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Pseudomonas aeruginosa and application thereof in degrading zearalenone

A technology of Pseudomonas aeruginosa and zearalenone, applied in the biological field, can solve problems such as hepatotoxicity, tumorigenesis effect, genotoxicity and the like, and achieve the effect of good application prospect

Active Publication Date: 2014-08-13
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, ZEN also has immunotoxicity, liver toxicity, and genotoxicity, and has a certain impact on tumorigenesis

Method used

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  • Pseudomonas aeruginosa and application thereof in degrading zearalenone
  • Pseudomonas aeruginosa and application thereof in degrading zearalenone
  • Pseudomonas aeruginosa and application thereof in degrading zearalenone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the isolation and identification of bacteria

[0025] 1. Isolation of bacteria

[0026] (1) In June 2013, in the ultra-clean workbench, soil samples collected from the suburbs of Beijing were shaken in sterile distilled water for 15 minutes to prepare a bacterial suspension, and the shaker speed was 180rpm.

[0027] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, Strains with different colors and transparency were streaked and purified on plates, and the purified strains were spotted and applied to the degradation experiment of zearalenone. A strain with strong zearalenone degrading ability was obtained and named NS7.

[0028] 2. Identification

[0029] (1) According to the method described in "Berger's Bacteria Identification Handbook" (eighth edi...

Embodiment 2

[0037] Embodiment 2, the application of Pseudomonas aeruginosa NS7 in degrading zearalenone

[0038] 1. Culture activation of Pseudomonas aeruginosa NS7

[0039] Pseudomonas aeruginosa (Pseudomonas aeruginosa) NS7 (CGMCC No.8727) was inoculated in liquid NB medium, cultured by shaking at a temperature of 37°C and a rotation speed of 200 rpm (rotation radius: 20 mm) for 24 h, and the culture products were collected It is the NS7 culture medium, which is used for subsequent zearalenone degradation experiments.

[0040] 2. Degradation of zearalenone by Pseudomonas aeruginosa NS7

[0041] 1. Preparation of zearalenone:

[0042] The 5mg zearalenone standard substance (Sigma- , Cat. No. Z2125) was dissolved in 25 ml of chromatographically pure methanol to obtain a zearalenone solution with a concentration of 200 ppm.

[0043] 2. Degradation of zearalenone

[0044] Experimental group: 10 μl of zearalenone solution with a concentration of 200 ppm was placed in a 10 ml centrifuge t...

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Abstract

The invention discloses pseudomonas aeruginosa and an application thereof in degrading zearalenone. The preservation number of the pseudomonas aeruginosa is CGMCC No.8727. As a biologic material for degrading zearalenone, the pseudomonas aeruginosa is good in application prospect in no matter developing a novel biologic degrading bacterium agent or a biologic degrading bacterium-free preparation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Pseudomonas aeruginosa and its application in degrading zearalenone. Background technique [0002] Zearalenone (Zearalenone, ZEN) is an estrogenic mycotoxin produced by Fusarium fungus in the process of secondary metabolism. The types of grains contaminated by ZEN are very wide, such as wheat, barley, corn, oats, sorghum, rye, millet and related products of these grains. It is one of the most widely polluted Fusarium mycotoxins in the world. ZEN is toxic to both animals and humans, mainly affecting the reproductive function of animals, reducing the embryo survival rate of pregnant animals and the birth weight of newborn fetuses, leading to disordered animal reproductive function. People eating food contaminated by it can lead to diseases such as liver cancer, testicular cancer, esophageal cancer and precocious puberty. In addition, ZEN also has immunotoxicity, hepatotox...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015A23K1/00A62D3/02C12R1/385A62D101/28A23L5/20
Inventor 赵月菊刘阳娅娃·米妮·埃尔迪·富丽兰茨纳·桑格邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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