Method for screening biological catalyst strain for converting levo-fosfomycin into dextral-fosfomycin
A technology of levofosfomycin and dexfofomycin, which is applied to biochemical equipment and methods, microorganisms, microorganisms, etc., and can solve the problems of screening failures and large numbers of omissions
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[0027] First, inoculate the beef extract peptone liquid medium with Escherichia coli, cultivate it at 37°C for 24 hours, and prepare the bacterial suspension of Escherichia coli strain, so that the bacterial concentration is 10 8 -10 10 bacteria / ml, which is made into a high-concentration sensitive bacteria suspension.
[0028] Then the catalytic bacterial strain to be screened may be transformed into levofosfomycin (through these two characteristics of levofosfomycin resistance and / or dextrofosfomycin utilization, use conventional techniques to preliminarily judge whether the bacterial strain is Potentially transformable bacterial strains) were inoculated in the catalytic medium, and after 72 hours of shaking flask culture, the fermentation broth was drawn, and the bacteria in the fermentation broth were removed by filtration through a 0.45 μm pore size filter membrane to prepare a sterile fermentation broth of the bacterial strain to be screened.
[0029] Then, mix the abov...
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