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Bacillus cereus phage lyase, preparation method and application thereof

A phage lyase, Bacillus cereus technology, applied in the field of bioengineering, can solve problems such as increased antibiotic resistance

Active Publication Date: 2018-09-14
HAINAN NORMAL UNIV +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

With the extensive use of antibiotics, the resistance of pathogenic bacteria to antibiotics has gradually increased, and even a large number of multidrug-resistant bacteria have emerged (Simm R, Voros A, Ekman JV et al : BC4707 Is a majorfacilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance. Plos One 2012, 7(5))

Method used

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  • Bacillus cereus phage lyase, preparation method and application thereof
  • Bacillus cereus phage lyase, preparation method and application thereof
  • Bacillus cereus phage lyase, preparation method and application thereof

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Embodiment Construction

[0021] In order to facilitate a further understanding of the present invention, the examples provided below illustrate it in more detail. However, these examples are only for a better understanding of the invention and are not used to limit the scope or implementation principle of the invention, and the embodiments of the invention are not limited to the following content.

[0022] Preparation of Lyase PlyHSE3

[0023] Using the DNA of Bacillus cereus phage vB_BceM-HSE3 (CCTCC M 2017798) as a template, the primer pair Gp100-For / BamH was designed (5’-GCGGATCCATGGGTTTCAAAAATGAA-3’) and Gp100-Rev / Sal (5'-GAGTCGACTTAGTTAGATACGAATC-3') for gene gp 100 amplification. will amplify the gene gp 100 and the vector pET28a respectively using restriction enzymes Bam H I and Sal I was digested and connected with T4 ligase to construct the recombinant plasmid pET28a- gp 100, and then transformed into Escherichia coli BL-21 (F − dcm ompT hsdS B (r B − m B − ) gal (...

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Abstract

The invention relates to the bioengineering field, in particular to application of a phage lyase PlyHSE3 of food-borne pathogen bacillus cereus. The lyase involved in the invention has a nucleotide sequence shown as SEQ NO 1 and an amino acid sequence shown as SEQ NO 2. The phage lyase has high temperature tolerance and pH tolerance, can be used for prevention and control of bacillus cereus florabacteria, and the lyase can prevent and control pathogenic bacillus cereus under the condition of 4DEG C to 45DEG C. In addition, the lyase can also split pseudomonas aeruginosa. The invention provides a new enzyme preparation source for prevention and control of food-borne pathogen bacillus cereus, other bacillus cereus flora bacteria and pseudomonas aeruginosa at present.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to the preparation of a novel lysing enzyme capable of broad-spectrum lysing bacteriophage of Bacillus cereus, and its application in preventing and treating pathogenic Bacillus cereus. Background technique [0002] Bacillus cereus ( Bacillus cereus ) are widely distributed in a variety of environments, including dust, soil, and sewage, and also in the intestinal tracts of insects and animals; in addition, they are also common in plant foods and many raw and cooked foods (Drobniewski FA: Bacillus cereus and related species. Clin Microbiol Rev1993, 6(4):324-338). Bacillus cereus is a Gram-positive bacillus that produces spores. Due to their stress resistance, spores can survive in food sterilization processes such as high-temperature treatment, dehydration and drying, and are common food-borne opportunistic pathogens. Bacillus cereus can cause food poisoning in humans by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/80C12N15/55C12N1/21A61P31/04C12R1/19
CPCA61P31/04C12N9/80C12Y305/01028
Inventor 彭沁袁益辉
Owner HAINAN NORMAL UNIV
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