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Application of high-content dirhamnolipid of pseudomonas aeruginosa in bio-remediation

A technology of Pseudomonas aeruginosa and double rhamnolipid is applied in the application field of high-content double rhamnolipid in bioremediation, and can solve the problems of inability to be degraded by microorganisms, secondary pollution, inhibition, etc. Improves bioavailability, promotes dissolution, and shortens time

Inactive Publication Date: 2012-02-01
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Traditional chemical surfactants have great disadvantages: because they cannot be degraded by microorganisms, they usually cause secondary pollution, and may inhibit the activity of degrading bacteria in bioremediation

Method used

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  • Application of high-content dirhamnolipid of pseudomonas aeruginosa in bio-remediation
  • Application of high-content dirhamnolipid of pseudomonas aeruginosa in bio-remediation
  • Application of high-content dirhamnolipid of pseudomonas aeruginosa in bio-remediation

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Embodiment 1

[0020] Pseudomanas aeruginosa W 3 Isolated from the long-term petroleum-polluted soil near Wuhan Petrochemical, the strains were transferred and stored on LB slant medium, the medium composition: 10g / L NaCl, 10g / L peptone, 5g / L yeast powder, add water to 1000mL, pH 7.2, among them, Pseudomonas aeruginosa, which is Pseudomonas aeruginosa W 3 , the deposit number is CCTCC NO: M2010048, and the deposit date is March 09, 2010.

[0021] The composition of the seed medium is: 10g / L NaCl, 10g / L peptone, 5g / L yeast powder, add water to 1000mL, 15g / L yeast powder, pH 7.2. The amount of liquid in the Erlenmeyer flask is 20%, the culture temperature is 30-37 DEG C, the rotating speed of the shaker is 150-180rpm, and after 24-36 hours of culture, the inoculation amount of 5-10% is inoculated into the fermentation medium.

[0022] Fermentation medium composition: 0.7g / L KH 2 PO 4 , 0.9g / L Na 2 HPO 4 , 2g / L NaNO 3 , 0.4g / LMgSO 4 ·7H 2 O, 0.1g / L CaCl 2 2H 2 O, 20g / L mannitol; trac...

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Abstract

The invention relates to an application of high-content dirhamnolipid of pseudomonas aeruginosa in the bio-remediation. The high-content dirhamnolipid produced by the fermentation of pseudomonas aeruginosa is mainly applied in the bio-remediation field of polluted soil or water body. The invention has the following advantages: the content of dirhamnolipid in the dirhamnolipid mixture is as high as 90%; by the use of the high-content dirhamnolipid, water surface tension can be reduced from 71.4 mN / m to 30.5 mN / m; the critical micelle concentration is 48mg / L; the high-content dirhamnolipid has high surface activity; under extreme conditions of high temperature, high salt and high pH and the like, the high-content dirhamnolipid can still maintain high surface activity and emulsifying capacity, can remarkably promote biodegradation of polycyclic aromatic hydrocarbons and has a latent application value in the bio-remediation.

Description

【Technical field】 [0001] The invention relates to the technical field of surfactants, in particular to the application of a high-content dirhamnolipid of Pseudomonas aeruginosa in bioremediation. 【Background technique】 [0002] Biosurfactant is a biomolecule produced by microorganisms, which can significantly reduce surface and interfacial tension, has low toxicity to the ecological environment, and can eventually be degraded by microorganisms, so that biosurfactants can gradually replace chemically synthesized ones in many aspects. Surfactants are used more and more widely. [0003] Rhamnolipid belongs to a kind of glycolipid surfactant, which is an extracellular metabolite produced by microorganisms growing to a certain stage in the fermentation process under suitable conditions. It is a kind of biosurfactant with better effect. Like other synthetic surfactants, its molecular structure is hydrophilic and hydrophobic, and has multiple functions such as solubilization, emul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/02C02F3/34B09C1/10C12R1/385
Inventor 崔长征沈萍张甲耀
Owner EAST CHINA UNIV OF SCI & TECH
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