Pseudomonas aeruginosa and inoculant containing Pseudomonas aeruginosa, application thereof, and TNT degradation method

A technology of Pseudomonas aeruginosa and bacterial agents, applied in the field of bioremediation of organic matter pollution, to achieve high TNT degradation ability, high degradation rate, and good tolerance

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of Pseudomonas aeruginosa, the bacterium agent containing this bacterium and its application and a kind of method for degrading TNT in order to overcome the above-mentioned defect that exists in the bioremediation of TNT pollution

Method used

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  • Pseudomonas aeruginosa and inoculant containing Pseudomonas aeruginosa, application thereof, and TNT degradation method
  • Pseudomonas aeruginosa and inoculant containing Pseudomonas aeruginosa, application thereof, and TNT degradation method
  • Pseudomonas aeruginosa and inoculant containing Pseudomonas aeruginosa, application thereof, and TNT degradation method

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Embodiment

[0040] The present invention will be described in detail below through examples and comparative examples, but the present invention is not limited thereby.

[0041] The experimental methods in the following examples and comparative examples are conventional methods in the art unless otherwise specified. The experimental materials used in the following examples and comparative examples were purchased from conventional biochemical reagent stores unless otherwise specified.

Embodiment 1

[0045] This example is used to illustrate the liquid-phase TNT (with TNT as the single nitrogen source) degradation ability of Pseudomonas aeruginosa with the deposit number CGMCC NO: 10842 of the present invention.

[0046] Centrifuge the bacterial solution obtained in the preparation example to obtain the bacterial cells, then wash the bacterial cells with physiological saline and resuspend to obtain OD 600 3.0 normal saline bacterium liquid, the 1mL preservation number of the present invention obtained above is the physiological saline bacterium liquid (OD) of Pseudomonas aeruginosa NO:10842 600 =3.0) were inoculated into 100 mL of liquid medium M, and cultured at 37° C. in a shaker at 170 rpm, wherein the composition of liquid medium M was 5 g / L glucose+100 ppm TNT. Use high-performance liquid chromatography (the model of high-performance liquid chromatography is LC-20A, purchased from Shimadzu Corporation, Japan, the chromatographic column is Agilent ZORBAXSB-Aq (4.6mm × ...

Embodiment 2

[0048] This example is used to illustrate the liquid-phase TNT (containing ammonium salt in the liquid phase) degradation ability of Pseudomonas aeruginosa with the preservation number of CGMCC NO: 10842 of the present invention.

[0049] Centrifuge the bacterial solution obtained in the preparation example to obtain the bacterial cells, then wash the bacterial cells with physiological saline and resuspend to obtain OD 600 3.0 normal saline bacterium liquid, the 1mL preservation number of the present invention obtained above is the physiological saline bacterium liquid (OD) of Pseudomonas aeruginosa NO:10842 600 =3.0) were inoculated into 100mL liquid medium A, B, C, D respectively, and cultivated at 37°C in a 170rpm shaker, wherein the composition of liquid medium A was 5g / L glucose+100ppm TNT+1g / L (NH 4 ) 2 SO 4 . The composition of liquid medium B is 5g / L glucose+100ppm TNT+1g / L NH 4 Cl. The composition of liquid medium C is 5g / L glucose+100ppm TNT+1g / L NH 4 NO 3 . ...

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Abstract

The invention relates to the technical field of biologic restoration of organic matter pollution, and discloses a Pseudomonas aeruginosa and an inoculant containing the Pseudomonas aeruginosa, an application thereof, and a TNT degradation method. The Pseudomonas aeruginosa is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC NO:10842. The TNT degradation method is characterized in that the Pseudomonas aeruginosa with the preservation number of CGMCC NO:10842 and / or the inoculant containing the Pseudomonas aeruginosa with the preservation number of CGMCC NO:10842 are in contact with a TNT polluted sample to degrade TNT in the TNT polluted sample. The Pseudomonas aeruginosa has high TNT degradation ability, still has good tolerance and high degradability in high-salt environment, and plays a great role in the biological restoration process of TNT polluted soil or TNT-containing wastewater.

Description

technical field [0001] The invention relates to the technical field of bioremediation of organic pollution, in particular to a pseudomonas aeruginosa, a bacterial agent containing the bacteria and its application, and a method for degrading TNT. Background technique [0002] TNT (trinitrotoluene) is a common synthetic monomer explosive with mature technology, low price, stable performance and safe operation, so it is widely used in national defense and civilian fields. However, it has toxic, carcinogenic, and mutagenic effects on organisms, and TNT buried underground may also have major safety hazards. Therefore, if TNT compounds enter the environment during production, storage, use and destruction, they will seriously damage the local ecological environment and threaten the survival of human beings and other life, which has become a major focus of global attention. [0003] For TNT pollution, bioremediation technology has the characteristics of simple operation, high effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/385C02F101/38
Inventor 任立伟朗喆林森王培红
Owner 粮华生物科技(北京)有限公司
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