Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting receptor-ligand binding blocking capacity of compound by using HTRF technology

A technology of technical detection and combination ability, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of difficult popularization and high price, and achieve the effects of stable signal, saving experimental time, and long-lasting fluorescence

Pending Publication Date: 2021-11-09
SUZHOU GENHOUSE PHARMA RES & DEV CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] To sum up, the existing detection methods all require special instruments and are expensive, and some even require custom-made consumables, such as isotopic markers, which are difficult to popularize

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting receptor-ligand binding blocking capacity of compound by using HTRF technology
  • Method for detecting receptor-ligand binding blocking capacity of compound by using HTRF technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1, the androgen receptor containing the GST tag is labeled with the GST antibody of the chimeric lanthanide Tb;

[0040] Step 2, labeling androgen with FITC;

[0041] Step 3, incubating the serially diluted Darolutamide together with the labeled androgen receptor and androgen at room temperature;

[0042] Step 4, detecting the receptor fluorescence signal, and calculating the ability of the compound to block receptor-ligand binding;

[0043] Such as figure 2 As shown, Darolutamide dose-dependently blocks androgen receptor and androgen binding.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting the receptor-ligand binding blocking capacity of a compound by using an HTRF technology, and belongs to the field of biochemical detection. The method for detecting the receptor-ligand binding capacity by utilizing the HTRF technology comprises the following steps of: 1, carrying out fluorescent donor labeling on a ligand; 2, carrying out fluorescent receptor labeling on a receptor; 3, co-incubating the receptor, the ligand and a tested compound; and 4, detecting a fluorescence signal of the receptor, and calculating the receptor-ligand binding blocking capability of the compound. The method has the advantages of homogeneous reaction, simple operation and high throughput test.

Description

technical field [0001] The invention belongs to the field of high-throughput compound screening, and specifically relates to a method for detecting compound blocking receptor-ligand binding ability by using HTRF technology. Background technique [0002] Receptor is a cellular protein component formed during the evolution of cells, which can recognize a certain trace chemical substance in the surrounding environment, first bind to it, and trigger subsequent physiological responses through the intermediary information transduction and amplification system or pharmacological effects. [0003] Since Langley put forward the receptor theory 100 years ago, receptors have been proved to be objectively existing entities with various types and mechanisms of action have been elucidated. Now receptors are no longer an empty and general concept. [0004] The content of receptor molecules in cells is very small, and 1 mg of tissue generally only contains about 10 fmol. A substance that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/58G01N33/53
CPCG01N21/6408G01N33/582G01N33/53
Inventor 张涛李家鹏王奎锋
Owner SUZHOU GENHOUSE PHARMA RES & DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com