Method for detecting vitamin A, D and E content in infant food and dairy products

Abstract

The invention discloses a method for detecting vitamin A, D and E content in infant food and dairy products, comprising the following steps: accurately weighing a sample, adding a pyrogallic acid ethanol solution, adding a KOH aqueous solution for complete saponification, then rapidly cooling, and then placing the sample in a brown separating funnel, and irrigating an iodine flask by using an ethereal solution having a volume ratio of petroleum ether to ether being 2:1, putting the washing liquor in the brown separating funnel, shaking and standing until completely layering, carrying out extraction on the water layer by using another brown separating funnel filled with the ethereal solution, and combining the organic layers; washing the organic layers by using deionized water repeatedly until the organic layers are not alkaline, dewatering and filtering the organic layers, drying under nitrogen until nearly dried and then dissolving with methanol, blowing with a pressure blowing concentrator to 1 ml, bringing to volume by methanol, filtering through a filter membrane, and separating through a chromatographic column; separating by using 10% acetonitrile:methanol mobile phase with a flow rate of 0.3 mL/min; using three wavelengths of 296 nm, 264 nm, and 325 nm to simultaneously detect vitamin A, D and E, wherein, the detection concentrations respectively are 1.0-100.0 mu g/L, 1.0-100.0 mu g/L, and 0.50-50.0 mu g/L.

Description

technical field [0001] The invention belongs to a method for determining the content of vitamin ADE in food for infants and young children and dairy products. Background technique [0002] Vitamin A (retinoL), vitamin D (VitaminD 2 and Vitamin D 3 ) and vitamin E (α-E, γ-E, and δ-E) are fat-soluble vitamins necessary for the body to maintain normal metabolism and function [1]. Vitamin A, also known as retinol, has the functions of promoting body growth, maintaining epidermal integrity, reproduction, bone and vision; vitamin D mainly includes vitamin D 2 (ergocalciferol) and D 3 (cholecalciferol), they can promote calcium and phosphorus metabolism and bone formation in mammals; vitamin E is also called tocopherol, mainly including α-E, γ-E and δ-E, among which α-tocopherol is biological It has the highest effect and has the functions of promoting fertility and anti-aging. Since these 6 kinds of vitamins are widely used in the production and processing of the food industr...

AI Technical Summary

Problems solved by technology

The national health standard method cannot simultaneously determine these 6 vitamins, and the measurement time is long and the method is cumbersome, which may cause the loss of fat-soluble vitamins in the sample and reduce the recovery rate

It not only consumes a lot of testing time and manpower, but also adds many influencing factors to the stability of the experimental results, which is not conducive to the systematic analysis of the test results, and is also a waste of limited resources.

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