-shaft pressure cabin is connected with a gas supply and metering control device for absorbing samples in the cabin and accurately metering absorption quantity, moreover, an ultrasonic transducer is installed on a cap of the (high) pressure cabin and integrated with an ultrasonic wave transmitting / receiving device so that the test of elastic parameters of methane absorption on coal can be realized truly. The system can automatically and continuously test in the processes of gas absorption and gas enrichment, thereby ensuring a consistent test environment, acquiring an absorption quantity and elastic parameter relation curve, improving test accuracy and efficiency and saving test time and cost. The rock sample detection and data acquisition system integrates methane enrichment mechanism simulation and rock physical property test, can be applied to seismic prospecting method research on coalbed methane and shale gas, mine advanced detection, coal mine safety research and the like.

The invention discloses a method for simultaneously compensating a scale factor and a zero offset of an optical fiber gyroscope by a one-time temperature experiment. The method comprises the following steps: (1) adjusting the temperature of an incubator, rotating the gyroscope clockwise and anticlockwise when the temperature reaches respective set temperature values, and acquiring temperature data acquired by a temperature sensor in the gyroscope in real time and data which correspond to the temperature and are output by the gyroscope in the whole process of switching on and switching off the incubator; (2) processing the temperature data and the data output by the gyroscope according to a simultaneous scale factor and zero offset compensation algorithm, and obtaining a temperature compensation coefficient matrix; (3) compensating the output of the gyroscope according to the temperature compensation coefficient matrix. According to the compensation method provided by the invention, the one-time temperature experiment is designed to simultaneously compensate the scale factor and the zero offset of the optical fiber gyroscope; a relation between a digital value output by the gyroscope and an input angle speed is directly expressed, so that the compensation coefficient matrix is obtained; therefore, the inaccuracy, which is caused by a zero offset error and the like, of compensation of the scale factor is avoided; furthermore, the temperature error of the gyroscope can be effectively compensated, and the method can save the experiment cost and is higher in practical significance.

The invention discloses a method for detecting vitamin A, D and E content in infant food and dairy products, comprising the following steps: accurately weighing a sample, adding a pyrogallic acid ethanol solution, adding a KOH aqueous solution for complete saponification, then rapidly cooling, and then placing the sample in a brown separating funnel, and irrigating an iodine flask by using an ethereal solution having a volume ratio of petroleum ether to ether being 2:1, putting the washing liquor in the brown separating funnel, shaking and standing until completely layering, carrying out extraction on the water layer by using another brown separating funnel filled with the ethereal solution, and combining the organic layers; washing the organic layers by using deionized water repeatedly until the organic layers are not alkaline, dewatering and filtering the organic layers, drying under nitrogen until nearly dried and then dissolving with methanol, blowing with a pressure blowing concentrator to 1 ml, bringing to volume by methanol, filtering through a filter membrane, and separating through a chromatographic column; separating by using 10% acetonitrile:methanol mobile phase with a flow rate of 0.3 mL / min; using three wavelengths of 296 nm, 264 nm, and 325 nm to simultaneously detect vitamin A, D and E, wherein, the detection concentrations respectively are 1.0-100.0 mu g / L, 1.0-100.0 mu g / L, and 0.50-50.0 mu g / L.

The invention relates to an experimental device used for testing minimum bending radius of an ocean flexible pipe cable. The experimental device is characterized by comprising two panels which are the same in shape and size and matched bolts, wherein the lower part of each panel is provided with horizontal bolt holes arranged into a horizontal line at intervals, the panels above the horizontal bolt holes are also provided with a plurality of groups of arc-shaped bolt holes, and each group of the arc-shaped bolt holes and the horizontal bolt holes can form configuration lines with different bending radiuses. The experimental device provided by the invention is simple to operate and convenient to manufacture and has the advantages of saving experimental time and reducing cost.

