Method for detecting candida albicans by applying multi-crossing constant-temperature amplification combining nano bio-sensing technology

Abstract

The invention provides a method for detecting candida albicans by applying a multi-crossing constant-temperature amplification combined with nano bio-sensing technology. The invention provides candidaalbicans ITSII amplification primer groups: CP1, CP2, F1, F2, C1, C2, D1, D2, R1 and R2; the method is used for marking hapten on a 5' end of an amplification primer C1 in multi-crossing constant-temperature amplification and marking biotin on a 5' end of an amplification primer D1; an amplification product aiming at a candida albicans specific sequence interval ITSII of the method can be visually detected through a gold nano bio-sensor; the method is convenient, rapid, sensitive and excellent and is suitable for being popularized to clinical detection of the candida albicans.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for detecting Candida albicans by applying multi-cross constant temperature amplification combined with nanobiological sensing technology. Background technique [0002] Candida albicans (Ca) is a member of the human intestinal flora and cannot reproduce in vitro. Candida albicans is also an opportunistic pathogenic yeast. More than 50% of human candida is caused by it. Candida albicans is one of the most common pathogens of invasive candidiasis, with a reported mortality rate of 40% in patients with systemic candidiasis. Hospital-acquired invasive candidiasis can kill 11,200 people a year in the United States, according to one estimate. However, the current gold standard method for the detection of C. albicans is based on phenotypic identification methods including culture, microscopic examination and biochemical identification, which usual...

AI Technical Summary

Problems solved by technology

This delayed diagnosis may lead to poor outcomes in patients with systemic fungal diseases

With the rapid development of nucleic acid diagnostic technology, some PCR-based diagnostic technologies (such as ordinary PCR technology, fluorescent PCR technology) are used for the rapid diagnosis of Candida albicans, but these methods rely on expensive and complicated equipment and high cost probe synthesis, and requires subsequent electrophoresis steps and skilled operators

Some grassroots laboratories cannot meet the above conditions, thus limiting the application of these technologies at the grassroots level

In addition, the current detection of Candida albicans using PCR method and real-time PCR method takes 2-4 hours, which is not conducive to rapid detection and emergency detection

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