Phosphorescent energy transfer system, synthetic method and use of system and detection method of thrombin

A technology of energy transfer and synthesis method, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, nanotechnology for materials and surface science, etc. It can solve problems such as not wide linear range, difficult protein determination, and large fluorescence background. Achieve the effect of high sensitivity, simple operation and avoid interference

Inactive Publication Date: 2014-06-25
ANHUI NORMAL UNIV
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Problems solved by technology

This type of method also measures the target protein through the change of the fluorescence signal, the fluorescence background is large, the linear range is not wide, and there are certain difficulties in the determination of low-concentration proteins

Method used

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  • Phosphorescent energy transfer system, synthetic method and use of system and detection method of thrombin
  • Phosphorescent energy transfer system, synthetic method and use of system and detection method of thrombin
  • Phosphorescent energy transfer system, synthetic method and use of system and detection method of thrombin

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Embodiment Construction

[0074] The present invention will be described in detail below according to the accompanying drawings, which is a preferred embodiment among various implementations of the present invention.

[0075] In this example, a nucleic acid aptamer with an amino-modified 5' end was immobilized on the surface of Mn-doped ZnS phosphorescent quantum dots wrapped in mercaptopropionic acid (MPA) through a dehydration condensation reaction, so that the labeled phosphorescent quantum dots were close to the carbon nanodots surface, the phosphorescence is quenched. Utilizing the strong binding effect between the nucleic acid aptamer and the thrombin, the phosphorescent group is far away from the surface of the carbon nano-dot, so that the phosphorescence intensity is restored, and is used to measure the amount of thrombin. In this experiment, for the first time, the principle of phosphorescence energy transfer between phosphorescent quantum dots and carbon nanodots was used to detect the conten...

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Abstract

The invention relates to a phosphorescent energy transfer system, a synthetic method and use of the system and a detection method of thrombin. The detection method comprises the following steps: fixing an aptamer with an amino-modified nucleic acid at 5'-end on the surface of an Mn doped ZnS phosphorescent quantum dot wrapped by mercaptopropionic acid (MPA) through dehydration and condensation reaction, wherein the marked phosphorescent quantum dot is close to the surface of a carbon nano point and phosphorescence is quenched; and by means of bond tight effect of the nucleic acid aptamer and the thrombin, separating the phosphorescent group from the surface of the carbon nano point, wherein the phosphorescent strength is recovered for measuring the amount of the thrombin. In the experiment, the content of the thrombin in a living body is detected by the phosphorescent energy transfer principle between the phosphorescent quantum dot and the carbon nano point, so that the detection selectivity and specificity are improved, the phosphorescent background is reduced, the quenching time is greatly shortened, the detection sensitivity is improved, the limit of detection is further low, and the phosphorescent signal collected is stable, thus the demand of microanalysis is met.

Description

technical field [0001] The invention relates to a phosphorescence energy transfer system, its synthesis method, application and thrombin detection method. Background technique [0002] The invasion of tumor tissue to surrounding tissues, the formation of metastasis, and the changes of tumor tissue and tumor patient's blood itself can all lead to changes in the patient's blood coagulation mechanism, resulting in different degrees of bleeding tendencies. Thrombin is an important physiological protease, which plays an important role in physiological and pathological processes such as blood coagulation, inflammation and wound healing, so the detection of thrombin is of great significance in medicine. The concentration and activity of thrombin (Thrombin) is an important index to measure the coagulation mechanism, and it is of great significance to reveal the mechanism of tumor occurrence, or as an early diagnosis, molecular typing, curative effect and prognosis judgment. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/57C09K11/65C09K11/02C01B31/02G01N21/64B82Y30/00B82Y40/00C01B32/05
Inventor 高峰张璐
Owner ANHUI NORMAL UNIV
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