The invention provides a method and a device for measuring the continuous cooling phase transformation points of aluminum alloy. The method comprises the following steps of automatically collecting the data of resistance, temperature and time in real time by utilizing a computer, drawing a voltage-temperature curve or a resistance-temperature curve of an aluminum alloy sample according to the measured data, and then obtaining the cooling phase transformation points according to the drawn curve. The device comprises a heating insulation and cooling system, a dynamic voltage measuring system, a temperature measuring system, a signal amplifying and converting system and a data collecting and processing system which are sequentially connected. The device can be used for obtaining the curve of voltage changing along with the temperature in the continuous cooling process of a supersaturated solid solution, thereby judging the phase transformation points of the alloy. The invention has the advantages of wide cooling test range, high measuring precision, high speed and simple operation, and can carry out heating, insulation, cooling, and the voltage and temperature real-time measurement and collection on aluminum alloy materials.

An internal electron donor compound for preparing α-olefin polymerization catalyst component, including two kinds of electron donors; the proportion of the two kinds of electron donors in the compounding preparation of the catalyst is determined via designed experiments so as to obtain a catalyst component having good comprehensive performance or a particular performance. The electron donor compound of the present invention can be used in the preparation of α-olefin polymerization and co-polymerization catalyst component, particular the preparation of propylene polymerization catalyst component, and is applicable to prepare the propylene polymerization catalyst component by reacting magnesium chloride-ethanols complex compound carrier with titanium tetrachloride and electron donors, or to directly prepare the propylene polymerization catalyst component by reacting magnesium chloride, alcohols, titanium tetrachloride, and internal electron donor. In addition, also provided is a theoretical basis for selecting a proper electron donor combination from a plurality of electron donors.

The invention discloses a dynamic pressure regulating gas absorption and desorption experiment device based on a high-pressure volumetric method. The dynamic pressure regulating gas absorption and desorption experiment device comprises a dynamic pressure regulating inflation system, a vacuum degassing system, a gas absorption and desorption system, a constant-temperature system, a metering systemand a data processing system, wherein the dynamic pressure regulating inflation system is provided with a high-pressure gas cylinder, a pressure regulating valve, a high-pressure valve, a pressure gauge, a dynamic pressure regulating inflation tank and a three-way connector; the vacuum degassing system is provided with a vacuum pump, a motor, a vacuum diaphragm valve, a vacuum gauge tube, a vacuumgauge, a vacuum tank path, a high-pressure valve and a three-way connector; the gas absorption and desorption system is provided with an absorption and desorption tank, a pressure gauge and a high-pressure valve; the constant-temperature system is provided with a super constant-temperature water bath tank; the metering system is provided with a flow meter, a leveling bottle, a measuring cylinder,a glass piston, a sampling bag and a lifter; the data processing system is provided with a pressure sensor, a data collector, a computer and a printer. The dynamic pressure regulating gas absorptionand desorption experiment device disclosed by the invention has the advantages of convenience in operation, high experiment accuracy, accurate results and ability in achieving gas absorption and desorption experiments.

The invention provides a method for performing in-vitro accurate test on KRAS gene mutation, and the method is used for overcoming the defects that the conventional allele-specific polymerase chain reaction (PCR) method has insufficient specificity and false positive phenomenon. The method for performing the in-vitro accurate test on the KRAS gene mutation comprises the following steps of: (1) designing and synthetizing allele specific primers which comprise the allele specific primers espectively designed for 7 types of mutation of a typical KRAS gene exon 2 and are utilized as forward primers, wherein extension retardant primers are utilized as common downstream primers; (2) extracting the KRAS genomic deoxyribose nucleic acid (DNA) of a test sample, and additionally preparing the wild type genomic DNA (as wild type template); and (3) carrying out the real-time fluorescent PCR reaction. The test method has the advantages of strong specificity, high sensitivity, material conservation and time-saving property.

The invention discloses a diameter adjustable liquid bridge generator and belongs to the technical field of crystalline material preparation. The diameter adjustable liquid bridge generator comprises a support frame, wherein an upper liquid bridge support column mounting hole is formed in the upper part of the support frame and a lower liquid bridge support column mounting hole is formed in the lower part of the support frame; an upper liquid bridge support column is vertically arranged inside the upper liquid bridge support column mounting hole, a lower liquid bridge support column is vertically arranged inside the lower liquid bridge support column mounting hole, the upper liquid bridge support column corresponds to the lower liquid bridge support column, and a fastening bolt of the upper liquid bridge support column is arranged on the support frame located on the side of the upper liquid bridge support column mounting hole; an upper liquid bridge support column cap is fixedly arranged on the upper end of an upper liquid bridge support column king rod, and an upper liquid bridge support column upper case is arranged at lower end of the upper liquid bridge support column king rod through a screw; a lower liquid bridge support column lower bar is fixedly connected with the lower end of a lower liquid bridge support column king rod through a lower liquid bridge support column snap ring; and the upper end of the lower liquid bridge support column lower bar is connected with the lower liquid bridge support column lower case through the screw.

