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Pseudomonas aeruginosa, fungicide and application of pseudomonas aeruginosa and fungicide to degradation of petroleum and/or petroleum products

A technology of Pseudomonas aeruginosa and petroleum products, applied in the direction of application, plant products, bacteria, etc., to achieve the effect of large biomass, high tolerance, and fast growth speed

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil pollution in oil fields usually coexists with oil pollution and salt pollution caused by oilfield produced water, which is the biggest challenge for bioremediation

Method used

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  • Pseudomonas aeruginosa, fungicide and application of pseudomonas aeruginosa and fungicide to degradation of petroleum and/or petroleum products
  • Pseudomonas aeruginosa, fungicide and application of pseudomonas aeruginosa and fungicide to degradation of petroleum and/or petroleum products
  • Pseudomonas aeruginosa, fungicide and application of pseudomonas aeruginosa and fungicide to degradation of petroleum and/or petroleum products

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Experimental program
Comparison scheme
Effect test

Embodiment approach

[0052] According to a specific embodiment of the present invention, the inorganic salt is KH 2 PO 4 , K 2 HPO 4 , NH 4 NO 3 , MgSO 4 , CaCl 2 and FeSO 4 ·7H 2 O.

[0053] Wherein, the present invention has no special limitation on the amount of the inorganic salt added, which can be determined according to the type and content of the inorganic salt in the environment polluted by petroleum and / or petroleum products. For example, in KH 2 PO 4 (0.8-1.2%), K 2 HPO 4 (0.8-1.2%), NH 4 NO 3 (1-1.5%), MgSO 4 (0.03-0.08%), CaCl 2 (0.001-0.003%), FeSO 4 ·7H 2 O (0.01-0.03%) mixed inorganic salt solution is an example, based on per kilogram of the environment containing petroleum and / or petroleum product pollution, the addition amount of the above-mentioned inorganic salt mixed solution can be 1-3ml / day.

[0054] According to the present invention, when the environment containing petroleum and / or petroleum products is soil, in order to further promote the degradation e...

Embodiment 1

[0066] This example is used to illustrate the salt-tolerant characteristics of bacterial strain P9 provided by the present invention in petroleum-containing environment

[0067] In the triangular flask, add 50g of the original oil content of 1.5% by weight of the petroleum-contaminated soil sample, 200mL inorganic salt culture medium and some glass beads, in addition, add sodium chloride in the glass bottle at the same time, so that the concentration of sodium chloride is respectively 0, 3% by weight, 5% by weight, 7.5% by weight, 9% by weight, each salt concentration was repeated three times, and sterilized by moist heat at 121° C. for 15 minutes. It has been determined that 5% of petroleum hydrocarbons will be lost during the sterilization process.

[0068] The bacterial strain P9 of the present invention was inoculated in the Erlenmeyer flasks containing different sodium chloride concentrations above, and the inoculum size was 1.25 mL. Place each Erlenmeyer flask in a 30°C...

Embodiment 2

[0079] This example is used to illustrate the impact of different N forms on the degradation effect

[0080] Add petroleum substrate (concentration: 2000mg / L) and different N forms (NH 4 ) 2 SO 4 , (NH 2 ) 2 CO 3 、KNO 3 , NH 4 NO 3 Inorganic salt culture solution (the equimolar amount of nitrogen-containing inorganic salt in the original inorganic salt medium is replaced by the above-mentioned nitrogen-containing inorganic salt), adjust pH=9, inoculate 3% bacterial solution in the above-mentioned Erlenmeyer flask, at 30 ℃, 150r / min shaking culture for 7 days, then measure the TPH content, and calculate the removal rate; at the same time, a blank control experiment without bacterial solution was set up for each different N form treatment.

[0081] The results showed that adding (NH 2 ) 2 When CO nitrogen source is used, the degradation rate reaches 71%. Add NH 4 NO 3 When nitrogen source is used, the degradation rate reaches 60%. Add KNO 3 When nitrogen source is...

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Abstract

The invention relates to the field of microbial remediation environments, particularly discloses a pseudomonas aeruginosa and a fungicide with the pseudomonas aeruginosa, wherein the preservation number of the pseudomonas aeruginosa is CGMCC No.10849. The pseudomonas aeruginosa can survive and grow in the environments of a sole carbon source containing petroleum and / or petroleum products when inorganic salt culture media are provided, is high in life force, rapid to grow and large in biomass and has high tolerance and degrading capability for the petroleum and / or petroleum products. The pseudomonas aeruginosa can tolerate high-salinity environments and can effectively degrade petroleum and / or petroleum products in the environments.

Description

technical field [0001] The present invention relates to the field of microbial environment restoration, in particular to a strain of Pseudomonas aeruginosa, bacteria agents containing the Pseudomonas aeruginosa, their application in degrading petroleum and / or petroleum products and a method for degrading petroleum and / or methods of petroleum products. Background technique [0002] Oil pollution is one of the important causes of farmland pollution. With the rapid development of my country's economy and the increasing amount of oil extraction and use, a large amount of oil and its processed products have entered the environment, which has inevitably polluted the environment. [0003] About 70,000 tons of ground crude oil enters the soil environment every year in my country's oil companies, causing serious damage to the ecological environment. After petroleum pollutants enter the soil, they will destroy the soil structure and affect the permeability of the soil, hinder the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12N1/21C12N1/15A01H5/00C12R1/385
CPCY02A40/135
Inventor 林森朗喆任立伟王培红
Owner 粮华生物科技(北京)有限公司
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