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Bacterium S2 for efficiently generating biosurfactant and fermentation culture medium thereof

A biological surface and active agent technology, applied in the biological field, can solve the problem of low production

Active Publication Date: 2011-11-23
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The currently reported microorganisms that produce biosurfactants are mainly Pseudomonas, and their production is relatively low

Method used

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  • Bacterium S2 for efficiently generating biosurfactant and fermentation culture medium thereof
  • Bacterium S2 for efficiently generating biosurfactant and fermentation culture medium thereof
  • Bacterium S2 for efficiently generating biosurfactant and fermentation culture medium thereof

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Embodiment Construction

[0020] Examples of the present invention are described below.

[0021] 1. Isolation and purification of bacterial strains

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Abstract

The invention relates to a bacterium S2 for efficiently generating biosurfactant and a fermentation culture medium thereof, belonging to the field of biotechnology. The bacterium S2 is gram-negative bacterium which is rod-shaped, is (6-8)*(8-12) micrometers in size and has no spores and capsules. The bacterial strain belongs to the pseudomonas aeruginosa through identifying by morphological and physio-biochemical characteristics and molecular biology, and the collection code is CGMCC No.3034. The best fermentation culture medium is a novel culture medium which takes 50g / L of rapeseed oil as the only carbon source. The rhamnolipid is the only fermentation product of the bacterial strain and has high yield. The CMC (Critical Micelle Concentration) value of the fermentation liquid is 0.25 g / L; compared with the common chemical surfactant, the bacterium has more obvious emulsification and durability on hydrophobic substances, such as oils and the like.

Description

1. Technical field [0001] The invention relates to a high-efficiency biosurfactant producing bacterium S2 and its fermentation medium, belonging to the field of biotechnology. 2. Background technology [0002] Biosurfactant is a kind of natural surfactant, which mainly refers to some metabolites produced by microorganisms under certain culture conditions with significantly reduced surface tension. Compared with chemically synthesized surfactants, they not only have the same characteristics of reducing surface tension, stabilizing emulsion and foaming, but also have environmental friendly characteristics that general chemically synthesized surfactants do not have, such as non-toxic, natural Biodegradable, ecological safety and other advantages. In addition, biosurfactants often have higher surface activity than chemically active agents, because the chemical structure of biosurfactants is much more complex and bulky than chemically synthesized surfactants, and a single molecu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/385
Inventor 周立祥任洁
Owner NANJING AGRICULTURAL UNIVERSITY
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