The invention relates to a high time resolution three-dimensional imaging method based on a framing camera. The high time resolution three-dimensional imaging method based on the framing camera includes the following steps: 1), obtaining a two-dimensional image of an object to be tested by utilization of the framing camera; and 2), performing three-dimensional reconstruction on the obtained two-dimensional image of the object to be tested to obtain the three-dimensional visual effect object to be tested. The high time resolution three-dimensional imaging method based on the framing camera is capable of obtaining the three-dimensional image with picosecond (10-12 S) time resolution, and providing a reliable basis for direct improving on laser beam forms in a test process.

The invention discloses a near-field electromagnetic wave measuring system. The near-field electromagnetic wave measuring system comprises a two-dimensional plane moving system, a principal computer,a vector network analyzer, a lifting table, a horizontal adjusting apparatus and a near-field measuring probe; the principal computer is connected with the vector network analyzer, the input end of the vector network analyzer is connected with the near-field measuring probe, the output end is connected with a sample, and the sample is arranged on the horizontal adjusting apparatus; the horizontaladjusting apparatus is fixed on the lifting table; the lifting table is fixed on the two-dimensional plane coordinate moving system; and the near-field measuring probe is fixed in a space above the sample. The near-field electromagnetic wave measuring system not only can be used for measuring a waveguide (resonant cavity, photo crystal, negative refraction materials and the like) sample, but alsocan be used for measuring an antenna sample in an open environment, can be implemented by conveniently adjusting partial parts, can effectively solve the problem of excessively high experimental cost,can also be used for measuring various samples, and can also effectively save the experimental time and space.

The invention discloses specific primer probe combinations for detecting HLA-A*24:02 allelic genes and a kit and a detection method. Specifically, on the basis of a TaqMan probe detection method, twopairs of primer probe combinations for highly specifically amplifying HLA-A*24:02 allelic genes are designed, namely an upstream primer Fp1: 5'-GGTTCTC ACACCCTCCA GATGATGGT-3', a downstream primer Rp1: 5'-CTCCAGGTATCTGCGGAGCACG-3', a probe 1: 5'-FAM-CCACTTGCGCTTGGTGATCTGAGCC-BHQ2-3', an upstream primer Fp2: 5'-CTCGTCCC CAG GCTCCCTC-3', a downstream primer Rp2: 5'-CACCGGCCTCGCTCTGGT-3', a probe 2:5'-CY5-GAAGGCCCACTCACAGACTGACCGAG-BHQ2-3'; FAM is 6-carboxyfluorescein; Cy5 is Cyanine 5; BHQ2 is Black Hole Quencher-2; the two specific primers and probes together with reference gene primers and probes are put into a same tube to implement a multiple fluorescent PCR (Polymerase Chain Reaction), and results are analyzed through fluorescent amplification curves. The specific primer probe combinations have the characteristics of being good in specificity, flexible and rapid, high in flux, free of pollution, high resolution rate, possible in real-time reaction process monitoring, and the like,and are applicable to detection on whole genome DNA (Deoxyribonucleic Acid) samples HLA-A*24:02 in human peripheral blood and saliva.

The invention relates to a solid-phase extraction device. The solid-phase extraction device comprises a sampling pump, a pressure meter, a flow meter and a vertically arranged solid-phase extraction unit which are connected with one another in series, wherein the solid-phase extraction unit comprises a lower external adapter, an upper external adapter, a connector and two filtration tanks; the top of the lower external adapter is fixedly connected with the bottom of a first filtration tank; the top of the first filtration tank is detachably connected with the bottom of the connector; the top of the connector is detachably connected with the bottom of a second filtration tank; the top of the second filtration tank is fixedly connected with the bottom of the upper external adapter; the bottom of the lower external adapter and the top of the upper external adapter penetrate through a flow guide pipe; the first filtration tank, the second filtration tank and the connector are internally connected with one another; annular limiting slots are formed in the periphery of the inner wall of the connector; a non-return pressing sheet, a solid-phase extraction film and a support screen plate are arranged in sequence from top to bottom of each limiting slot. The solid-phase extraction device is easy to operate and convenient to transport, and is capable of preprocessing water samples on sampling sites.

The invention discloses an EBSD sample preparation method for cemented carbide powder. The EBSD sample preparation method comprises the following steps: mixing and batching of powdery samples; compression molding; sintering; inlaying; grinding and polishing; and sample cleaning. According to the invention, raw cemented carbide powder and Cu powder are fully and uniformly mixed, and the obtained mixture is sintered in a thermal analyzer with precise temperature control and a protective atmosphere; the raw cemented carbide powder is cured in virtue of a Cu phase, and the aggregates of a WC raw material are perfectly dispersed; the optimal parameters of EBSD grinding, polishing and sample preparation are obtained through optimization, so good EBSD testing results can be obtained; and application of large equipment is omitted, so pressing and sintering cost is greatly reduced and an experimental period is shortened.

The invention relates to a method of testing the elasticity of a PTT / PET (polytrimethylene terephthalate / polyethylene glycol terephthalate) self-crimping filament by using hank knotting thereof. The method is characterized by comprising the following steps: step 1, making self-crimping filament hank knotting; step2, performing dry heat treatment to the hank knotting; step3, carrying out hank knotting tensile testing; step 4, calculating the elastic elongation ratio. The method has the following beneficial effects: (1) the elastic property of the self-crimping filament can be accurately predicted in fabric at a fiber stage, thus the experimental cost and time are greatly reduced in contrast with a current method of testing the elasticity after trial-producing the fabric; (2) the operation is simple, and the test method is conveniently applied to industrial production; (3) the representativeness of the elastic elongation ratio of the hank knotting is greatly improved in contrast to that of a monofilament, thus the experimental times are reduced, and the experimental precision is improved.

The invention discloses a test worktable with a test tube cleaning function. The test worktable comprises a right base comprising a test worktable top, and a cleaning cavity is formed in the base; a water inlet and a water outlet are formed in the upper and lower ends of the left side of the cleaning cavity separately, and an inside cleaning assembly is arranged in the cleaning cavity; a transmission cavity is arranged at the upper end of the left side of the cleaning cavity, and a transmission assembly is arranged in the transmission cavity; an outside cleaning assembly used for cleaning theouter wall of a test tube is arranged on the right side of the transmission cavity; and the lower end wall of the transmission cavity is rotatably provided with a lifting screw rod which extends intothe cleaning cavity, and the lower side of the lifting screw rod comprises a stop block. The used test tube is arranged on a hollow support rod in a backoff mode; after the upper side of the test tubeis clamped, the test tube is driven by a lifting platform to slide into the cleaning cavity downwards; water is sprayed to the outer side of the test tube through nozzles for cleaning the outer wallof the test tube while the test tube is slide; and the inner wall of the test tube is cleaned during rotation of the hollow support rod, and the test tube does not need to be cleaned by experiment personnel.

The invention discloses a xenopus laevis oocyte expression carrier with a yellow or red fluorescence protein label and application. pGH19 plasmid is modified through a molecular biology means, and isspecifically developed to be a carrier pGH19-EYFP or pGH19-mRFP which is suitable for expression of the xenopus laevis oocyte and contains the enhanced yellow or monomer red fluorescence protein label. The carrier can normally express the target gene, and can detect the distribution situation and the expression mode of the target protein with the yellow or red fluorescence label through a fluorescence microscope or a confocal microscope, and thus a more visual and simpler detection method can be provided for the protein situation of xenopus laevis oocyte expression. The spatiotemporal dynamicsof the protein expression can be observed in real time. The expression situation of the target protein can be detected only by using an universal antibody as for the following extraction, purification, identification and functional analysis of the target protein, the experimental process is greatly simplified, and the experimental time is saved and economic cost is reduced.

The invention belongs to the technical field of soil microbial community diversity analysis and discloses optimal conditions and reagent dosage in a PCR-DGGE (Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis) method used for analyzing microbial community diversity of soil polluted by petroleum. The analysis method comprises the following steps of: 1, extracting enough DNA from 0.4g of soil samples; 2, carrying out PCR (Polymerase Chain Reaction) on 1 microliter of purified DNA; 3, performing a DGGE (denaturing gradient gel electrophoresis) experiment on 3-5 microliters of PCR products; 4, adding 9 microliter of TEMED (tetramethylethylenediamine) and 40 microliter of 10% APS (ammonium sulfate) into 18ml of modified acrylamide glue solution for preparing gel; 5, filling the gel, covering the top with a preservative film, standing still overnight to finally obtain excellent modified gel; 6, performing electrophoresis for 5 hours at the voltage of 120V and the electrophoresis temperature of 60 DEG C to obtain a good DGGE map; and 7, performing silver staining, wherein a stop solution is not used necessarily. The method is mainly used in the molecular ecology researches in the bioremediation process of soil polluted by petroleum, and also can provide references for molecular ecology researches of other environmental samples.

The invention discloses a primary isolated culture and induced differentiation method for precursor fat cells in adult yak muscles. The method comprises the steps of disinfection treatment and segmentation, digestion, screening and filtration, centrifugation, purification and culture. According to the cell culture for the precursor fat cells in the adult yak muscles, provided by the invention, manpower and material resources can be saved, and the limitation of high pollution rate caused by using newborn calf fat tissues and primary cells in a traditional culture method is broken through, so that the source approach of samples is wider, and a large number of cells can be obtained at the same time; and the culture method is a convenient and efficient precursor fat cell culture method, provides a molecular basis for subsequent in-depth research on gene and protein expression, signal channels and the like in proliferation and differentiation process of intramuscular precursor fat cells, provides a new thought and a new method for research on fat deposition, fat metabolism, fat mobilization and the like of different parts, and has an important significance in improving the meat quality of yaks.

The invention discloses a volume and weight measuring device and method for vegetables and fruits in irregular shapes. The device comprises a base, a water storage tank arranged on the base, a weighing sensor arranged at the upper end of the water storage tank and a sample box arranged at the upper end of the weighing sensor, and a sample box water inlet pipe is arranged at the top end of the sample box; a sample box downcomer on the side surface of the lower end of the sample box is provided with a sample box downcomer manual valve; the top of the water storage tank is provided with a first funnel-shaped water receiving port which is opposite to the outlet of the sample box downcomer; a water pump is arranged at the bottom of the water storage tank; the outlet of a water feeding pipe connected with the water pump is opposite to a second funnel-shaped water receiving opening in the top end of the water inlet pipe of the sample box, a funnel-shaped water inlet is formed in the top end of the water storage tank, a liquid level meter is arranged on the side face of the water storage tank, an electric control box with a rechargeable battery is arranged on the side face of the water storage tank, and a sample box liquid level sensor is arranged in the sample box. The volume and weight measuring device and method are suitable for field edges, are convenient to move, and can accurately measure the volume and the weight of vegetables and fruits with irregular shapes.

The invention provides a method for calculating and predicting thermoelectric properties of a material based on a high-flux first principle. The method comprises the following steps of: 1, carrying out the primary magnetic test calculation of an initial crystal structure of a material, so as to obtain the magnetic moment information of the material; 2, carrying out structure optimization calculation on the compound by using a conjugate gradient algorithm or a quasi-Newton algorithm to obtain an optimized unit cell structure, then carrying out calculation and fitting on the bulk modulus of the material to obtain bulk modulus information of the corresponding material, and further deriving sound velocity information of the material; 3, after structural optimization calculation convergence, carrying out self-consistent calculation of the material to obtain the charge density, the total energy and the magnetic moment of the material; and 4, calculating the thermoelectric property based on the charge density calculated in the step 3.

The invention discloses a method for conveniently detecting the dispersity of cobalt salt in a rubber material. The method comprises the following steps: (1) polishing a sample to be detected until the distance between a steel wire cord and the surface of the rubber material is 0.05-0.5 mm; and (2) stripping the steel wire cord along the polished surface of the rubber material through tension detection equipment, observing the surface state of the stripped steel wire cord and a tension value displayed by the tension detection equipment, if the tension value is greater than a preset value and the dew point of no rubber on the surface of the steel wire cord is less than 2, indicating that the adhesive force of the rubber material is qualified, otherwise, indicating that the adhesive force of the rubber material is unqualified. According to the method, the steel wire cord is stripped from the surface of the rubber material, the expression mode is consistent with that of stripping of the rubber material and the steel wire cord in the tire U-shaped explosion, and the rubber material problem can be well represented.

The invention discloses an electroplating device of an electrode for electrophysiological experiments. An electroplating reactor contains at least an electroplating operating room which is composed of a sealed U-shaped tube. A silver filament to be electroplated passes through a central hole of a metal thin tube at one end of the U-shaped tube and then is immersed into an electroplate liquid to form an electroplating anode electrode. The other end of the U-shaped tube is provided with an electroplating cathode electrode and an opening and closing cover opening. A power supply controls the electroplating process through a control module which is composed of a circuit for keeping current constant and a circuit with a timing function. The electroplating operating room is also equipped with an electroplate-liquid level detector for liquid level of the electroplate liquid. By the adoption of a control circuit, current is controlled to be stable and electroplating time is controlled to be constant; it is ensured that thickness of silver chloride covering the surface of the silver filament electroplated each time is uniform and unified; and uncontrollable factors in an experiment are minimized. In addition, multiple electroplating tanks are also externally connected. Thus, high-flux electroplating is realized; multiple silver filaments can be electroplated at a time; and electroplating is more convenient, rapid and efficient.

The LNG tank inner and outer layer spacing experiment device comprises a low-temperature chamber and a normal-temperature chamber, the low-temperature chamber comprises a low-temperature chamber shell, an experiment opening is formed in the top of the low-temperature chamber shell, a low-temperature chamber body heat insulation layer is arranged on the inner wall of the low-temperature chamber shell, and an experiment position heat insulation layer is arranged on the edge of the experiment opening; an LNG tank body inner cylinder simulation plate is arranged at the experiment opening, cold insulation foamed plastic is arranged on the upper surface of the LNG tank body inner cylinder simulation plate, and the outer edge of the LNG tank body inner cylinder simulation plate is connected with the top of the low-temperature chamber shell; a limiting frame is arranged at the top of the low-temperature chamber shell, the LNG tank body inner barrel simulation plate is located in the limiting frame, an outer ring heat insulation layer is arranged on the inner wall of the limiting frame, an LNG tank body outer barrel simulation plate is movably arranged above the LNG tank body inner barrel simulation plate, and the experiment space is used for being filled with an experiment heat insulation layer. The LNG tank body cold insulation interval experiment device is used for carrying out an experiment on the interval between the LNG tank body inner cylinder and the LNG tank body outer cylinder, is used for selecting the LNG tank body with a proper cold insulation interval, and is beneficial to reducing the size and materials of the LNG tank body.

The invention discloses a detection device for detecting the durability of a vehicle shock absorber sleeve assembly, and aims to solve the problems that the actual use condition of the sleeve assembly cannot be effectively simulated, the test time is long, and the design and development progress of a whole vehicle and a shock absorber is delayed in the prior art. The device comprises a support, a mounting plate, a first actuator, a second actuator, three bearings and a torsion bar, the mounting plate is connected with the support, the three bearings are all connected with bearing seats, the three bearing seats are all connected with the mounting plate, the torsion bar penetrates through the three bearings, a third shaft sleeve is in clearance fit with a third bearing, and the third shaft sleeve is in clearance fit with the third bearing. A torsion arm is arranged at the end, close to the first bearing, of the torsion bar, the torsion arm is connected with a second connecting piece and a second actuator, a to-be-tested part is installed at the position, between the second bearing and the third shaft sleeve, of the torsion bar, and a first connecting piece is installed on the to-be-tested part and connected with the first actuator.

The invention discloses a method for detecting the receptor-ligand binding blocking capacity of a compound by using an HTRF technology, and belongs to the field of biochemical detection. The method for detecting the receptor-ligand binding capacity by utilizing the HTRF technology comprises the following steps of: 1, carrying out fluorescent donor labeling on a ligand; 2, carrying out fluorescent receptor labeling on a receptor; 3, co-incubating the receptor, the ligand and a tested compound; and 4, detecting a fluorescence signal of the receptor, and calculating the receptor-ligand binding blocking capability of the compound. The method has the advantages of homogeneous reaction, simple operation and high throughput test.

The invention provides a pre-coating Polio virus I, II and III D antigen detection method for batch production. Bovine (rabbit and sheep) anti-I, II and III Polio virus D antigens are employed to purify an antibody-coated microporous plate, also a protective agent is employed for protection of a coated plate and coating antibodies so as to prepare a pre-coated plate, and the pre-coated plate is matched with horseradish peroxidase labeled bovine (rabbit and sheep) anti-I, II and III Polio virus enzyme-labeled antibodies and other reagents at the same time. The method (reagent) can achieve specific qualitative and quantitative detection of Polio virus I, II and III D antigens, has no cross with Polio virus I, II and III C antigens and other enteroviruses, and is a type I, II and III Polio virus D antigen combined detection method with the advantages of high specificity, high sensitivity, convenient use and extensive use, and has high application value in production and verification of IPV vaccines.

The invention discloses a method of detecting multiple cell factors simultaneously by HTFR (homogeneous time-resolved fluorescence) technique and belongs to the field of detection of cell factors. Themethod herein comprises the steps of I, setting cell density with a cell culture board, and culturing cells with a culture system; II, treating the cells with a drug, and acquiring a strongest signalby optimizing the stimulation time; III, collecting cell supernate, and configuring standard curves if the cell supernate requires diluting; IV, adding 16 ul of the cell supernate into a detection board, adding 4 ul of a marked detection antibody mixture, and incubating at room temperature; V, acquiring a reading with a microplate reader, and calculating concentrations of the cell factors in a sample respectively through their respective standard curves. The method herein has the advantage that the novel method is suitable for detecting multiple factors just through reagents.

The invention discloses a method for screening a combined blocking agent among Bcl-2 family members by utilizing an HTRF one-step method, and belongs to the field of blocking agent screening methods.The method for screening the blocking agent combined between Bcl-2 family members (such as Mcl1 / BAK) by utilizing the HTRF one-step method comprises the following steps: configuring constructed Mcl1and BAK proteins with different labels, and screening a sample; sequentially adding the Mcl1 and the BAK proteins of the sample to be screened into a microporous plate, wherein the two corresponding anti-label antibodies are respectively coupled with Donor and Acceptor; incubating at room temperature, and detecting fluorescence by using a microplate reader, wherein the ratio of 665nm / 620nm is original data; according to the method, screening the combined blocking agent among Bcl-2 family members (such as Mcl1 / BAK) at the biochemical level can be achieved, and constructing cells is not needed,and the method is more direct and quicker.

A precision testing device for a current-carrying friction pair of a conductive slip ring of a satellite disclosed by the present invention comprises at least one group of testing assemblies, each group of testing assemblies comprises an upper sample loading measurement mechanism and a lower sample detachable positioning mechanism, and the lower sample detachable positioning mechanism comprises a lower sample positioning support block and a lower sample positioning fixing block. The lower sample positioning fixing block and the lower sample positioning supporting block are circumferentially positioned through a lower sample positioning structure; a lower sample reciprocating swing driving mechanism drives the lower sample to swing back and forth in the circumferential direction; and the upper sample loading measurement mechanism comprises an upper sample clamp, an upper sample positioning and supporting block, an upper sample positioning and fixing block and a shear stress sensor group, an upper sample is driven to move through an upper sample displacement loading module, and the magnitude of force borne by the upper sample is detected through the shear stress sensor group. The device disclosed by the invention has the advantages that the position consistency of the sample after being disassembled and reassembled is ensured, and the circumferential reciprocating swing of the lower sample can be realized.